Arjun Thapaliya

MdfA is a novel ClpC adaptor protein that functions in the developing Bacillus subtilis spore

Shawn C. Massoni, Nicola J. Evans, Ingo Hantke, Colleen Fenton et autres

Bacterial protein degradation machinery consists of chaperone–protease complexes that play vital roles in bacterial growth and development and have sparked interest as novel antimicrobial targets. ClpC–ClpP (ClpCP) is one such chaperone–protease complex, recruited by adaptors to specific functions in the model bacterium …

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iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells

Habib Bouguenina, Stephanos Nicolaou, Yann‐Vaï Le Bihan, Elizabeth A. Bowling et autres

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MdfA is a novel ClpC adaptor protein that functions in the developing Bacillus subtilis spore

Shawn C. Massoni, Nicola J. Evans, Ingo Hantke, Colleen Fenton et autres

ABSTRACT Bacterial protein degradation machinery consists of chaperone-protease complexes that play vital roles in bacterial growth and development and have sparked interest as novel antimicrobial targets. ClpC-ClpP (ClpCP) is one such chaperone-protease, recruited by adaptors to specific functions in the model bacterium …

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Determination of Ligand-Binding Affinity (Kd) Using Transverse Relaxation Rate (R2) in the Ligand-Observed 1H NMR Experiment and Applications to Fragment-Based Drug Discovery

Manjuan Liu, Amin Mirza, Craig McAndrew, Arjun Thapaliya et autres

High Resolution Image Download MS PowerPoint Slide High hit rates from initial ligand-observed NMR screening can make it challenging to prioritize which hits to follow up, especially in cases where there are no available crystal structures of these hits bound to the …

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iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells

Habib Bouguenina, Stephanos Nicolaou, Yann‐Vaï Le Bihan, Elizabeth A. Bowling et autres

To address the limitation associated with degron based systems, we have developed iTAG, a synthetic tag based on IMiDs/CELMoDs mechanism of action that improves and addresses the limitations of both PROTAC and previous IMiDs/CeLMoDs based tags. Using structural and sequence analysis, we …

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Investigating the phosphinic acid tripeptide mimetic DG013A as a tool compound inhibitor of the M1-aminopeptidase ERAP1

Birgit Wilding, Elisa Pasqua, N. Chessum, Olivier A. Pierrat et autres

ERAP1 is a zinc-dependent M1-aminopeptidase that trims lipophilic amino acids from the N-terminus of peptides. Owing to its importance in the processing of antigens and regulation of the adaptive immune response, dysregulation of the highly polymorphic ERAP1 has been implicated in autoimmune …

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Structural complexity of the co-chaperone SGTA: a conserved C-terminal region is implicated in dimerization and substrate quality control

Santiago Martínez‐Lumbreras, E. Krysztofinska, Arjun Thapaliya, Alessandro Spilotros et autres

BACKGROUND: Protein quality control mechanisms are essential for cell health and involve delivery of proteins to specific cellular compartments for recycling or degradation. In particular, stray hydrophobic proteins are captured in the aqueous cytosol by a co-chaperone, the small glutamine-rich, tetratricopeptide repeat-containing …

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Backbone assignment of SGTA TPR_C-terminal(deltaQ) domains

Santiago Martinez Lumbreras, Arjun Thapaliya, Rivka L. Isaacson

Structure and Interactions of the TPR Domain of Sgt2 with Yeast Chaperones and Ybr137wp

E. Krysztofinska, Nicola J. Evans, Arjun Thapaliya, James W. Murray et autres

Small glutamine-rich tetratricopeptide repeat-containing protein 2 (Sgt2) is a multi-module co-chaperone involved in several protein quality control pathways. The TPR domain of Sgt2 and several other proteins, including SGTA, Hop, and CHIP, is a highly conserved motif known to form transient complexes …

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A novel RNA polymerase‐binding protein that interacts with a sigma‐factor docking site

Anna F. Wang-Erickson, Padraig Deighan, Shanshan Chen, Kelsey Barrasso et autres

Summary Sporulation in Bacillus subtilis is governed by a cascade of alternative RNA polymerase sigma factors. We previously identified a small protein Fin that is produced under the control of the sporulation sigma factor σF to create a negative feedback loop that …

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Biophysical studies of SGTA, a protein involved in quality control

Arjun Thapaliya

SGTA interacts with the proteasomal ubiquitin receptor Rpn13 via a carboxylate clamp mechanism

Arjun Thapaliya, Yvonne Nyathi, Santiago Martínez‐Lumbreras, E. Krysztofinska et autres

The fate of secretory and membrane proteins that mislocalize to the cytosol is decided by a collaboration between cochaperone SGTA (small, glutamine-rich, tetratricopeptide repeat protein alpha) and the BAG6 complex, whose operation relies on multiple transient and subtly discriminated interactions with diverse …

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