High contrast fluorescence polarization microscopy through double tagged photoswitchable fluorescent proteins
Lukas Jonathan Münker, Manuel Hohgardt, Andreas Albrecht, Dominik Pfennig et autres
We demonstrate that rigid anchoring of fluorescent proteins through double tagging (FPs) in living cells can significantly enhance the contrast in fluorescence polarization microscopy (FPM) by locking the transition dipole moment orientations to the sample's structures. We applied double tagging of reversibly …
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3 citations
npj Imaging