Frank Sargent

Genetic dissection of formate hydrogenlyase-2 function: a metalloenzyme involved in bacterial hydrogen production

Thomas C. P. Reed, Alexander J. Finney, Magali Roger, Frank Sargent

Abstract Formate hydrogenlyases are metalloenzymes that can either produce molecular hydrogen gas or use H 2 to convert carbon dioxide to formic acid, thus potentially contributing doubly to a sustainable energy future. The structure of formate hydrogenlyase reveals a membrane-bound redox enzyme …

gb, us, fr (code pays fourni par la source)

Implications for methenamine hippurate use in recurrent urinary tract infection management: Formaldehyde resistance and altered urinary composition

Niamh C. Hodgkinson, Tabarak Al-Rubaye, Thomas C. P. Reed, Catherine Mowbray et autres

Methenamine is a urinary antiseptic used to prevent urinary tract infections (UTI) via conversion to formaldehyde in the urinary tract. Methenamine hippurate (MH) is non-inferior compared to antibiotic (ABX) prophylaxis to manage recurrent UTI (rUTI) as demonstrated in the clinical trial ALTAR. …

gb (code pays fourni par la source)

A Comprehensive Genetic Toolkit for Rapid and High-Resolution Engineering of Metabolic Pathways in Zymomonas mobilis

Melissa Poma, Matteo Marchetti, Cathal Burns, Andreas Zannetou et autres

Abstract The limited availability of reliable genetic tools has constrained the precise engineering of Zymomonas mobilis , a promising bacterium for the sustainable bioproduction of fuels and commodity chemicals. Here, we present a comprehensive, modular genetic toolkit that enables high-resolution, combinatorial control …

gb, it (code pays fourni par la source)

Development of a Universal Platform for the Heterologous Expression of Bidirectional [Ni–Fe]-Hydrogenases in E. coli

Dominik L. Siebert, Frank Sargent, Ammar Al‐Shameri, Volker Sieber

High Resolution Image Download MS PowerPoint Slide Bidirectional [Ni–Fe]-hydrogenases are useful tools for integrating hydrogen into existing chemical processes by utilizing H 2 to regenerate expensive cofactors such as NAD(P)H. One enzyme broadly applied to this purpose is the soluble [Ni–Fe]-hydrogenase from …

de, gb, au (code pays fourni par la source)

A lysis less ordinary: The bacterial Type 10 Secretion System

M. A. R. Chowdhury, Phillip James Stansfeld, Frank Sargent

gb (code pays fourni par la source)

Corrigendum: Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria

Yunhan Wu, Andrew Bell, Gavin H. Thomas, David N. Bolam et autres

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher and we support and invest in the microbiology community, to the benefit of everyone. This supports our principal goal to develop, expand and strengthen the networks …

gb (code pays fourni par la source)

Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria

Yunhan Wu, Andrew Bell, Gavin H. Thomas, David N. Bolam et autres

A corrigendum of this article has been published full details can be found at 10.1099/mic.0.001476 Sialic acid (Sia) transporters are critical to the capacity of host-associated bacteria to utilise Sia for growth and/or cell surface modification. While N-acetyl-neuraminic acid (Neu5Ac)-specific transporters have …

gb (code pays fourni par la source)

Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria

Yunhan Wu, Andrew Bell, Gavin H. Thomas, David N. Bolam et autres

ABSTRACT Sialic acid (Sia) transporters are critical to the capacity of host-associated bacteria to utilise sialic acid for growth and/or cell-surface modification. While N-acetyl-neuraminic acid (Neu5Ac)-specific transporters have been studied extensively, little is known on transporters dedicated to anhydro-sialic acid forms such …

gb, us (code pays fourni par la source)

Formate hydrogenlyase, formic acid translocation and hydrogen production: dynamic membrane biology during fermentation

Katharina Peters, Frank Sargent

Formate hydrogenlyase-1 (FHL-1) is a complex-I-like enzyme that is commonly found in gram-negative bacteria. The enzyme comprises a peripheral arm and a membrane arm but is not involved in quinone reduction. Instead, FHL-1 couples formate oxidation to the reduction of protons to …

gb (code pays fourni par la source)

Physiology of formate hydrogenlyase: a complex-I-like enzyme from Proteobacteria

Frank Sargent

gb (code pays fourni par la source)

A paean to the ineffable Marjory Stephenson

Frank Sargent, R. Gary Sawers

It is now 75 years since Marjory Stephenson became the second President of the Society for General Microbiology (SGM). Around the time of her death at the end of 1948 many articles appeared extolling Marjory Stephenson's contribution to the fields of Biochemistry …

gb, de (code pays fourni par la source)

Hydrogen production in the presence of oxygen by Escherichia coli K-12

George D. Metcalfe, Frank Sargent, Michael Hippler

Escherichia coli is a facultative anaerobe that can grow in a variety of environmental conditions. In the complete absence of O 2 , E. coli can perform a mixed-acid fermentation that contains within it an elaborate metabolism of formic acid. In this …

gb (code pays fourni par la source)