Jamie Thompson

The development of ToF-SIMS for in-situ glycosaminoglycan analysis

Lorna K Milne, Jamie Thompson, Raina Ramnath, Simon Satchell et autres

Abstract Glycosaminoglycans (GAGs) are linear polysaccharides with essential roles in a myriad of biological processes. Despite their biological importance, methods to determine both spatial and compositional information is limited. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides spatially resolved compositional information of biological …

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Modelling co-development between the somites and neural tube in human trunk-like structures

Komal Makwana, Louise Tilley, Probir Chakravarty, Jamie Thompson et autres

Human stem cell-based embryo models provide experimentally amenable in vitro systems for developmental research. A key feature of embryo models is their multi-lineage differentiation, which allows for the study of tissue co-development. Here we develop human trunk-like structures that have morphologically organized …

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[PEER REVIEWED] Generation of human Trunk-like Structures (hTLS) from Human Pluripotent Stem Cells v1

Komal Makwana, Louise Tilley, Jamie Thompson, Ignacio Rodríguez-Polo et autres

Novel in vitro approaches are required to overcome the technical and ethical challenges associated with studying human embryogenesis. 3D stem cell-based embryo models are providing new insights on human biology from cultures of human pluripotent stem cells (hPSCs). This protocol describes the …

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Modelling co-development between the somites and neural tube in human Trunk-like Structures (hTLS)

Komal Makwana, Louise Tilley, Probir Chakravarty, Jamie Thompson et autres

Human stem cell-based embryo models have opened new avenues of research in development by providing experimentally amenable in vitro systems. One of the features of embryo models is their multilineage differentiation, which allows the co-development of, and interactions between, tissues. Here, we …

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Spliced-Vein Conduit Offers Better Patency and Limb Salvage Than Prosthetic Graft for Femoral-Tibial Bypasses in Critical Limb-Threatening Ischemia

Jamie Thompson, Renxi Li, Benjamin J. Pomy, John J. Ricotta et autres

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Transcriptional programs of Pitx2 and Tfap2a/Tfap2b controlling lineage specification of mandibular epithelium during tooth initiation

Fan Shao, An-Vi Phan, Wenjie Yu, Yuwei Guo et autres

How the dorsal-ventral axis of the vertebrate jaw, particularly the position of tooth initiation site, is established remains a critical and unresolved question. Tooth development starts with the formation of the dental lamina, a localized thickened strip within the maxillary and mandibular …

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Addressing Black Maternal Mortality

Lotus Alphonsus, Meythula Alphonsus, Jamie Thompson

Increasing rates of maternal morbidity and mortality is a growing concern in many industrialized countries. Data from US maternal mortality review committees indicate that more than 80% of these deaths are preventable (Trost et al., 2019). Various factors contributing to this issue …

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Below-knee Amputations Are Associated with Decreased Mortality and Reoperation in Elderly, Dependent Patients with Atherosclerotic Disease

Sheng Dong, Emanuela C. Peshel, Renxi Li, Jamie Thompson et autres

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Preinduction and Postdischarge Venothromboembolism Chemoprophylaxis Should Be Considered in Patients After Vascular Major Open Abdominal Procedures

Jamie Thompson, Renxi Li, Emanula C. Peshel, Anton N. Sidawy et autres

General Anesthesia Does Not Increase Mortality and Morbidity in High-risk Patients Undergoing Carotid Endarterectomy

Emanuela C. Peshel, Renxi Li, Yan Cheng, Jamie Thompson et autres

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Reconstituting human somitogenesis in vitro

Yoshihiro Yamanaka, Sofiane Hamidi, Kumiko Yoshioka-Kobayashi, Sirajam Munira et autres

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Performance evaluation of a non-invasive one-step multiplex RT-qPCR assay for detection of SARS-CoV-2 direct from saliva

Harry H. Jenkins, Ana A. Tellechea Lopez, Francesco Saverio Tarantini, Hannah Tomlin et autres

Polymerase chain reaction (PCR) has proven to be the gold-standard for SARS-CoV-2 detection in clinical settings. The most common approaches rely on nasopharyngeal specimens obtained from swabs, followed by RNA extraction, reverse transcription and quantitative PCR. Although swab-based PCR is sensitive, swabbing …

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