J.B. Minari

In Silico Evaluation of Quercetin-Chitosan Conjugate Binding to Mutant EGFR in Non-Small Cell Lung Cancer: A Molecular Docking and Dynamics Study

Godwin Offumobi Ogar, J.B. Minari, Luqman A. Adams, Babamusa Nafisat Ogbown

Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related deaths worldwide. Although quercetin exhibits promising anticancer properties through predicted epidermal growth factor receptor (EGFR) modulation, its clinical utility is restricted by poor bioavailability and rapid metabolism. This study aimed to …

Nigéria (code pays fourni par la source)

Comparative Sub-Acute Toxicological Profiling of Cisplatin and Benzo(a)pyrene in Sprague-Dawley Rats

Godwin Offumobi Ogar, J.B. Minari, Lukman A. Adams

CRISPR-Cas9 Mediated Knockout of LacZ Gene in Escherichia Coli for Enhanced Production of Asparaginase

Opeyemi Hannah Akindusoye, Ruth Chinasa Okafor, AO Adekoya, J.B. Minari

Nigéria (code pays fourni par la source)

Comparison analysis of serum interleukin-6 levels and cervical cancer

M. A Adenekan, J.B. Minari, Ayodeji Kayode Adefemi, Gbenga Olorunfemi et autres

We found a significant increase in serum IL-6 levels of 214.9 ng/mL (95% CI: 60.1-369.7,p = 0.007) among women with cervical cancer (CC) compared to their cancer-free counterparts.Objectives: Despite growing evidence of the role of interleukin-6 (IL-6) in CC, studies assessing this …

Nigéria, Afrique du Sud (code pays fourni par la source)

Computational Analysis of Silent Mutation Effects on SARS-CoV-2 RNA-Host RNA-Binding Protein Interactome

Adebayo J. Bello, Onyeka S. Chukwudozie, Olajumoke B. Oladapo, Ayomide O. Omotuyi et autres

RNA-binding proteins (RBPs) play critical roles in host-virus interaction. They facilitate the regulation of viral RNA (vRNA) turnover by recognizing and forming complexes with the vRNA structure via specific RNA motifs-RNA binding domain interaction. However, due to consistent evolving nature of viruses, …

gb, us, Nigéria (code pays fourni par la source)

Impact of different synonymous codon substitution strategies on SARS-CoV-2 nucleocapsid protein expression in Escherichia coli

Adebayo J. Bello, Ayomide O. Omotuyi, Olajumoke B. Oladapo, Adegbayi Adeola Adekunle et autres

Abstract Synonymous codon substitution, a gene engineering approach in synthetic biology, has been effective in improving the codon composition of recombinant genes of interest based on various criteria without altering the amino acid sequence. The SARS-CoV-2 virus nucleocapsid (N) protein is a …

Nigéria, gb (code pays fourni par la source)

Green synthesized silver nanoparticles from Moringa : Potential for preventative treatment of SARS-CoV-2 contaminated water

Adebayo J. Bello, Omorilewa B. Ebunoluwa, Rukayat O. Ayorinde, Nneka Onyepeju et autres

Abstract Biogenic silver nanoparticles have been reported as good antimicrobial candidates. Thus, this study investigated the antiviral activity of silver nanoparticles synthesized against SARS-CoV-2. The silver nanoparticle was biosynthesized using leave extracts of Moringa oleifera (AgNPmo) and characterized using UV-Vis spectroscopy, Fourier …

Nigéria, gb, us (code pays fourni par la source)

Isolation and Characterization of Amylase Produced by CRISPR-Cas 9 Edited LacZ Gene and Unedited Escherichia coli using Potato Peel (Ipomea batata) as Enzyme Source

J.B. Minari, Gift E. Nwosu, Idowu Samuel Dada, Dhikrullah Oluwatope Abdulazeez

Genome editing, particularly using CRISPR-Cas9, is a powerful tool for manipulating genomes, including Escherichia coli. This study aimed to genetically engineer the lacZ gene in E. coli using CRISPR-Cas9 to assess its role in amylase production during submerged fermentation of sweet potato …

CRISPR-CAS9 MEDIATED EDITING OF THE LACZ GENE FOR OPTIMIZING LIPASE PRODUCTION AND CHARACTERIZATION IN ENGINEERED ESCHERICHIA COLI USING POTATO PEEL AS A GLUCOSE SOURCE

J.B. Minari, Idowu Samuel Dada, Dhikrullah Oluwatope Abdulazeez, Gift E. Nwosu

ABSTRACT Improper disposal of potato peels harms the environment, wasting nutrient-rich resources that could be used for beneficial enzyme production like lipase. This study aimed to edit the lacZ gene in Escherichia coli using utilizing the CRISPR Cas9 technology. Both edited and …

Nigéria (code pays fourni par la source)

Cola rostrata K. Schum. constituents induce cytotoxicity through reactive oxygen species generation and mitochondrial membrane depolarisation

Ebenezer Babatunde Ajayi, Bola Oboh, J.B. Minari, Darren W. Sexton et autres

Aim: While the traditional use of Cola rostrata in treating illnesses and diseases has not been reported, the presence of cytotoxic principles has been reported in phylogenetically and biogeographically related species within the Cola genus. This study, therefore, evaluated the cytotoxic potential …

Nigéria, us, gb (code pays fourni par la source)

Status of Expression of GnRH gene in Fed, Starved, and Refed Clarias Gariepinus (African Catfish)

J.B. Minari, Josephine Oshio AYOKHAI, Balikis Olabisi SHOLAJA

Inadequate nutritional status impairs gonadal activities, which may induce reproductive difficulties in Clarias gariepinus. This research was aimed at evaluating the influence of starvation and subsequent refeeding on the expression of GnRH gene in Clarias gariepinus. One hundred and eighty (180) healthy …

Nigéria (code pays fourni par la source)

Characterization of Laccase from the Fungi Fusarium Isolated from Potato Peels Using Carbon and Nitrogen Sources

J.B. Minari, Esther Esesoa Agho, Ekenem Emelumadu, Oloruntimileyin Adeniyi et autres

Laccases (E.C. 1.10.3.2 benzenediol: oxygen oxidoreductase) are an interesting group of N glycosylated multicopper blue oxidase enzymes. They are widely found in fungi, bacteria plants, insects, and lichen. They catalyze the oxidation of various phenolic and non-phenolic compounds, with the concomitant reduction …

Nigéria (code pays fourni par la source)