Daosheng Huang

Functional screening of TCR-like antibodies using STAR-T cell library for cancer immunotherapy

Yi Li, Daosheng Huang, Chang Liu, Zhixiao Zhou et autres

Abstract Adoptive T-cell therapies engineered with T-cell receptors (TCRs) or TCR-like antibodies have shown considerable promise in cancer immunotherapy. However, identifying tumor antigen-specific TCR-like antibodies, particularly against human leukocyte antigen-presented neoantigens, remains challenging. Here, we present a function-based, rather than affinity-based, antibody …

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Dynamics of Cell Fate Decisions during Chemically Induced Multi‐Lineage Trans‐Differentiation at Single‐Cell Level

E Weigao, Lijiang Fei, Jingjing Wang, Xinru Wang et autres

Cell trans-differentiation offers a powerful means to manipulate cell identities. By exposing cells to a combination of small molecules (SMs), cell trans-differentiation can be induced in a simple and cost-effective manner. However, a comprehensive atlas detailing chemical-induced cell trans-differentiation across multiple cell …

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Converting TCR-based chimeric antigen receptor STAR into dual-specific targeting receptor for cancer immunotherapy

Yu Li, Zhixiao Zhou, Hanyang Yu, Yue Liu et autres

Chimeric antigen receptor (CAR) T cell therapy has achieved great success in treating hematopoietic malignancies; however, post-therapy relapse remains a challenge. Traditionally, multi-specific CAR engineering requires precise arrangement of single-chain variable fragments (scFvs), which can lead to aggregation issues when assembled linearly. …

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Identification of virus epitopes and reactive T-cell receptors from memory T cells without peptide synthesis

Lihui Wang, Runda Xu, Daosheng Huang, Pai Peng et autres

Identifying epitopes and their corresponding T-cell receptor (TCR) sequences is crucial in the face of rapidly mutating viruses. Peptide synthesis is often required to confirm the exact epitope sequences, which is time-consuming and expensive. In this study, we introduce a scalable workflow …

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TCR-mimicking STAR conveys superior sensitivity over CAR in targeting tumors with low-density neoantigens

Daosheng Huang, Yi Li, Keyong Sun, Zhixiao Zhou et autres

Targeting tumor-specific neoantigens holds promise for cancer immunotherapy, but their ultra-low expression on tumor cells poses challenges for T cell therapies. Here, we found that chimeric antigen receptors (CARs) exhibit 10–100 times lower sensitivity than T cell receptors (TCRs) when targeting human …

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Glioma-derived ANXA1 suppresses the immune response to TLR3 ligands by promoting an anti-inflammatory tumor microenvironment

Yu Zheng, Haihui Jiang, Naixue Yang, Shaoping Shen et autres

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Case report: A rare multidrug-resistant Escherichia coli causes fatal neonatal meningoencephalitis

Qingyun Zhou, Zhifei Zhan, Furong Huang, Menghua Zhao et autres

Neonatal meningitis is rare but devastating disease. Multidrug-resistant (MDR, multi-drug resistant) bacteria are a major global health risk. We report an Escherichia coli meningitis isolate with multiple resistance patterns and unusual serotype (O75) that caused sudden neonatal death. The isolate was resistant …

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Efficacy of pp65‐specific TCR‐T cell therapy in treating cytomegalovirus infection after hematopoietic stem cell transplantation

Guangna Liu, Hua Chen, Xingyu Cao, Lemei Jia et autres

Abstract Cytomegalovirus (CMV) infection remains a major cause of mortality after hematopoietic stem cell transplantation (HSCT). Current treatments, including antiviral drugs and adoptive cell therapy with CMV‐specific cytotoxic T lymphocytes (CTLs), only show limited benefits in patients. T‐cell receptor (TCR)‐T cell therapy …

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Chimeric STAR receptors using TCR machinery mediate robust responses against solid tumors

Yue Liu, Guangna Liu, Jiasheng Wang, Zheyu Zheng et autres

Chimeric antigen receptor T (CAR-T) cell therapies have demonstrated high response rate and durable disease control for the treatment of B cell malignancies. However, in the case of solid tumors, CAR-T cells have shown limited efficacy, which is partially attributed to intrinsic …

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CXCR2‐modified CAR‐T cells have enhanced trafficking ability that improves treatment of hepatocellular carcinoma

Guangna Liu, Hongli Zheng, Daosheng Huang, Fei Yu et autres

Unlike hematological malignancies, solid tumors have proved to be less susceptible to chimeric antigen receptor (CAR)-T cell therapy, which is partially caused by reduced accumulation of therapeutic T cells in tumor site. Since efficient trafficking is the precondition and pivotal step for …

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Author response for "CXCR2‐modified CAR‐T cells have enhanced trafficking ability that improves treatment of hepatocellular carcinoma"

Guangna Liu, Hongli Zheng, Daosheng Huang, Fei Yu et autres

Mapping the Mouse Cell Atlas by Microwell-Seq

Xiaoping Han, Renying Wang, Yincong Zhou, Lijiang Fei et autres