Danny L. Brower

Differences in Regulation of Drosophila and Vertebrate Integrin Affinity by Talin

Teresa Helsten, Thomas A. Bunch, Hisashi Kato, Jun Yamanouchi et autres

Integrin-mediated cell adhesion is essential for development of multicellular organisms. In worms, flies, and vertebrates, talin forms a physical link between integrin cytoplasmic domains and the actin cytoskeleton. Loss of either integrins or talin leads to similar phenotypes. In vertebrates, talin is …

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Nuclear Localization of the ERK MAP Kinase Mediated byDrosophilaαPS2βPS Integrin and Importin-7

Brian P. James, Thomas A. Bunch, Srinivasan Krishnamoorthy, Lizabeth A. Perkins et autres

The control of gene expression by the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK) requires its translocation into the nucleus. In Drosophila S2 cells nuclear accumulation of diphospho-ERK (dpERK) is greatly reduced by interfering double-stranded RNA against Drosophila importin-7 (DIM-7) or …

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Mutations in the Drosophila αPS2 integrin subunit uncover new features of adhesion site assembly

Danelle Devenport, Thomas A. Bunch, James W. Bloor, Danny L. Brower et autres

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Modulation of Ligand binding by alternative splicing of the αPS2 integrin subunit

Thomas A. Bunch, Timmy Kendall, Kishore Shakalya, Daruka Mahadevan et autres

The Drosophila alphaPS2 integrin subunit is found in two isoforms. alphaPS2C contains 25 residues not found in alphaPS2m8, encoded by the alternative eighth exon. Previously, it was shown that cells expressing alphaPS2C spread more effectively than alphaPS2m8 cells on fragments of the …

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General in Vivo Assay for the Study of Integrin Cell Membrane Receptor Microclustering

Emily A. Smith, Thomas A. Bunch, Danny L. Brower

A method for measuring the microclustering of a class of cell surface receptors called integrins is reported. Integrins are proteins involved in bidirectional signaling across the cell membrane and are important in cell adhesion, growth, and survival. Their activity is regulated by …

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Amino Acid Changes in Drosophila αPS2βPS Integrins That Affect Ligand Affinity

Thomas A. Bunch, Teresa Helsten, Timmy Kendall, Nikhil Shirahatti et autres

We developed a ligand-mimetic antibody Fab fragment specific for Drosophila alphaPS2betaPS integrins to probe the ligand binding affinities of these invertebrate receptors. TWOW-1 was constructed by inserting a fragment of the extracellular matrix protein Tiggrin into the H-CDR3 of the alphavbeta3 ligand-mimetic …

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Identification of Integrin β Subunit Mutations that Alter Heterodimer Function In Situ

Alison L. Jannuzi, Thomas A. Bunch, Robert F. West, Danny L. Brower

We conducted a genetic screen for mutations in myospheroid, the gene encoding the Drosophila betaPS integrin subunit, and identified point mutants in all of the structural domains of the protein. Surprisingly, we find that mutations in very strongly conserved residues will often …

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Analysis of the Drosophila βPS subunit indicates that regulation of integrin activity is a primal function of the C8–C9 loop

Thomas A. Bunch, Steven W. Miller, Danny L. Brower

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Platelets with wings: the maturation of Drosophila integrin biology

Danny L. Brower

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A DM domain protein from a coral, Acropora millepora, homologous to proteins important for sex determination

Steven W. Miller, David C. Hayward, Thomas A. Bunch, David J. Miller et autres

The identification and functional studies of DM domain-containing proteins Doublesex, MAB-3, and DMRT1 indicated that flies, nematodes, and humans share at least some of the molecular mechanisms of sex determination. We identified a gene, AmDM1, from the coral Acropora millepora that encodes …

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Book Review:Imaginal Discs. The Genetic and Cellular Logic of Pattern Formation

Danny L. Brower

Genetic Interaction Between Integrins and moleskin, a Gene Encoding a Drosophila Homolog of Importin-7

Scott Baker, James A. Lorenzen, Steven W. Miller, Thomas A. Bunch et autres

The Drosophila PS1 and PS2 integrins are required to maintain the connection between the dorsal and ventral wing epithelia. If alphaPS subunits are inappropriately expressed during early pupariation, the epithelia separate, causing a wing blister. Two lines of evidence indicate that this …

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