Ryo Aoki

Inducible rAAV producer cell lines yield vectors equivalent to transient transfection: a physicochemical and biological comparison

Yuki Uchida, Yusuke Mori, Takaaki Kurinomaru, Ryo Aoki et autres

Recombinant adeno-associated virus (rAAV) is one of the most widely used viral vectors in gene therapy. Currently, rAAV is commonly produced by plasmid transient transfection (TT) into host cells; however, the requirement for transfection limits scalability in manufacturing. To overcome this limitation, …

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Data compression for single-pixel imaging using audio codecs

Ryo Aoki, Ryo Kondo, Kenya Sugimoto, Fan Wang et autres

Single-pixel imaging (SPI) reconstructs an object’s image via computational methods using a single-point photodetector and a sequence of mask patterns. A fundamental trade-off exists in SPI between the sampling rate and the fidelity of the reconstructed image: while increasing the number of …

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Binarization for Single-Pixel Imaging Using Error Diffusion with the Maximum and Minimum Values

Kenya Sugimoto, Takashi Shikamura, Keita Miura, Yu Yamazawa et autres

Single-pixel imaging (SPI) differs from conventional imaging techniques because it captures images using a single-pixel sensor, thereby enabling imaging in low-light environments across a wide range of wavelengths. However, one of the major challenges in SPI is that obtaining high-quality reconstructed images …

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城下町村上の初午行事と稲荷信仰

Ryo Aoki

AAV vectors for specific and efficient gene expression in microglia

Ryo Aoki, Ayumu Konno, Nobutake Hosoi, Hayato Kawabata et autres

Microglia are crucial targets for therapeutic interventions in diseases like Alzheimer's and stroke, but efficient gene delivery to these immune cells is challenging. We developed an adeno-associated virus (AAV) vector that achieves specific and efficient gene delivery to microglia. This vector incorporates …

jp (code pays fourni par la source)

Multimodal dataset linking wide‐field calcium imaging to behavior changes in operant lever‐pull task in mice

Masashi Kondo, Keisuke Sehara, Rie Harukuni, Ryo Aoki et autres

The link between comprehensive behavioral measurements during a behavioral task and brain-wide neuronal activity is an essential strategy to better understand the brain dynamics underlying the emergence of behavior changes. To tackle this, we provide an extensive, multimodal dataset that includes 15 …

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A pipeline for mouse brain registration to an atlas after in vivo neurophysiological recordings

Ryo Aoki, Akari Kamigaki, Momoko Daiku, Yasuhiro R. Tanaka

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Enhancement of carbohydrate metabolism by probiotic and prebiotic intake promotes short-chain fatty acid production in the gut microbiome: a randomized, double-blind, placebo-controlled crossover trial

Yuhei Baba, Daisuke Tsuge, Ryo Aoki

Short-chain fatty acids (SCFAs) are thought to be a key factor in the health benefits of prebiotics and probiotics. This study investigated the effect of Bifidobacterium animalis subsp. lactis GCL2505 and inulin (BL + IN) on fecal SCFAs and gut microbial composition …

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A multimodal dataset linking wide-field calcium imaging to behavior changes in mice during an operant lever-pull task

Masashi Kondo, Keisuke Sehara, Rie Harukuni, Ryo Aoki et autres

The BraiDyn-BC (Brain Dynamics underlying emergence of Behavioral Change) Database offers an extensive, multimodal dataset that links wide-field calcium imaging of the mouse neocortex to comprehensive behavioral measurements during an operant conditioning task. This dataset includes 15 sessions spanning two weeks of …

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Development of an AAV Vector System for Highly Specific and Efficient Gene Expression in Microglia

Ryo Aoki, Ayumu Konno, Nobutake Hosoi, Hayato Kawabata et autres

Abstract Microglia play a critical role in diseases such as Alzheimer’s and stroke, making them a significant target for therapeutic intervention. However, due to their immune functions in detecting and combating viral invasion, efficient gene delivery to microglia remains challenging. We achieved …

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Effect of daily ingestion of Bifidobacterium and dietary fiber on vascular endothelial function: a randomized, double-blind, placebo-controlled, parallel-group comparison study

Naoki Azuma, Yasuo Saito, Tomohiko Nishijima, Ryo Aoki et autres

Bifidobacterium animalis subsp. lactis GCL2505 (GCL2505) improves the intestinal microbiota and reduces human visceral fat. This randomized, double-blind, placebo-controlled, parallel-group study was conducted to examine the effects of inulin, a prebiotic dietary fiber, and GCL2505 on vascular endothelial function in healthy subjects …

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Altered gene expression in colon in P2X4 receptor-deficient mice

Kazuki Yoshida, Ryo Aoki, Natsuki Asahina, Yoshiki Morihashi et autres

【Background】The gastrointestinal tract is a barrier between the outside environment and the inside of the organism and is home to an enormous amount of symbiotic bacteria. In addition, various substances derived from bacteria have been shown to influence biological functions. ATP is …

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