Surya Pratap Singh

In Cellulo Observation of De Novo -Synthesized Nucleotide by Spectral Tracing of Glutamine-Derived Nitrogen Metabolism (QN-STIM) in Cancer Cells

Jiro Karlo, Surya Pratap Singh

Abstract De novo nucleotide biosynthesis is directly linked to glutamine-derived nitrogen metabolism in mammalian cells. It is a fundamental process tightly tied to cellular growth, proliferation, and metabolic reprogramming, particularly in cancer cells. Direct detection and imaging of glutamine-derived nitrogen metabolism at …

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Decoding the Biochemical Complexities Using Raman Spectroscopy

Jiro Karlo, Dhananjaya Giriraju, Nilkamal Mahanta, Surya Pratap Singh

Obtaining biochemical information and monitoring the biomolecules in complex biological systems and environments remains a challenge and requires methods that can detect biomolecules. Raman spectroscopy has emerged as a powerful analytical tool for biochemical analysis owing to its ability to provide label-free, …

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Real-Time, Label-Free Monitoring of Microbial Pectin Degradation and Carbon Assimilation Using Raman Spectroscopy

Sujal Sahu, Jiro Karlo, Chaya P. Patil, Basavaraj Hungund et autres

Pectin is one of the major polysaccharides produced in plants, and a valuable source of renewable carbon. In this study, we have established a Raman spectroscopy-based framework to study the degradation of pectin by Bacillus subtilis BGN4 and directly trace the metabolic …

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Bridging Trauma and Parkinson’s Disease: Mechanisms, Models, and Biomarkers of Post-Traumatic Parkinsonism

Singh Ankit Satyaprakash, Nitesh Kumar Gupta, Shekhar Singh, Rajaneesh Kumar Gupta et autres

One of the most prevalent neurodegenerative diseases, Parkinson's disease (PD), is generally discussed in terms of aging, genetic predisposition, and environmental exposures. Nonetheless, there is growing evidence that both isolated severe traumatic events and repetitive mild traumatic brain injury may play a …

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In cellulo observation of newly synthesized nucleotide by spectral tracing of glutamine-derived nitrogen metabolism (QN-STIM) in cancer cells

Jiro Karlo, Surya Pratap Singh

De-novo nucleotide biosynthesis is directly linked to glutamine-derived nitrogen metabolism in mammalian cells. It is a fundamental process tightly tied to cellular growth, proliferation, and metabolic reprogramming, particularly in cancer cells. Direct detection and imaging of glutaminederived nitrogen metabolism at the single-cell …

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Ensemble-Enhanced Raman Spectroscopy: A Feature-Driven Framework for Microbial Classification and Spectral Interpretation

Ritam Dadhara, Dimple Saikia, Waghmare Ashwin Mahendra, Jiro Karlo et autres

Rapid and accurate identification of bacterial pathogens is essential in clinical diagnostics, environmental monitoring, and food safety control. Here, we report an ensemble-enhanced Raman spectroscopy approach for the classification and spectral interpretation of nine bacterial species belonging to both Gram-positive and Gram-negative …

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Raman Hyperspectral Imaging for Resolving Sub-Particle Chemical Heterogeneity in Environmentally Derived Microplastics

Jiro Karlo, Priyanshu, Aman Chourasia, Sudip Mitra et autres

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Chemometric Raman Spectroscopy for Sugar Profiling in Fresh and Commercially Packaged Tender Coconut Water

Darshan Chikkanayakanahalli Mukunda, Ritam Dadhara, Jiro Karlo, Surya Pratap Singh

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Visualization of de novo synthesized nascent purine and pyrimidine using carbon isotope imaging and spectral tracing (CIIST) in single microbial cell

Jiro Karlo, Tejas Ajit Mhaiskar, Hrishikesh Ravindra Karande, Surya Pratap Singh

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Raman stable isotope probing (SIP): A novel bio-analytical tool

Jiro Karlo, Rishikesh Pandey, Surya Pratap Singh

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Peaks to Pixels: Multivariate Unmixing of Hyperspectral Resonance Raman Image for Mapping Newly Synthesized Carotenoids in Micro-factories

Jiro Karlo, Nilesh Kumar Dewangan, Surya Pratap Singh

Carotenoids are naturally occurring biomolecules with potent antioxidant activity that humans must obtain through their diet, as they cannot synthesize them endogenously. These pigments are naturally produced by plants and microbes, including red yeast cells that act as micro-factories, particularly genera such …

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Combating Antimicrobial Resistance: Spectroscopy Meets Machine Learning

Dimple Saikia, Ritam Dadhara, Cebajel Tanan, Prajwal Avati et autres

One of the major health challenges that humans have been facing for the last few decades is antimicrobial resistance (AMR), where antibiotics stop responding to infections and, thereby, take a considerable amount of time to cure them while increasing mortality rates. There …

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