George T. Shubeita

Directing traffic: MAPs, motors, and cellular logistics

Ibtissem Nabti, George T. Shubeita

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Seek-a-Seq : Optical detection of target nucleic acids with attomolar sensitivity

Anand Kant Das, Alam Saj, Hin Hark Gan, Kristin C. Gunsalus et autres

Abstract We introduce Seek-a-Seq, an enzyme-free, PCR amplification-free, optical method to detect microbial nucleic acids based on fluorescent readout of hybridization between target sequences and complementary probes immobilized on the surface of customized sample chambers. We demonstrate the ability to detect ultralow …

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BPS2025 - Enzyme-free, amplification-free, ultrasensitive optical detection of target nucleic acids

Anand Kant Das, Alam Saj, Hin Hark Gan, Kristin C. Gunsalus et autres

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On the use of thermal forces to probe kinesin’s response to force

Chuan Hsiung Chang, Tiantian Zheng, Guilherme Nettesheim, Hayoung Song et autres

The stepping dynamics of cytoskeletal motor proteins determines the dynamics of cargo transport. In its native cellular environment, a molecular motor is subject to forces from several sources including thermal forces and forces ensuing from the interaction with other motors bound to …

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The microtubule associated protein 4 (MAP4) sweeps organelles towards the nucleus by weakening the kinesin motor

Ibtissem Nabti, Babu Reddy Janakaloti Narayanareddy, Rachid Rezgui, Wenqi Wang et autres

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The ubiquitous microtubule-associated protein 4 (MAP4) controls organelle distribution by regulating the activity of the kinesin motor

Ibtissem Nabti, Babu J.N. Reddy, Rachid Rezgui, Wenqi Wang et autres

Regulation of organelle transport by molecular motors along the cytoskeletal microtubules is central to maintaining cellular functions. Here, we show that the ubiquitous tau-related microtubule-associated protein 4 (MAP4) can bias the bidirectional transport of organelles toward the microtubule minus-ends. This is concurrent …

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Detecting DNA-Ligand Electrostatic Interactions With a FRET-Based Probe

C.U. Murade, George T. Shubeita

DNA-ligand interactions are dominated by electrostatics as DNA is a highly charged molecule at physiological conditions. Here, we present a FRET-based sensor which can optically report on these interactions between DNA and charged ligands.

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Entropy and Entropic Forces to Model Biological Fluids

R.M. Gutiérrez, George T. Shubeita, C.U. Murade, Jianfeng Guo

Living cells are complex systems characterized by fluids crowded by hundreds of different elements, including, in particular, a high density of polymers. They are an excellent and challenging laboratory to study exotic emerging physical phenomena, where entropic forces emerge from the organization …

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Entropy and Entropic Forces to Model Biological Fluids

Rafael Gutiérrez, George T. Shubeita, C.U. Murade, Jianfeng Guo

Living cells are complex systems that may be characterized by fluids crowded by hundreds of different elements in particular by a high density of polymers; they are an excellent and challenging laboratory to study exotic emerging physical phenomena where entropic forces emerge …

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FRET-Based Probe for High-Throughput DNA Intercalator Drug Discovery and In Vivo Imaging

C.U. Murade, Samata Chaudhuri, Ibtissem Nabti, Hala Zahreddine Fahs et autres

Molecules that bind DNA by intercalating its bases remain among the most potent cancer therapies and antimicrobials due to their interference with DNA-processing proteins. To accelerate the discovery of novel intercalating drugs, we designed a fluorescence resonance energy transfer (FRET)-based probe that …

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Entropy and entropic forces to model biological fluids

Rafael Gutiérrez, C.U. Murade, Jianfeng Guo, George T. Shubeita

Biological systems tend to exhibit common organizational patterns despite they diversity and different spatial and temporal scales. Living cells are complex systems that may be characterized by fluids crowded by hundreds of different elements in particular by a high density of polymers; …

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Macromolecular crowding acts as a physical regulator of intracellular transport

Guilherme Nettesheim, Ibtissem Nabti, C.U. Murade, Gabriel R. Jaffe et autres

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