Anand S. Mehta

A Protocol for Multivariate Data Visualization and Pseudotime Modeling for Analysis of Disease Trajectories Detected by Mass Spectrometry Imaging

Bryn Gerding, Taylor Hulahan, Laura Spruill, Harrison B. Taylor et autres

Mass spectrometry imaging (MSI) has emerged as a powerful modality for spatially resolved molecular profiling of tumor and stromal compartments; however, computational frameworks for MSI data analysis lag significantly behind those developed for spatial transcriptomics, limiting its translational potential. Here, we introduce …

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Extracellular Matrix Proteomic Signatures Associate with Disease-Free Survival in Later Events of Ductal Carcinoma In Situ or Invasive Breast Cancer

Taylor S. Hulahan, Laura Spruill, Bryn E. Gerding, Jade K. Macdonald et autres

Background: Ductal carcinoma in situ (DCIS) is a noninvasive breast lesion with variable risk of progression to invasive breast cancer (IBC). Current transcription and cell marker investigations suggest ECM decreases in later events but are limited in details of ECM proteomic composition, …

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Strategy for Simultaneous Multiomic Survey of N-Glycomic and Extracellular Matrix Proteome by Mass Spectrometry Imaging

Harrison B. Taylor, Jade K. Macdonald, Walter Myers, Noah Bonnheim et autres

Recent advances in spatially resolved molecular profiling have positioned matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) as a powerful platform for multiomic tissue analyses. However, conventional workflows that sequentially target distinct molecular classes are time- and resource-intensive, requiring repeated sequential sample preparation, …

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Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics

Lauren E Hill, Lyndsay E.A. Young, James W. Dressman, P Angel et autres

Spatial omics has transformed biomedical research by uncovering the molecular characterization of biological systems while preserving spatial context. Among these approaches, mass spectrometry imaging (MSI) provides a label-free, in situ visualization of diverse molecular classes, including metabolites, lipids, proteins, and glycans. Recent …

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Supplementary Figure S7 from N-Linked Fucosylated Glycans Are Biomarkers for Prostate Cancer with a Neuroendocrine and Metastatic Phenotype

Joseph E. Ippolito, Jordan P. Hartig, Kaitlyn Bejar, Hani N. Nakhoul et autres

Figure S7. Example of adenocarcinoma with NE features.

Supplementary Figure S2 from N-Linked Fucosylated Glycans Are Biomarkers for Prostate Cancer with a Neuroendocrine and Metastatic Phenotype

Joseph E. Ippolito, Jordan P. Hartig, Kaitlyn Bejar, Hani N. Nakhoul et autres

Figure S2. Enrichment of glycans in specimens with small cell versus adenocarcinoma histology.

Supplementary Figure S6 from N-Linked Fucosylated Glycans Are Biomarkers for Prostate Cancer with a Neuroendocrine and Metastatic Phenotype

Joseph E. Ippolito, Jordan P. Hartig, Kaitlyn Bejar, Hani N. Nakhoul et autres

Figure S6. Heat map of selected glycans of a mixed GG5 adenocarcinoma and small cell tumor.

Supplementary Figure S8 from N-Linked Fucosylated Glycans Are Biomarkers for Prostate Cancer with a Neuroendocrine and Metastatic Phenotype

Joseph E. Ippolito, Jordan P. Hartig, Kaitlyn Bejar, Hani N. Nakhoul et autres

Figure S8. Fucosylated glycan expression in tumors from prostatectomy specimens do not associate with PSA, grade, or stage.

Supplementary Figure S1 from N-Linked Fucosylated Glycans Are Biomarkers for Prostate Cancer with a Neuroendocrine and Metastatic Phenotype

Joseph E. Ippolito, Jordan P. Hartig, Kaitlyn Bejar, Hani N. Nakhoul et autres

Figure S1. Schematic of fucosylated glycan architecture.

Supplementary Figure S4 from N-Linked Fucosylated Glycans Are Biomarkers for Prostate Cancer with a Neuroendocrine and Metastatic Phenotype

Joseph E. Ippolito, Jordan P. Hartig, Kaitlyn Bejar, Hani N. Nakhoul et autres

Figure S4. Heat map of selected glycans of a GG5 adenocarcinoma.

Supplementary Table S3 from N-Linked Fucosylated Glycans Are Biomarkers for Prostate Cancer with a Neuroendocrine and Metastatic Phenotype

Joseph E. Ippolito, Jordan P. Hartig, Kaitlyn Bejar, Hani N. Nakhoul et autres

Table S3. List of Hyper-fucosylated glycans for prostate cancer analysis

Supplementary Figure S5 from N-Linked Fucosylated Glycans Are Biomarkers for Prostate Cancer with a Neuroendocrine and Metastatic Phenotype

Joseph E. Ippolito, Jordan P. Hartig, Kaitlyn Bejar, Hani N. Nakhoul et autres

Figure S5. Heat map of selected glycans of a metastatic small cell carcinoma to a lymph node.