Tim M. Curtis

Antisense oligonucleotide-mediated disruption of mRNA localisation inhibits angiogenesis

Josy Augustine, Precious O. Owuamalam, Jennifer Nicell, Péter Barabás et autres

mRNA localisation is a critical posttranscriptional mechanism that confers a spatiotemporal dimension to the control of gene expression. Among diverse outcomes, this process can result in compartmentalised protein synthesis and consequently, elicit localised cellular responses. Targeting mRNAs to their destination is often …

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Klebsiella pneumoniae inhibits vasodilation through capsule and T6SS-dependent pathways

Safi Rehman, Joana Sa-Pessoa, Charlotte Buckley, Rachel Lee et autres

Vasodilation is a defence mechanism during inflammation and infection that is regulated by nitric oxide produced by endothelial nitric oxide synthase (eNOS) and the generation of endothelium-dependent hyperpolarization. Several viral infections have been shown to alter vascular biology, but effects of bacterial …

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Klebsiella pneumoniae inhibits vasodilation through capsule and T6SS-dependent pathways

Safi U. Rehman, Joana Sá‐Pessoa, Charlotte Buckley, Rachel L. Lee et autres

Abstract Vasodilation is a defence mechanism during inflammation and infection that is regulated by nitric oxide produced by endothelial nitric oxide synthase (eNOS) and the generation of endothelium-dependent hyperpolarization. Several viral infections have been shown to alter vascular biology, but effects of …

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Pathway-selective mitophagy regulates retinal physiology and neurogenic transitions in Muller glia

Aidan Anderson, Paula Rudzinska, Emer Chang, Dulani Wimalachandra et autres

ABSTRACT Mitochondrial quality control (MQC) is essential for retinal homeostasis, yet how distinct mitophagy pathways are coordinated within specialized retinal cell types remains poorly understood. Here, we show that Müller glia engage distinct mitophagy programmes that are differentially activated across physiological, metabolic …

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Enhancer-directed gene delivery for digit regeneration based on conserved epidermal factors

David A. Brown, Katja K. Koll, Erin Brush, Grant Darner et autres

Abstract Limb loss remains a significant clinical challenge, but regenerative medicine approaches such as gene therapy offer a promising strategy to trigger endogenous regeneration programs. Optimal vector configurations and molecular targets for appendicular skeletal repair are not well defined. Here, we leveraged …

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3D electron microscopy reveals novel ultrastructural changes in the diabetic retinal neurovascular unit

Mona J. Albargothy, Evan P. Troendle, Ross Laws, Péter Barabás et autres

AIMS/HYPOTHESIS: This study used serial block-face scanning electron microscopy (SBF-SEM), a nanoscale imaging technique in x-y-z planes, to investigate 3D ultrastructural changes in the retinal neurovascular unit (NVU) associated with diabetes. We hypothesised that this approach would reveal previously uncharacterised pathological alterations …

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Investigating the validity of structure learning algorithms in identifying risk factors for intervention in patients with diabetes

Sheresh Zahoor, Anthony Costa Constantinou, Tim M. Curtis, Mohammed Hasanuzzaman

Diabetes, a pervasive and enduring health challenge, imposes significant global implications on health, financial healthcare systems, and societal well-being. This study undertakes a comprehensive exploration of various structural learning algorithms to discern causal pathways amongst potential risk factors influencing diabetes progression. This …

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Scavenging acrolein with 2-HDP preserves neurovascular integrity in a rat model of diabetic retinal disease

Josy Augustine, Evan P. Troendle, Thomas Friedel, Eimear M. Byrne et autres

AIMS/HYPOTHESIS: Diabetic retinal disease (DRD) is characterised by progressive neurovascular unit (NVU) dysfunction, often occurring before visible microvascular damage. Our previous studies suggested that the accumulation of acrolein (ACR)-derived protein adducts on retinal Müller cells and neuronal proteins may contribute to NVU …

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Inhibition of glycolysis modulates retinal endothelial cell function and pathological neovascularization

David W. Hughes, Pietro Maria Bertelli, Edoardo Pedrini, Lynsey-Dawn Allen et autres

In many retinopathies, hypoxia stimulates pathogenic neovascularization. The precise impact of hypoxia and how it may drive a switch in retinal microvascular endothelial cell metabolism during active angiogenesis remains ill-defined. This study has sought to understand dynamic shifts in the metabolic profile …

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Klebsiella pneumoniae disrupts vasodilation by targeting eNOS post translational modifications via the type VI secretion system and the capsule polysaccharide

Safi U. Rehman, Joana Sá‐Pessoa, Charlotte Buckley, Rebecca S. Lancaster et autres

SUMMARY Vasodilation is a crucial protective response to inflammation and infection. Endothelial cells control vasodilation through the bioavailability of eNOS-produced nitric oxide (NO), and the generation of endothelium-dependent hyperpolarization (EDH). Here, we demonstrate that Klebsiella pneumoniae , one of the most prevalent …

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The Effect of Moderate Sedation on Intraprocedural Pain in Fluoroscopically Guided Musculoskeletal Procedures: A Real-World Retrospective Cohort Study

Ali Arafat, Tim M. Curtis, Austin Le, Campbell Miller et autres

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The Effectiveness of Cervical Medial Branch Radiofrequency Neurotomy Using a Perpendicular Approach with a Multi-tined Probe: A Single-Arm, Retrospective Cohort Study

Chase Young, Tim M. Curtis, Amanda N. Cooper, Alycia N. Amatto et autres

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