Xiuwen Huo

Transcriptomic Analysis Based on RNA-Seq Technology Reveals the Molecular Mechanisms of Sunflower (Helianthus annuus L.) Response to Salt Stress

Y ZHANG, Jiaxin Xie, Shuchun Guo, Mengjie Liu et autres

Background/Objectives: Sunflower (Helianthus annuus L.) is one of the four major oil crops worldwide and possesses strong stress tolerance. However, salt stress remains limiting in the improvement of sunflower yield and quality. Methods: In this study, the salt-tolerant cultivar P50 and salt-sensitive …

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Genome-Wide Identification and Expression Profiling of Plasma Membrane-Localized SWEET Gene Family Associated with Sugar Transport During Yam Tuber Development

Na Li, Yanfang Zhang, Xiuwen Huo, Linan Xing et autres

This study provides the first comprehensive genome-wide identification and characterization of the SWEET gene family in yam (Dioscorea rotundata), integrating structural bioinformatics, gene expression profiling, and functional validation to explore its roles in sucrose transport and tuber development. A total of 19 …

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Genome-Wide Identification, Plasma Membrane Localization, and Functional Validation of the SUT Gene Family in Yam (Dioscorea cayennensis subsp. rotundata)

Na Li, Yanfang Zhang, Xiuwen Huo, Linan Xing et autres

Yam (Dioscorea cayennensis subsp. rotundata,hereafter referred to as Dioscorea rotundata) is a staple tropical tuber crop with notable nutritional and economic value. Its development and yield depend on efficient sucrose allocation from source tissues. Sucrose transporters (SUTs), a conserved family of membrane …

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Reference genes selection and validation in yam by real-time quantitative polymerase chain reaction

Linan Xing, Yanfang Zhang, Yanping Xing, Mingran Ge et autres

Gene expression pattern analysis plays a crucial role in omics research, as it helps us understand the regulatory mechanisms of gene expression and the associated biological processes. Real-time quantitative polymerase chain reaction (q-PCR) is an efficient method for studying gene expression. Reliable …

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DoDELLA-GAI2 Integrates Gibberellin and Ethylene Signaling to Regulate Chinese Yam (Dioscorea opposita) Tuber Development

Mingran Ge, Yanfang Zhang, Yanping Xing, Linan Xing et autres

Yam (Dioscorea opposita) tuber development is a complex process regulated by various phytohormones, with gibberellin (GA) playing a crucial role. However, the underlying mechanisms and interaction of GA with other phytohormone pathways on yam tuber development remain incompletely understood. This study investigated …

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Mechanism of fatty acid synthesis metabolism during tuber swelling period of Chinese yam

Na Li, Yanfang Zhang, Xiuwen Huo, Shuchun Guo et autres

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Identification of WRKY gene family in Dioscorea opposita Thunb. reveals that DoWRKY71 enhanced the tolerance to cold and ABA stress

Linan Xing, Yanfang Zhang, Mingran Ge, Lingmin Zhao et autres

WRKY transcription factors constitute one of the largest plant-specific gene families, regulating various aspects of plant growth, development, physiological processes, and responses to abiotic stresses. This study aimed to comprehensively analyze the WRKY gene family of yam (Dioscorea opposita Thunb.), to understand …

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Identification of Dioscorea opposite Thunb. CDPK gene family reveals that DoCDPK20 is related to heat resistance

Yuanli Gao, Yanfang Zhang, Xiang Ji, Jinxin Wang et autres

Temperature affects the growth and yield of yam (Dioscorea opposite Thunb.), and calcium-dependent protein kinases (CDPKs) play an important role in the plant stress response. However, there has been a lack of system analyses of yam’s CDPK gene family. In this study, …

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Overexpression of DoBAM1 from Yam (Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco

Lingmin Zhao, Yanfang Zhang, Ying Shao, Linan Xing et autres

β-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 DoBAM members were identified in yam (Dioscorea opposita Thunb.). A novel β-amylase gene BAM1, (named DoBAM1), was isolated from yam varieties Bikeqi and Dahechangyu. …

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Identification of the WRKY gene family and the functional analysis of DoWRKY71 in yam (Dioscorea opposita Thunb.)

Linan Xing, Yanfang Zhang, Mingran Ge, Lingmin Zhao et autres

Abstract Background:Dioscorea opposita Thunb. is one of the top 10 most important edible tuber and root plants worldwide, have been widely cultivated for nutritional and medicinal purposes. Yam is susceptible to various biological and abiotic stresses that will affect the yield and …

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Transcriptome Analysis Reveals the Accumulation Mechanism of Starch in Chinese Yam (Dioscorea opposite Thunb.) Tubers Using RNA-Seq and Iso-Seq

Yuanli Gao, Yanfang Zhang, Jinxin Wang, Xiaohua Sun et autres

The starch composition of tubers affects the quality and yield of yam, and it is of great significance to deeply investigate the mechanism of starch accumulation in yam. In this study, two yam varieties with significant differences in starch content, Bikeqi (Dioscorea …

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Transciptome Analysis Molecular Mechanism of Starch Synthesis During Tuber Development in Chinese Yam (Dioscorea opposita)

Yanfang Zhang, Shuchun Guo, Ying Shao, Lingmin Zhao et autres

Yam (Dioscorea opposita) is a kind of vegetables with important nutritional, medicinal and economic value. To reveal the relationship between starch synthesis and gene expression in yam tubers at gene transcription level, transcriptome profiling was conducted by RNA-Seq in Bikeqi yam (Dioscorea …

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