Siping Huang

Interfacial Energy Balance Governs Initial Cell Spreading Dynamics

Jifeng Ren, Shuhuan Hu, Yi Liu, Siping Huang et autres

Cell spreading is a fundamental process in physiological and pathological contexts, including tissue formation, wound healing, and cancer cell extravasation. Previous studies have examined biophysical mechanisms governing early spreading (around 1-10 min) while biomolecular processes also begin to emerge, yet initial spreading …

hk, cn, it (code pays fourni par la source)

A radiation toxicity biosensing platform based on radioresistant bacteria modified with dr_0423

Xin Cui, Chengpeng Yang, Vincent Li, Siping Huang et autres

Radiation toxicity is important for evaluating potential health risks associated with radioactive substances or environmental radioactivity. Whole-cell biosensors can be applied to quantify radiation toxicity, yet it is technically challenging to identify a radiation-sensitive gene in a radioresistant strain and to maintain …

cn, hk, sg (code pays fourni par la source)

USP32 Promotes Colorectal Carcinoma Progression Through Activating NF ‐ κB Signalling Pathway

Xiaofan Duan, Gaoshaer Yeerkenbieke, Siping Huang, Yanjun Feng

Ubiquitin-specific protease 32 (USP32) plays a key role in cancer progression. However, its functions in colorectal carcinoma (CRC) are still unexplored. In our study, we explored the expression and clinical significance of USP32 in CRC as well as its relationship with the …

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Optimizing the extraction process, analyzing the composition and biological activity of essential oil from Artemisia scoparia using enzyme-assisted steam distillation

Panpan Zhang, Siping Huang, Yuhong Zhang

cn (code pays fourni par la source)

Amplification-free nucleic acids detection with next-generation CRISPR/dx systems

Cia‐Hin Lau, Siping Huang, Haibao Zhu

CRISPR-based diagnostics (CRISPR/Dx) have revolutionized the field of molecular diagnostics. It enables home self-test, field-deployable, and point-of-care testing (POCT). Despite the great potential of CRISPR/Dx in diagnoses of biologically complex diseases, preamplification of the template often is required for the sensitive detection …

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Extended replicative lifespan of primary resting T cells by CRISPR/dCas9-based epigenetic modifiers and transcriptional activators

Siping Huang, Cia‐Hin Lau, Chung Tin, Raymond H. W. Lam

Extension of the replicative lifespan of primary cells can be achieved by activating human telomerase reverse transcriptase (hTERT) to maintain sufficient telomere lengths. In this work, we utilize CRISPR/dCas9-based epigenetic modifiers (p300 histone acetyltransferase and TET1 DNA demethylase) and transcriptional activators (VPH …

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Charge-Powered Electrotaxis for Versatile Droplet Manipulation

Yuankai Jin, Xiaonan Liu, Wanghuai Xu, Pengcheng Sun et autres

Taxis is an instinctive behavior of living organisms to external dangers or benefits. Here, we report a taxis-like behavior associated with liquid droplets on charged substrates in response to the external stimuli, referred to as droplet electrotaxis. Such droplet electrotaxis enables us …

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Developing a multi-sample acoustofluidic device for high-throughput cell aggregation

Renhua Yang, Siping Huang, Yiwen Zhang, Chao Zhang et autres

Abstract Plug-and-play acoustofluidic devices are highly promising for dexterously aggregating microparticles owing to the advantages of being contactless, label-free, and cost-efficient. Despite the extensive progress, existing acoustofluidic devices are largely limited to addressing a single sample per device, lacking the ability to …

hk, cn (code pays fourni par la source)

A Microfluidic Platform Revealing Interactions between Leukocytes and Cancer Cells on Topographic Micropatterns

Xin Cui, Lelin Liu, Jiyu Li, Yi Liu et autres

Immunoassay for detailed analysis of immune-cancer intercellular interactions can achieve more promising diagnosis and treatment strategies for cancers including nasopharyngeal cancer (NPC). In this study, we report a microfluidic live-cell immunoassay integrated with a microtopographic environment to meet the rising demand for …

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Lightweight, ultra-tough, 3D-architected hybrid carbon microlattices

James Utama Surjadi, Yongsen Zhou, Siping Huang, Liqiang Wang et autres

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PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency

Cia‐Hin Lau, Siping Huang, Raymond H. W. Lam, Chung Tin

VP64 is the smallest transactivation domain that can be packaged together with the sgRNA into a single adeno-associated virus (AAV) vector. However, VP64-based CRISPRa often exerts modest activation to the target gene when only one sgRNA is used. Herein, we used PAM-flexible …

hk (code pays fourni par la source)

Developing a Multi-Sample Acoustofluidic Device for High-Throughput Cell Aggregation

Renhua Yang, Siping Huang, Yiwen Zhang, Jingui Qian et autres

hk, cn (code pays fourni par la source)