Carlos F. Ng

LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs

Carlos F. Ng, Deepak Krishnamurthy, Andres Dextre, Aymeric Chorlay et autres

CRISPR diagnostics enable sensitive detection of infectious diseases, with the RNA endonuclease Cas13a providing specific, amplification-free RNA detection through collateral trans-cleavage of fluorescent reporters. However, background cleavage from unbound enzyme, contaminating nucleases, and unsynchronized initiation of reactions limits assay sensitivity and interpretability. …

us (code pays fourni par la source)

Programmable kinetic barcoding for multiplexed RNA detection with Cas13a

Sungmin Son, Amy Lyden, Carlos F. Ng, Andres Dextre et autres

Rapid identification of viral infections and specific variants in patient samples requires a simple and multiplexed RNA detection method that does not rely on DNA sequencing. Although recent direct detection assays based on CRISPR-Cas13a offer rapid RNA detection by avoiding reverse transcription …

kr, us (code pays fourni par la source)

NTDscope: A multi-contrast portable microscope for disease diagnosis

María Díaz de León Derby, Zaina L. Moussa, Carlos F. Ng, Abdul Bhuiya et autres

Accurate diagnostics are essential for disease control and elimination efforts. However, access to diagnostics for neglected tropical diseases (NTDs) is hindered by limited healthcare infrastructure in many NTD-endemic regions, as well as by reliance on time- and labor-intensive diagnostic methods, such as …

us, de, Gabon, ca, Côte d’Ivoire, bd (code pays fourni par la source)

NTDscope: A multi-contrast portable microscope for disease diagnosis

María Díaz de León Derby, Zaina L. Moussa, Carlos F. Ng, Abdul Bhuiya et autres

Abstract Accurate diagnostics are essential for disease control and elimination efforts. However, access to diagnostics for neglected tropical diseases (NTDs) is hindered by limited healthcare infrastructure in many NTD-endemic regions, as well as by reliance on time- and labor-intensive diagnostic methods, such …

us, de, Gabon, ca, Côte d’Ivoire, bd (code pays fourni par la source)

Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants

Helena de Puig, Rose Lee, Devora Najjar, Xiao Tan et autres

The COVID-19 pandemic highlights the need for diagnostics that can be rapidly adapted and deployed in a variety of settings. Several SARS-CoV-2 variants have shown worrisome effects on vaccine and treatment efficacy, but no current point-of-care (POC) testing modality allows their specific …

us (code pays fourni par la source)

Increased Phosphorylation of ACTN4 Leads to Podocyte Dysfunction and Focal Segmental Glomerulosclerosis Mimicking Disease-Causing Mutations

Di Feng, Mukesh Kumar, Jan Muntel, Susan B. Gurley et autres

Background: Genetic mutations in ACTN4 have been linked to focal segmental glomerulosclerosis (FSGS) in humans through cytoskeletal disruption and impairment in podocyte response to mechanical stress. ACTN4 is phosphorylated at S159 in podocytes, but the effect of this post-translational modification on podocyte …

us, ch (code pays fourni par la source)

Phosphorylation of ACTN4 Leads to Podocyte Vulnerability and Proteinuric Glomerulosclerosis

Di Feng, Mukesh Kumar, Jan Muntel, Susan B. Gurley et autres

Significance Statement Although genetic mutations in α -actinin-4 (ACTN4) are linked with proteinuric glomerulosclerosis in humans, the effect of post-translational modifications is unknown. The authors show that ACTN4—an actin crosslinking cytoskeletal protein—is phosphorylated at serine 159 (S159) in podocytes. Compared with wild-type …

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Author Correction: On-chip recapitulation of clinical bone marrow toxicities and patient-specific pathophysiology

David B. Chou, Viktoras Frismantas, Yuka Milton, Rhiannon David et autres

us, gb, pt (code pays fourni par la source)

Robotic fluidic coupling and interrogation of multiple vascularized organ chips

Richard Novák, Susan Marquez, Debarun Das, Aaron Delahanty et autres

us, se, il, pt, de (code pays fourni par la source)

On-chip recapitulation of clinical bone marrow toxicities and patient-specific pathophysiology

David B. Chou, Viktoras Frismantas, Yuka Milton, Rhiannon David et autres

us, gb, pt (code pays fourni par la source)

A robotic platform for fluidically-linked human body-on-chips experimentation

Richard Novák, Susan L. Clauson, Debarun Das, Aaron Delahanty et autres

Here we describe of an ‘Interrogator’ instrument that uses liquid-handling robotics, a custom software package, and an integrated mobile microscope to enable automated culture, perfusion, medium addition, fluidic linking, sample collection, and in situ microscopic imaging of up to 10 Organ Chips …

us, pt, de (code pays fourni par la source)

Human bone marrow disorders recapitulated in vitro using organ chip technology

David B. Chou, Viktoras Frismantas, Yuka Milton, Rhiannon David et autres

Abstract Understanding human bone marrow (BM) pathophysiology in the context of myelotoxic stress induced by drugs, radiation, or genetic mutations is of critical importance in clinical medicine. However, study of these dynamic cellular responses is hampered by the inaccessibility of living BM …

us, gb, pt (code pays fourni par la source)