Alessia Giannini

HIV-1 Sub-subtype A6 Remains Susceptible to Second-Generation Integrase Inhibitors With Limited Emergence of Resistance in Vitro

Francesco Saladini, Alessia Giannini, Federica Giammarino, Anastasiia Antonova et autres

Background: HIV-1 sub-subtype A6 is predominant in Eastern Europe and was associated with increased risk of treatment failure with the long-acting cabotegravir plus rilpivirine regimen. In this study, we aimed to evaluate the in vitro susceptibility and the genetic barrier to resistance …

it, ru, de, se (code pays fourni par la source)

Evaluation of a Neck Cream Developed to Enhance Nitric Oxide Availability in Aging Skin.

Deanne Mraz Robinson, Joely Kaufman, Alessia Giannini, Syed Minhaj Rahman et autres

BACKGROUND: To meet the unique needs of aging skin of the neck, a new neck cream that enhances nitric oxide availability has been developed to visibly improve signs of aging and overall quality of skin. METHODS: The primary objective of this dual …

Multicenter evaluation of a topical hyaluronic acid serum

Deanne Mraz Robinson, J. Sanchez Vega, Melanie D. Palm, Michaela Bell et autres

BACKGROUND: A new hyaluronic acid (HA) formulation was developed based on high molecular weight (MW) compounds used on the surface of the skin while using peptides to stimulate the high MW HA production by fibroblasts and keratinocytes from within the skin layers. …

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External quality assessment of HIV-1 DNA quantification assays used in the clinical setting in Italy

Ilaria Vicenti, Filippo Dragoni, Alessia Giannini, Anna Casabianca et autres

Abstract Total cell-associated HIV-1 DNA is a surrogate marker of the HIV-1 reservoir, however, certified systems for its quantification are not available. The Italian HIV DNA Network was launched to validate HIV-1 DNA quantification methods in use at University and Hospital labs. …

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In vitro susceptibility of HIV-1 CRF02_AG to temsavir, the active compound of the attachment inhibitor fostemsavir

Francesco Saladini, Alessia Giannini, Federica Giammarino, Adele Boccuto et autres

Fostemsavir, a prodrug of the active compound temsavir, has been recently approved as the first-in-class HIV-1 gp120-directed attachment inhibitor for salvage therapy in subjects with MDR HIV-1. Since the drug target is highly polymorphic, a key concern is whether different HIV-1 lineages …

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Maraviroc as a potential HIV-1 latency-reversing agent in cell line models and ex vivo CD4 T cells

Ilaria Vicenti, Filippo Dragoni, Martina Monti, Claudia Maria Trombetta et autres

Recent studies have suggested that the CCR5 antagonist maraviroc (MVC) may exert an HIV-1 latency reversal effect. This study aimed at defining MVC-mediated induction of HIV-1 in three cell line latency models and inex vivoCD4 T cells from six patients with suppressed …

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In vitro cross-resistance to doravirine in a panel of HIV-1 clones harbouring multiple NNRTI resistance mutations

Francesco Saladini, Federica Giammarino, Behnaz A Hosseini, Alessia Giannini et autres

OBJECTIVES: Doravirine is a recently licensed HIV-1 NNRTI with improved efficacy, pharmacokinetics and safety profile compared with efavirenz and limited cross-resistance with rilpivirine and etravirine. In this in vitro study, cross-resistance to doravirine was analysed in a representative panel of NNRTI-resistant clones. …

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DDX3X inhibitors, an effective way to overcome HIV-1 resistance targeting host proteins

Annalaura Brai, Valentina Riva, Francesco Saladini, Claudio Zamperini et autres

The huge resources that had gone into Human Immunodeficiency virus (HIV) research led to the development of potent antivirals able to suppress viral load in the majority of treated patients, thus dramatically increasing the life expectancy of people living with HIV. However, …

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In vitro susceptibility to fostemsavir is not affected by long-term exposure to antiviral therapy in MDR HIV-1-infected patients

Francesco Saladini, Alessia Giannini, Federica Giammarino, Franco Maggiolo et autres

OBJECTIVES: Fostemsavir is the prodrug of the HIV-1 attachment inhibitor temsavir and is currently under clinical assessment in heavily treatment-experienced patients with limited therapeutic options. We evaluated the genotypic and phenotypic susceptibility to temsavir in a panel of samples collected from patients …

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5,6-Dihydroxypyrimidine Scaffold to Target HIV-1 Nucleocapsid Protein

Savina Malancona, Mattia Mori, Paola Fezzardi, Marisabella Santoriello et autres

The HIV-1 nucleocapsid (NC) protein is a small basic DNA and RNA binding protein that is absolutely necessary for viral replication and thus represents a target of great interest to develop new anti-HIV agents. Moreover, the highly conserved sequence offers the opportunity …

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Development of a Cell-Based Immunodetection Assay for Simultaneous Screening of Antiviral Compounds Inhibiting Zika and Dengue Virus Replication

Ilaria Vicenti, Filippo Dragoni, Alessia Giannini, Federica Giammarino et autres

Practical cell-based assays can accelerate anti-Zika (ZIKV) and anti-dengue (DENV) virus drug discovery. We developed an immunodetection assay (IA), using a pan-flaviviral monoclonal antibody recognizing a conserved envelope domain. The final protocol includes a direct virus yield reduction assay (YRA) carried out …

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(Thia)calixarenephosphonic Acids as Potent Inhibitors of the Nucleic Acid Chaperone Activity of the HIV-1 Nucleocapsid Protein with a New Binding Mode and Multitarget Antiviral Activity

Nicolas Humbert, Lesia Kovalenko, Francesco Saladini, Alessia Giannini et autres

The nucleocapsid protein (NC) is a highly conserved protein that plays key roles in HIV-1 replication through its nucleic acid chaperone properties mediated by its two zinc fingers and basic residues. NC is a promising target for antiviral therapy, particularly to control …

fr, ua, it, lu (code pays fourni par la source)