Cole A. DeForest

Boolean Logic-responsive FRET Biosensors via Genetically Encoded Autonomous Compilation

Hyerim Son, Jack W. Hoye, Michael Khawand, Murial L. Ross et autres

Förster resonance energy transfer (FRET) is commonly used to monitor protein-protein interactions in situ. The high spatiotemporal resolution and facile implementation inside complex molecular environments have spearheaded FRET's widespread adoption in biosensing. Despite these advantages, current FRET biosensors are largely restricted to …

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Modeling Tenascin-C-rich metastatic tumor niches in engineered hydrogel biomaterials

Aakanksha Jha, Elizabeth J. Lawlor, Cole A. DeForest

The extracellular matrix (ECM) plays a pivotal role in shaping tumor behavior by providing biochemical and biophysical cues to cancer cells. Traditional 2D culture systems fail to recapitulate this complexity, and in vivo systems do not readily allow for interrogation of how …

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Boolean Logic‐Based Control Over Recombinant Protein Biomaterial Degradability and Therapeutic Delivery

Murial L. Ross, Ryan Gharios, Shivani P. Kottantharayil, Cole A. DeForest

Recombinant protein-based biomaterials offer exciting opportunities in materials design and controlled therapeutic delivery. Though their precursors can be readily synthesized with near-perfect monodispersity and sequence specificity through scalable fermentation processes, recombinant protein materials have yet to achieve the same level of multi-stimuli-responsiveness …

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Intratumoral B7H3:CD3 Bispecific T-cell Engager Drives Localized T-cell Accumulation in Canine Sarcoma Patients

Yusuke Suita, Lisa S. Ang, Kenneth Brasel, Shelli M. Morris et autres

Abstract Background Bispecific T-cell Engagers (TCEs) targeting B7H3 (CD276) show promise for solid tumors but are limited by systemic toxicities and poor tumor penetration. Intratumoral (IT) delivery is proposed as a solution, but the safety and spatial pharmacodynamics (PD) remain poorly defined …

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Network Formation Dynamics in Thiol-ene Crosslinked Hyaluronic Acid Hydrogels: Design Principles for In Vitro Tissue Models

Kyley K Burkey, Yiwen Zheng, Kinsey Drake, Ryan Brady et autres

Abstract Hydrogels are widely used as three-dimensional cell culture systems to understand the impact of cellular mechanotransduction for tissue engineering applications. Photoinitiated thiol-ene click chemistry is a commonly utilized hydrogel crosslinking mechanism that provides spatial and temporal control over hydrogel network formation …

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A robust and user-agnostic step-emulsion platform for scalable microgel fabrication

Durante Pioche-Lee, Shiyu Yang, Xiaoqian Wang, Yoke Qi Ho et autres

Over the past decade, the integration of microgel-based granular hydrogels in biomedical technologies has experienced substantial growth due to the numerous benefits microgels offer. However, the inability to easily adopt uniform microgel fabrication workflows at scale constitutes a major bottleneck, or in …

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Abstract 758: ECM mimicking tunable 3D hydrogels to model Ewing sarcoma.

Aakanksha Jha, Cole A. DeForest, Elizabeth J. Lawlor

Abstract The extracellular matrix (ECM) is increasingly recognized as a critical regulator of tumor progression and metastasis. Despite its strong influence on tumor fate, the ECM remains poorly characterized within the solid tumor microenvironment. Ewing sarcoma (EwS), an aggressive bone and soft …

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Innovations and Opportunities in Graduate Education in Chemical Engineering

Christopher V.H.-H. Chen, Cole A. DeForest

De novo design of protein nanoparticles with integrated functional motifs

Cyrus M. Haas, Sanela Rankovic, Hanul K Lewis, Kenneth D. Carr et autres

Computational design of self-assembling proteins has long relied on pre-existing structures and sequences, fundamentally limiting control over their structural and functional properties. Recent machine learning-based methods have transformed our ability to design functional small de novo proteins and oligomers, yet methods to …

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Engineering complexity into protein-based biomaterials for biomedical applications

Nicole E. Gregorio, Cyrus M. Haas, Neil P. King, Cole A. DeForest

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Boolean Logic‐Based Controlled Release of Bioactive Proteins with Diversified Inputs

Murial L. Ross, Ryan Gharios, Shivani P. Kottantharayil, Annabella Li et autres

Abstract Stimuli‐responsive biomaterials hold great promise in controlled therapeutic delivery, tissue engineering, and biosensing applications. Recently, molecular assembly via autonomous compilation has been employed to create topologically specified protein cargos that can be site‐specifically tethered to and conditionally released from biomaterials following …

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Boolean Logic‐Based Controlled Release of Bioactive Proteins with Diversified Inputs

Murial L. Ross, Ryan Gharios, Shivani P. Kottantharayil, Annabella Li et autres

Stimuli-responsive biomaterials hold great promise in controlled therapeutic delivery, tissue engineering, and biosensing applications. Recently, molecular assembly via autonomous compilation has been employed to create topologically specified protein cargos that can be site-specifically tethered to and conditionally released from biomaterials following user-programmable …

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