Rocco D’Antuono

Directional X‐Ray Scattering and Microtomography for Laboratory‐Based Imaging of Fibrous Materials and Biological Tissues

Carlos Navarrete-León, Alvaro Jose Gonzalez-Grajales, Harry Allan, Adam Doherty et autres

X‐ray microtomography enables nondestructive three‐dimensional imaging, yet conventional attenuation contrast provides limited sensitivity to microstructural organization below the system's spatial resolution. Dark‐field imaging addresses this limitation by detecting scattering from unresolved structures, enabling indirect access to sub‐resolution features across large fields of …

gb (code pays fourni par la source)

GloBIAS survey results – An insight into the global bioimage analysis community

Laura Rodríguez de la Ballina, Christa G Walther, Nicholas D. Condon, Alessandro Felder et autres

There is a global need for bioimage analysis (BIA) as advances in life sciences increasingly rely on cutting-edge imaging systems that have dramatically expanded the complexity and dimensionality of biological images. Turning these data into scientific discoveries requires scientists with effective data …

no, at, de, au, gb, cz, us, jp, dk, se, pt, ar, fr (code pays fourni par la source)

Mechanistic studies of autophagic cargo recruitment and membrane shaping through in vitro reconstitution

Wenxin Zhang, Thomas Litschel, Anne Schreiber, Rocco D’Antuono et autres

Macroautophagy/autophagy, a conserved intracellular catabolic pathway, removes deleterious cytosolic material to maintain homeostasis and survival. Upon autophagy induction, a unique double-membraned structure, the phagophore, forms and engulfs cytosolic material, the cargo, as it closes to become an autophagosome. Mammalian Atg8-family proteins (ATG8s) …

Mechanistic studies of autophagic cargo recruitment and membrane shaping through in vitro reconstitution

Wenxin Zhang, Thomas Litschel, Anne Schreiber, Rocco D’Antuono et autres

Macroautophagy/autophagy, a conserved intracellular catabolic pathway, removes deleterious cytosolic material to maintain homeostasis and survival. Upon autophagy induction, a unique double-membraned structure, the phagophore, forms and engulfs cytosolic material, the cargo, as it closes to become an autophagosome. Mammalian Atg8-family proteins (ATG8s) …

Mechanistic studies of autophagic cargo recruitment and membrane shaping through in vitro reconstitution

Wenxin Zhang, Thomas Litschel, Anne Schreiber, Rocco D’Antuono et autres

Macroautophagy/autophagy, a conserved intracellular catabolic pathway, removes deleterious cytosolic material to maintain homeostasis and survival. Upon autophagy induction, a unique double-membraned structure, the phagophore, forms and engulfs cytosolic material, the cargo, as it closes to become an autophagosome. Mammalian Atg8-family proteins (ATG8s) …

gb, us (code pays fourni par la source)

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila

Mélodie Ambroset, Cesar Augusto Valades‐Cruz, Libert Brice Tonfack, Rocco D’Antuono et autres

Reproducibility challenges in bioimage analysis are common. The proliferation of tools and workflows creates a complex ecosystem that is difficult to reproduce without deliberate, explicit reporting and support. We assess the reproducibility of Gehrels et al.(2023) as a representative bioimage analysis workflow. …

fr, cn, Cameroun, gb, ch (code pays fourni par la source)

GloBIAS: strengthening the foundations of bioimage analysis

Agustín A. Corbat, Christa G Walther, Laura Rodríguez de la Ballina, Nicholas D. Condon et autres

se, ar, at, de, no, au, gb, cz, us, jp, dk, fr, pt (code pays fourni par la source)

GloBIAS mission and working groups figure

Agustin Andres Corbat, Christa G Walther, Laura Rodríguez de la Ballina, Nicholas D. Condon et autres

GloBIAS figure (available in .ai, .pdf and .png formats).

GloBIAS mission and working groups figure

Agustin Andres Corbat, Christa G Walther, Laura Rodríguez de la Ballina, Nicholas D. Condon et autres

GloBIAS figure (available in .ai, .pdf and .png formats).

Laboratory-based x-ray microtomography with directional dark-field sensitivity

Carlos Navarrete-León, Alvaro Jose Gonzalez-Grajales, Harry Allan, Adam Doherty et autres

We demonstrate dark-field x-ray microtomography in a compact, laboratory-based system capable of resolving attenuation, phase, and anisotropic scattering signals with micrometer-scale resolution across centimetre-scale samples. The method is based on two-directional beam tracking (2DBT), which requires only a single optical element and …

A structural and regulatory framework for Atg9-containing vesicle formation and their Atg1-dependent remodelling during autophagy initiation

Carmen Oeo‐Santos, Ella Knüpling, Colin T. Davis, Xin Meng et autres

Abstract Autophagy is a complex intracellular degradation pathway that depends on the coordinated interplay between the core autophagy machinery and diverse membrane sources to drive the de novo formation of double-membrane vesicles, known as autophagosomes. Golgi-derived Atg9-containing vesicles are essential for this …

gb (code pays fourni par la source)

AI-based hardware and software tools in microscopy to boost research in immunology and virology

Diego Morone, Rocco D’Antuono

The integration of computational advances in microscopy has enhanced our ability to visualise immunological events at scales. However, data generated with these techniques is often complex, multi-dimensional, and multi-modal. Data science and artificial intelligence (AI) play a key role in untangling the …

ch, gb (code pays fourni par la source)