Accès ouvert
2026
article
OpenAlex
Xiaozhe Ding, Xinhong Chen, Philip Boehm, Alba Carretero‐Cerdán et autres
The blood-brain barrier (BBB) presents a challenge for central nervous system (CNS) therapies. Receptor-mediated transcytosis offers a solution, but existing receptor targets are ubiquitous across CNS and peripheral tissues, causing unintended exposure. We identified carbonic anhydrase IV (CA-IV) as a brain-enriched receptor …
us
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Accès ouvert
2026
article
OpenAlex
Jake W. Willows, Lindsey M. Lazor, Gilian Gunsch, Gargi Mishra et autres
Adipose tissue innervation plays a critical role in regulating energy homeostasis and metabolic functions, yet targeted gene delivery to these peripheral nerves has remained challenging. We systematically evaluated both naturally occurring and engineered adeno-associated virus (AAV) capsids for their ability to transduce …
us
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Accès ouvert
2026
preprint
OpenAlex
Mahsa Pourhamzeh, Lara E. Dozier, Amanda Wilpitz, Yixing Du et autres
Abstract Membrane protein trafficking is essential for synaptic growth, maintenance, function, and plasticity, and involves the regulated exocytosis and endocytosis of proteins to and from the pre-and post-synaptic membranes. Defects in the clearance of membrane proteins can lead to the accumulation of …
us
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Accès ouvert
2026
preprint
OpenAlex
Xiaochen Fan, Ronghao Zhou, Brian C. Raftrey, Pamela E. Rios Coronado et autres
Abstract Collateral arteries are natural bypasses that can reroute blood flow around arterial blockages, limiting tissue injury during stroke and coronary artery disease. Despite their clinical effectiveness, therapeutic strategies to stimulate collateral artery growth remain unavailable due to our limited understanding of …
us
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Tyler J. Brittain, Seongmin Jang, Gerard Michael Coughlin, Jonathan D. Hoang et autres
Developing effective vectors for gene therapy requires accurate on-target coverage while minimizing off-target transduction that can lead to adverse events. In mice, the engineered capsid PHP.eB shows enhanced brain transduction, while the further engineered CAP-B10 is also de-targeted from astrocytes and liver. …
us
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Accès ouvert
2026
article
OpenAlex
Yuqing Yan, Jiaxi Lu, Zhe Li, Zuohan Zhao et autres
Abstract Cells constantly change their molecular state in response to internal and external cues 1 . Mapping cellular activity in tissues with spatiotemporal precision is essential for understanding organ physiology, pathology and regenerative processes. Current cell-sensing modalities primarily rely on either end …
us, cn
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Accès ouvert
2026
preprint
OpenAlex
Penny-Shuyi Chen, Declan P Rowley, Mitchell Rudd, Allison Laudano et autres
Abstract Functional optical imaging in nonhuman primates provides an important complement to electrophysiological approaches in neuroscience research, but its broader use has been limited by challenges in achieving large-scale, homogeneous expression of genetically encoded reporters, and imaging accessibility in species with gyrencephalic …
fr, us
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Accès ouvert
2025
article
OpenAlex
Changfan Lin, Xinhong Chen, Jonathan D. Hoang, Fiona Ristic et autres
Gene therapies using natural adeno-associated virus (AAV) serotypes have restricted applications, particularly in the brain, due to their poor targeting and resulting safety concerns. Directed evolution has identified brain-enhanced engineered AAV capsids for various model organisms, but inter-species differences challenge their translation. …
us
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Yuqing Yan, Jian Lü, Zhe Li, Timothy F. Shay et autres
Abstract Mapping cellular activity with high spatiotemporal precision in complex tissues is essential for understanding organ physiology, pathology, and regenerative processes. Here, we introduce G ranularly E xpanding M emory for I ntracellular N arrative I ntegration (GEMINI), an in cellulo recording …
us
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Accès ouvert
2025
preprint
OpenAlex
Tyler J. Brittain, Seongmin Jang, Gerard Michael Coughlin, Bre’Anna H. Barcelona et autres
Abstract Crossing the blood-brain barrier while minimizing liver transduction is a key challenge in developing safe adeno-associated virus (AAV) vectors for treating brain disorders. In mice, the engineered capsid PHP.eB shows enhanced brain transduction, while the further engineered CAP-B10 is also de-targeted …
us
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Changfan Lin, Xinhong Chen, Jonathan D. Hoang, Fiona Ristic et autres
Summary Clinically approved gene therapies based on natural adeno-associated virus (AAV) serotypes have restricted applications, particularly in the brain, due to their poor targeting, high dose requirements, and resulting safety concerns. Directed evolution of enhanced AAV capsids in mice or non-human primates …
us
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Accès ouvert
2024
article
OpenAlex
Eliza C.B. Jaeger, David Vijatovic, Astrid Deryckere, Nikol Zorin et autres
Amphibians, by virtue of their phylogenetic position, provide invaluable insights on nervous system evolution, development, and remodeling. The genetic toolkit for amphibians, however, remains limited. Recombinant adeno-associated viral vectors (AAVs) are a powerful alternative to transgenesis for labeling and manipulating neurons. Although …
us, at, il
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