Nikhil Chauhan

A next-generation human lymphatic filariasis vaccine candidate, rBmHAXT, for clinical development

Sean A. Gray, Jennifer Knight Davis, Conrad M Puff-Carter, Vishal Khatri et autres

Abstract This study was conducted to yield a robust and scalable manufacturing process for a candidate vaccine for human lymphatic filariasis (LF) - a tropical parasitic infection transmitted by mosquitoes. In previous studies, we demonstrated that removing an affinity purification tag from …

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A next-generation human lymphatic filariasis vaccine candidate, rBmHAXT, for clinical development

Nithila Saravanan, Sean A. Gray, Jennifer Davis, Conrad M. Puff-Carter et autres

Abstract This study was conducted to develop a robust, scalable manufacturing process for the candidate vaccine rBmHAXT for human lymphatic filariasis (LF). During scale-up production, rBmHAXT showed significant antigen aggregation leading to a loss of purified vaccine antigens. This project aims to …

The Minimally Invasive 1-Hour Esophageal String Test Monitors Therapeutic Changes in Mucosal Inflammation in Eosinophilic Esophagitis

Steven J. Ackerman, Amir F. Kagalwalla, Zhaoxing Pan, Joshua B. Wechsler et autres

INTRODUCTION: Endoscopy, standard-of-care for monitoring eosinophilic esophagitis (EoE), assesses mucosal inflammation. The Esophageal String Test (EST), a minimally invasive swallowed capsule and immunoassays, quantifies EoE inflammation. We determined whether the EST/EoEScore can monitor disease in patients undergoing treatment. METHODS: Thirty-three samples from …

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Immunomodulation of streptozotocin induced Type 1 diabetes mellitus in mouse model by Macrophage migration inhibitory factor-2 (MIF-2) homologue of human lymphatic filarial parasite, Wuchereria bancrofti

Ishwar S Singh, S. L. Hoti, Nikhil Chauhan, Rajesh Kumar Joshi et autres

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Pre-clinical development of a vaccine for human lymphatic filariasis

Valeria Melendez, Cheri Turner, Vishal Khatri, Jenn Davis et autres

This study was conducted to optimize a fusion protein vaccine for translational development as a vaccine against the human tropical parasitic infection, lymphatic filariasis (LF). The vaccine candidate, His-tagged r Bm HAXT was developed previously in our laboratory and was tested in …

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Parasite Cystatin: Immunomodulatory Molecule with Therapeutic Activity against Immune Mediated Disorders

Vishal Khatri, Nikhil Chauhan, Ramaswamy Kalyanasundaram

The use of parasites or their products for treating chronic inflammation associated diseases (CIADs) has generated significant attention recently. Findings from basic and clinical research have provided valuable information on strengthening the notion that parasites' molecules can be developed as biotherapeutic agents. …

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Fecundity of adult female worms were affected when Brugia malayi infected Mongolian gerbils were immunized with a multivalent vaccine (rBmHAXT) against human lymphatic filarial parasite

Vishal Khatri, Nikhil Chauhan, Ramaswamy Kalyanasundaram

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Peptide fragments of cystatin protein from filarial parasite has potent anti-inflammatory effect on DSS-induced colitis in mouse

Vishal Khatri, Nikhil Chauhan, S B Prasanna Kumar, Ramaswamy Kalyanasundaram

Abstract The therapeutic potential of filarial parasite derived recombinant cystatin was reported previously by our group in the mouse model of colitis. We showed that cystatin-treatment elevated IL-10+FoxP3+Tregs, IgM+B1a cells and AAMs in the colon and peritoneal cavity. In this study, we …

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Macrophage migration inhibitory factor-2 of Wuchereria bancrofti suppressed the DSS-induced colitis in a mouse model by promoting alternative activation of peritoneal macrophages and increasing the number of Treg cells in the colon

Nikhil Chauhan, Vishal Khatri, S B Prasanna Kumar, Valeria Malendez et autres

Abstract In this study, we cloned the homologue of macrophage migration inhibitory factor (MIF) secreted by the human filarial parasite Wuchereria bancrofti (rWbaMIF-2) and evaluated its potential in ameliorating inflammation in a dextran sulfate sodium salt (DSS)-induced colitis model. DSS administration resulted …

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Wuchereria bancrofti macrophage migration inhibitory factor‐2 (rWbaMIF‐2) ameliorates experimental colitis

Shriram Ramani, Nikhil Chauhan, Vishal Khatri, Connie Vitali et autres

Immunomodulatory molecules produced by helminth parasites are receiving much attention recently as novel therapeutic agents for inflammation and autoimmune diseases. In this study, we show that macrophage migration inhibitory factor (MIF) homologue from the filarial parasite, Wuchereria bancrofti (rWbaMIF-2), can suppress inflammation …

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Advances in Vaccine Development for Human Lymphatic Filariasis

Ramaswamy Kalyanasundaram, Vishal Khatri, Nikhil Chauhan

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Cystatin from Filarial Parasites Suppress the Clinical Symptoms and Pathology of Experimentally Induced Colitis in Mice by Inducing T-Regulatory Cells, B1-Cells, and Alternatively Activated Macrophages

Nalini Bisht, Vishal Khatri, Nikhil Chauhan, Ramaswamy Kalyanasundaram

Potential alternative therapeutic strategies for immune-mediated disorders are being increasingly recognized and are studied extensively. We previously reported the therapeutic potential of Brugia malayi derived recombinant cystatin (rBmaCys) in attenuating clinical symptoms of experimental colitis. The aim of this study was to …

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