Pantong Yao

A hypoxia-sensitive medullary nucleus modulates cerebral blood flow via a disynaptic pathway to cortex

Karishma Chhabria, J.C. Duckworth, Pantong Yao, Arash Fassihi Zakeri et autres

Abstract The RVLM (rostral ventral lateral medulla) region of the brainstem is implicated as a controller of both systemic and cerebral blood flow (CBF). Past studies leave open the question of how the RVLM stabilizes CBF in awake animals. Here, we focus …

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Glutamate indicators with increased sensitivity and tailored deactivation rates

Abhi Aggarwal, Adrian Negrean, Yang Chen, Rishyashring R. Iyer et autres

Understanding how neurons integrate signals from thousands of input synapses requires methods to monitor neurotransmission across many sites simultaneously. The fluorescent protein glutamate indicator iGluSnFR enables visualization of synaptic signaling, but the sensitivity, scale and speed of such measurements are limited by …

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Spatiotemporal development of expanding bacterial colonies driven by emergent mechanical constraints and nutrient gradients

Harish Kannan, Hui Sun, M.R. Warren, Tolga Çağlar et autres

Bacterial colonies growing on solid surfaces can exhibit robust expansion kinetics, with constant radial growth and saturating vertical expansion, suggesting a common developmental program. Here, we study this process for Escherichia coli cells using a combination of modeling and experiments. We show …

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Glutamate indicators with increased sensitivity and tailored deactivation rates

Kaspar Podgorski, Abhi Aggarwal, Adrian Negrean, Yang Chen et autres

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Glutamate indicators with increased sensitivity and tailored deactivation rates

Abhi Aggarwal, Adrian Negrean, Yang Chen, Rishyashring R. Iyer et autres

Abstract Identifying the input-output operations of neurons requires measurements of synaptic transmission simultaneously at many of a neuron’s thousands of inputs in the intact brain. To facilitate this goal, we engineered and screened 3365 variants of the fluorescent protein glutamate indicator iGluSnFR3 …

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Construction and use of an adaptive optics two-photon microscope with direct wavefront sensing

Pantong Yao, Rui Liu, Thomas Broggini, Martin Thunemann et autres

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Spatiotemporal development of growth and death zones in expanding bacterial colonies driven by emergent nutrient dynamics

Harish Kannan, Paul Sun, Tolga Çağlar, Pantong Yao et autres

ABSTRACT Bacterial colony growth on hard agar is commonplace in microbiology; yet, what occurs inside a growing colony is complex even in the simplest cases. Robust colony expansion kinetics featuring a linear radial growth and a saturating vertical growth indicates a common …

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Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

Abhi Aggarwal, Rui Liu, Yang Chen, Amelia J Ralowicz et autres

The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit low in vivo signal-to-noise ratios, saturating activation kinetics and exclusion from postsynaptic densities. Using a multiassay screen in bacteria, soluble protein and cultured …

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Guide to the construction and use of an adaptive optics two-photon microscope with direct wavefront sensing

Pantong Yao, Rui Liu, Thomas Broginni, Martin Thunemann et autres

Two-photon microscopy, combined with appropriate optical labeling, has enabled the study of structure and function throughout nervous systems. This methodology enables, for example, the measurement and tracking of sub-micrometer structures within brain cells, the spatio-temporal mapping of spikes in individual neurons, and …

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Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

Abhi Aggarwal, Rui Liu, Yang Chen, Amelia J Ralowicz et autres

Abstract The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit saturating activation kinetics and are excluded from post-synaptic densities, limiting their ability to distinguish synaptic from extrasynaptic glutamate. Using a multi-assay screen …

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Dentate granule cells encode auditory decisions after reinforcement learning in rats

Jia Shen, Pantong Yao, Shaoyu Ge, Qiaojie Xiong

Auditory-cued goal-oriented behaviors requires the participation of cortical and subcortical brain areas, but how neural circuits associate sensory-based decisions with goal locations through learning remains poorly understood. The hippocampus is critical for spatial coding, suggesting its possible involvement in transforming sensory inputs …

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Cortical ensemble activity discriminates auditory attentional states

Pantong Yao, Jia Shen, Liang Chen, Shaoyu Ge et autres

Selective attention modulates sensory cortical activity. It remains unclear how auditory cortical activity represents stimuli that differ behaviorally. We designed a cross-modality task in which mice made decisions to obtain rewards based on attended visual or auditory stimuli. We recorded auditory cortical …

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