Abhishek Chatterjee

Repurposing Engineered Aminoacyl-tRNA Synthetases for Proximity-Dependent Protein Labeling through the Release of Aminoacyl-AMP Intermediate

Tarah J. Yared, Elise D. Ficaretta, Lena A. Voss, Conor Loynd et autres

Over the past two decades, engineering efforts have yielded aminoacyl-tRNA synthetases (aaRSs) capable of charging diverse noncanonical amino acids (ncAAs). Here, we report an unexpected and exploitable activity of these engineered aaRSs. In the absence of their cognate tRNA, aaRSs can release …

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Development of m6A Readers for Enhanced Recognition In Vitro via Genetic Code Expansion

Michael Addo, Elise Ficaretta, Yibo Chang, Weiqi Qiu et autres

The YTH domain was recently demonstrated as a promising tool for recognizing and regulating m6A-modified RNAs. However, the deficiency in binding affinity (micromolar range) and selectivity (off-target binding) for m6A of the wild-type YTH domain limit its sensitivity and accuracy for m6A …

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Deep mutational scanning of MaPylRS for ncMs

Chintan Soni, Abhishek Chatterjee

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Deep mutational scanning of MaPylRS for ncMs

Chintan Soni, Abhishek Chatterjee

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Global optimization of suppressor tRNAs in mammalian cells through virus-assisted deep mutation profiling

Abhishek Chatterjee, Zeyi Huang, Rachel L. Huang, Delilah Jewel

Here are the raw .fastq files, processed data files and code for processing .fastq files for publication Global optimization of suppressor tRNAs in mammalian cells through virus-assisted deep mutation profiling.

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Global optimization of suppressor tRNAs in mammalian cells through virus-assisted deep mutation profiling

Abhishek Chatterjee, Zeyi Huang, Rachel L. Huang, Delilah Jewel

Here are the raw .fastq files, processed data files and code for processing .fastq files for publication Global optimization of suppressor tRNAs in mammalian cells through virus-assisted deep mutation profiling.

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B(C 6 F 5 ) 3 -Catalyzed Chemical Tagging of Bioactive N -Alkylamines through β-Amino C(sp 3 )–H Functionalization

Matthew Chapman, Changhang Dai, Yuankai Wang, 赵存元 et autres

We present a late-stage functionalization method for arming bioactive N -alkylamines with functional handles for bioconjugation through the transformations of β-amino C(sp 3 )–H bonds. B(C 6 F 5 ) 3 and a Brønsted base converts N -alkylamines into enamines through sequential …

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Co-Translational Incorporation of ( R )- and ( S )-β 2 -Hydroxyacids In Vivo : Directed Evolution of Efficient Aminoacyl-tRNA Synthetases

Chintan Soni, Meghan A. Pressimone, Malavika A. Nair, Kaitlyn H. Szalay et autres

Expanding the genetic code of living cells with noncanonical monomers (ncMs) relies on engineered aminoacyl-tRNA synthetases (aaRS) and their cognate tRNAs. Conventional aaRS engineering strategies rely on translation-dependent selection systems, limiting their utility for ncMs that are poorly accommodated by the native …

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Co-translational incorporation of (R) and (S) β2 -hydroxyacids in vivo: Directed evolution of efficient aminoacyl-tRNA synthetases

Chintan Soni, Meghan A. Pressimone, Abhishek Chatterjee, Kaitlyn H. Szalay et autres

This is the dataset for the publication in the Journal of American Chemical Society. This dataset includes raw data for PacBio sequencing and Illumina sequencing, as well as intermediate and final analysis files.

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Co-translational incorporation of (R) and (S) β2 -hydroxyacids in vivo: Directed evolution of efficient aminoacyl-tRNA synthetases

Chintan Soni, Meghan A. Pressimone, Abhishek Chatterjee, Kaitlyn H. Szalay et autres

This is the dataset for the publication in the Journal of American Chemical Society. This dataset includes raw data for PacBio sequencing and Illumina sequencing, as well as intermediate and final analysis files.

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An Expanded Toolbox for Versatile Chemical Editing of Adeno‐Associated Virus

Quan Pham, Jake Glicksman, Boyang Han, David Koo et autres

Abstract Site‐specific incorporation of noncanonical amino acids (ncAAs) into the adeno‐associated virus (AAV) capsid offers powerful opportunities to probe and engineer the properties of this leading vector for human gene therapy. However, this approach currently relies almost exclusively on a single azide‐containing …

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An Expanded Toolbox for Versatile Chemical Editing of Adeno‐Associated Virus

Quan Pham, Jake Glicksman, Boyang Han, David Koo et autres

Site-specific incorporation of noncanonical amino acids (ncAAs) into the adeno-associated virus (AAV) capsid offers powerful opportunities to probe and engineer the properties of this leading vector for human gene therapy. However, this approach currently relies almost exclusively on a single azide-containing ncAA, …

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