Makoto Hidaka

Improvement of the nitrogenase activity in Escherichia coli that expresses the nitrogen fixation-related genes from Azotobacter vinelandii

Yusuke Ito, Daisuke Yoshidome, Makoto Hidaka, Yasuko Araki et autres

The transfer of nitrogen fixation (nif) genes from diazotrophs to non-diazotrophic hosts is of increasing interest for engineering biological nitrogen fixation. A recombinant Escherichia coli strain expressing Azotobacter vinelandii 18 nif genes (nifHDKBUSVQENXYWZMF, nifiscA, and nafU) were previously constructed and showed nitrogenase …

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Glutamate production from aerial nitrogen using the nitrogen-fixing bacterium Klebsiella oxytoca

Daisuke Yoshidome, Makoto Hidaka, Toka Miyanaga, Yusuke Ito et autres

Abstract Glutamate is an essential biological compound produced for various therapeutic and nutritional applications. The current glutamate production process requires a large amount of ammonium, which is generated through the energy-consuming and CO2-emitting Haber–Bosch process; therefore, the development of bio-economical glutamate production …

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Characterization of the Azotobacter vinelandii nitrogenase complex expressed in Escherichia coli toward further activity improvement

Yusuke Ito, Daisuke Yoshidome, Makoto Hidaka, Yasuko Araki et autres

We previously constructed an Escherichia coli strain expressing 16 nitrogen fixation (nif) and 2 nif-related genes from Azotobacter vinelandii and improved nitrogenase activity to some extent by enhancing NifH-related functions. In the present study, we analyzed the formation of dinitrogenase, a heterotetrameric …

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Glutamate production from aerial nitrogen using nitrogen-fixing bacterium Klebsiella oxytoca

Makoto Nishiyama, Daisuke Yoshidome, Makoto Hidaka, Toka Miyanaga et autres

Abstract Glutamate is an essential biological compound produced for various practical uses. However, the current glutamate production process requires a large amount of ammonium produced through the most energy-consuming and CO2-emitting Haber–Bosch process; hence, the development of bioeconomical glutamate-producing processes has long …

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The Virgo High-Resolution CO Survey: III. NGC 4254

Yoshiaki Sofue, Jin Koda, Hiroyuki Nakanishi, Makoto Hidaka

Abstract We present high-angular-resolution (${1\rlap {.}{}^{\mathrm {\prime \prime }}5}$-${5{}^{\mathrm {\prime \prime }}}$) interferometer observations of the ${{12\atop} \mathrm{CO}}$ ($J=1 \hbox{--} 0$) line emission in the central region of the SA(s)c galaxy NGC 4254 (M 99). The observations were obtained using the Nobeyama …

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Ram-Pressure Effects on Dense Molecular Arms in the Central Regions of Spiral Galaxies by Intracluster Medium

Makoto Hidaka, Yoshiaki Sofue

We investigated ram-pressure effects by an intracluster wind on an inner disk of spiral galaxies by a hydrodynamical simulation. Even if the wind is mild and not strong enough to strip the gas disk, the ram pressure disturbs orbits of the inter-arm …

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Inherited chilling tolerance in somatic hybrids of transgenic Hibiscus rosa-sinensis x transgenic Lavatera thuringiaca selected by double-antibiotic resistance

Alejandro Vazquez-Thello, Jun Yang, Makoto Hidaka, Takeshi Uozumi

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Inherited chilling tolerance in somatic hybrids of transgenic Hibiscus rosa-sinensis� transgenic Lavatera thuringiaca selected by double-antibiotic resistance

Li Yang, Makoto Hidaka, Takeshi Uozumi, Alejandro Vazquez‐Tello

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NifA Protein SynthesizedIn VitroBinds to the Upstream Activator Sequence in the Promoter of theKlebsiella oxytoca nifB Gene

Youngmi Kim, Makoto Hidaka, Haruhiko Masaki, Teruhiko Beppu et autres

Young-Mi Kim, Makoto Hidaka, Haruhiko Masaki, Teruhiko Beppu, Takeshi Uozumi; NifA Protein Synthesized In Vitro Binds to the Upstream Activator Sequence in

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Major anthocyanin of the flowers of hibiscus (Hibiscus rosa-sinensis L.).

Yasuyuki Nakamura, Makoto Hidaka, Haruhiko Masaki, Haruo Seto et autres

The red pigments contained in red flowers of the Hibiscus species are anthocyanins, and have been widely used as coloring agents.Particularly, the red anthocyanins present in the calyces of roselle {Hibiscus sabdariffa L.) have been used as food colorants, and their major …

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