Yu. M. Konstantinov

Short Interrupted Repeats Cassette ensembles of plant nuclear genomes reflect evolutionary route of species

Igor Vladimirovich Gorbenko, Dmitriy Scherbakov, Karolina Mikhailovna Zverintseva, Yu. M. Konstantinov

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Using the Rejuvenation Phenomenon to Obtain Vegetative Progeny of Trees

В. Н. Шмаков, V. I. Belkov, Yu. M. Konstantinov

Abstract Vegetative propagation provides the opportunity to scale up valuable plant material in the shortest possible time. It acquires particular importance in the cultivation of tree crops while preserving valuable biological and morphological varietal characteristics of individual specimens. The use of this …

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Arabidopsis GDH1 and GDH2 genes double knock-out results in a stay-green phenotype during dark-induced senescence

Elena Yu. Garnik, Daria V. Vilyanen, Anfisa A. Vlasova, Vladislav I. Tarasenko et autres

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Phylogeny and characterization of endophytic bacteria isolated from the rhizosphere of pea seedlings (Pisum sativum L.)

Л. Е. Макарова, Yu. A. Markova, Yu. V. Zaytseva, A. A. Bychkova et autres

We have previously shown the ability of endophytic bacteria to move out of the pea plant seedling roots (Pisum sativum L.) into the rhizosphere. In this study, six distinct bacterial strains were isolated from the root growth medium during the cultivation of …

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Phylogeny and Characterization of Endophytic Bacteria Isolated from the Rhizosphere of Pea Seedlings (Pisum Sativum L.)

Л. Е. Макарова, Yu. A. Markova, Yu. V. Zaytseva, A. A. Bychkova et autres

Abstract Previously it was shown that endophytic bacteria had the ability to move out of the roots of pea plant seedlings (Pisum sativum L.) into the rhizosphere. In this study, six distinct bacterial strains were isolated from the root growth medium during …

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Overexpression of RPOTmp Being Targeted to Either Mitochondria or Chloroplasts in Arabidopsis Leads to Overall Transcriptome Changes and Faster Growth

Igor Vladimirovich Gorbenko, Vladislav I. Tarasenko, Elena Yu. Garnik, Tatiana V. Yakovleva et autres

The transcription of Arabidopsis organellar genes is performed by three nuclear-encoded RNA polymerases: RPOTm, RPOTmp, and RPOTp. The RPOTmp protein possesses ambiguous transit peptides, allowing participation in gene expression control in both mitochondria and chloroplasts, although its function in plastids is still …

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Inactivation of the TIM complex components leads to a decrease in the level of DNA import into Arabidopsis mitochondria

Tatiana A. Tarasenko, K. D. Elizova, Vladislav I. Tarasenko, Milana V. Koulintchenko et autres

The phenomenon of DNA import into mitochondria has been shown for all major groups of eukaryotes. In plants and animals, DNA import seems to occur in different ways. It has been known that nucleic acids enter plant organelles through alternative channels, depending …

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Short Interrupted Repeat Cassette (SIRC)—Novel Type of Repetitive DNA Element Found in Arabidopsis thaliana

Igor Vladimirovich Gorbenko, Ivan Sergeevich Petrushin, A. B. Shcherban, Yuriy Lvovich Orlov et autres

nuclear genome. SIRCs are represented by short direct repeats interrupted by diverse DNA sequences. The maxima of SIRC's distribution are located within pericentromeric regions. We suggest that originally SIRC was a special case of the complex internal structure of the miniature inverted …

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Differential Expression of a Foreign Gene in Arabidopsis Mitochondria In Organello

Vladislav I. Tarasenko, Tatiana A. Tarasenko, Igor Vladimirovich Gorbenko, Yu. M. Konstantinov et autres

Genetic transformation of higher eukaryote mitochondria in vivo is an unresolved and important problem. For efficient expression of foreign genetic material in mitochondria, it is necessary to select regulatory elements that provide a high level of transcription and transcript stability. This work …

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Differential Expression of a Foreign Gene in Arabidopsis Mitochondria in organello

Vladislav I. Tarasenko, Tatiana A. Tarasenko, Igor Vladimirovich Gorbenko, Yu. M. Konstantinov et autres

Genetic transformation of higher eukaryote mitochondria in vivo is an unresolved and important problem. For efficient expression of foreign genetic material in mitochondria, it is necessary to select regulatory elements that provide a high level of transcription and transcript stability. This work …

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Comparative Analysis of Sterol Composition of Embryogenic and Nonembryogenic Cell Lines of Larix sibirica Ledeb.

Natalya V. Semenova, В. Н. Шмаков, Yu. M. Konstantinov, Л. В. Дударева

Abstract The cell lines of Siberian larch (Larix sibirica Ledeb.), possessing different embryogenic potential, were studied. Qualitative and quantitative content of sterol components was comparatively analyzed in their tissues with the help of gas chromatography-mass spectrometry. Significant differences in these parameters were …

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DECREASE IN THE CONTENT OF REACTIVE OXYGEN SPECIES DURING INACTIVATION OF THE RESPIRATORY COMPLEX I IN ARABIDOPSIS CELLS

Vladislav I. Tarasenko, Elena Yu. Garnik, В. Н. Шмаков, Yu. M. Konstantinov

Background. The mitochondrial electron transport chain, in particular the respiratory complex I, is one of the main sources of reactive oxygen species (ROS) in living cells. Suppression of the complex I activity in human cells through chemical inhibition or mutations that disrupt …

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