Fuyuan Chen

High-efficiency generation of stably transduced monoclonal cell lines via a laser-induced jetting microfluidic platform

Fuyuan Chen, Baojian Zhang, Yue Wang, Hao Peng et autres

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An integrated optofluidic platform enabling label-free single-cell sorting and culture

Baojian Zhang, Fuyuan Chen, Liru Zhao, Mengting Ming et autres

-axis "lift-and-drift" retrieval strategy. By using microwells as hydrodynamic shelters, the platform enables deterministic single-cell sorting and automated collection into standard 96-well plates for subsequent culture while minimizing shear-induced perturbations. The system exhibits robust performance for targets ≥3 µm, demonstrating broad applicability …

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Time-resolved photon counting Fourier-transform micro-spectroscopy enables simultaneous Raman and fluorescence lifetime imaging

Lindong Shang, Xiaodong Bao, Hao Peng, Fuyuan Chen et autres

Abstract To address the current limitations of time-gated Raman spectroscopy, specifically its narrow spectral range and low spectral resolution, and simultaneously acquire Raman and fluorescence life-time images, we have developed a Fourier-transform photon counting spectroscopy platform. A Mach-Zehnder interferometer employing a high …

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Adjustable spot wide-field Raman spectroscopy combined with machine learning for accurate classification of breast cancer cells

Hao Peng, Yu Wang, Xusheng Tang, Lindong Shang et autres

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DDCC: Synergizing Denoising Diffusion Probabilistic Models and Curriculum-Based Complexity Control for Insider Threat Detection

Jiankuo Dong, Zhenjiang Dong, Fuyuan Chen, Xin Yu Hu et autres

Insider threat detection aims to identify malicious activities by employees that may compromise the confidentiality, integrity, or availability of organizational data. Detecting insider threats poses unique challenges compared to external threats, as internal actors often possess authorized system access and familiarity with …

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Association between composite dietary antioxidant index and increased urinary albumin excretion: a population-based study

Shaopeng Li, Yong Wang, Zong-Qi Lin, Fuyuan Chen et autres

Introduction The Albumin-Creatinine Ratio (ACR) is a key biomarker for early kidney disease detection and is predictive of chronic kidney disease (CKD) progression and associated cardiovascular risks. Antioxidant diets, indicated by the Composite Dietary Antioxidant Index (CDAI), may reduce oxidative stress and …

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Automated Laser-Assisted Single-Cell Sorting for Cell Functional and RNA Sequencing

Yuntong Wang, Ying Xue, Huan Wang, Yue Qu et autres

Accurate and efficient sorting of single target cells is crucial for downstream single-cell analysis, such as RNA sequencing, to uncover cellular heterogeneity and functional characteristics. However, conventional single-cell sorting techniques, such as manual micromanipulation or fluorescence-activated cell sorting, do not match current …

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Functionalized microwell laser sorting method for single-cell viable microbial detection

Yuntong Wang, Ying Xue, Xi Hong, Huan Wang et autres

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Siamese network for classification of Raman spectroscopy with inter-instrument variation for biological applications

Xiaodong Bao, Lindong Shang, Fuyuan Chen, Hao Peng et autres

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Application and development of optical-based viscosity measurement technology

Yan Ge, Xingxing Huang, Xusheng Tang, Yuntong Wang et autres

Ultra-fast identification of lactic acid bacteria colonies based on droplet microcavity label-free SERS

Lindong Shang, Fuyuan Chen, Hao Peng, Xiaodong Bao et autres

In this study, we addressed the challenge of excessive fluorescence background in bacterial colony Raman detection and aimed to achieve rapid identification of colonies. To overcome this issue, we employed a combination of droplet microcavity and label-free Surface Enhanced Raman Spectroscopy (SERS) …

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Precision isolation and cultivation of single cells by vortex and flat-top laser ejection

Fuyuan Chen, Kunxiang Liu, Lindong Shang, Yuntong Wang et autres

Single-cell isolation stands as a critical step in single-cell studies, and single-cell ejection technology based on laser induced forward transfer technology (LIFT) is considered one of the most promising methods in this regard for its ability of visible isolating single cell from …

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