Junko Amemura‐Maekawa

A large outbreak investigation of Legionnaires’ disease associated with a public bath facility in Hiroshima, Japan, using PFGE, SBT, MLVA, and whole-genome sequencing

Takahiro Hiratsuka, Noriko Nakanishi, Hiroko Akita, Kanako Masuda et autres

In 2017, a large outbreak of Legionnaires' disease, involving 58 patients, occurred in a public bath facility in Hiroshima Prefecture, Japan. We analyzed 94 Legionella pneumophila strains isolated from patients and the public bath facility using molecular typing methods, including Pulsed-Field Gel …

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A pluralistic approach to interpreting surveillance data: The likely decline in the occurrence of invasive pneumococcal disease in Japan during the COVID-19 pandemic

Reiko Shimbashi, Yuzo Arima, Koji Yahara, Maki Masutani et autres

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Correlation between bacterial microbiome and Legionella species in water from public bath facilities by 16S rRNA gene amplicon sequencing

Jun-ichi Kanatani, So Fujiyoshi, Junko Isobe, Keiko Kimata et autres

ABSTRACT Public bath facilities are a major source of Legionella infections in Japan. In this study, we performed 16S rRNA gene amplicon sequencing to characterize the bacterial community in bath and shower water from public bath facilities, along with chemical parameters, and …

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Rate of Isolation of Legionella pneumophila in Patients at Kawasaki City, and a Cluster of ST1346

Yusuke Yodotani, Shunkichi HARA, Eiko YUZAWA, Yuka KOZIMA et autres

レジオネラ症はレジオネラ属菌を原因とする感染症であり，国内の患者の報告数は年々増加しているが，感染源不明の事例が多い．本研究では2014年1月から2019年12月までに川崎市で分離されたLegionella pneumophila SG1計28株について感染経路の調査を目的として，Sequence-Based Typing（SBT）法による解析を実施した．その結果ST1346が5株，ST89，ST92及びST142が2株ずつ検出され，残りの17株はそれぞれ異なるSTとなった．最も多く分離されたST1346は5株中4株が2016年11月に短期間に集中して分離されており，ST1346の5株目は11カ月後の2018年10月に分離された．ST1346の5株について全ゲノム解析を行い，pairwise single-nucleotide variant（SNV）の算出を行ったところ，5株間のSNV数は3～6SNVであり，非常に近縁の株であることが明らかとなった．継続的にレジオネラ症患者から菌株を分離しSBT法を実施することで，同一STの集積が確認されSNV解析から同一の感染源に由来することが示唆された．この解析を実施し，疫学調査に反映させることで，集団感染を探知できる可能性がある．

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Complete Genomic Sequence of the Clinical Isolate Legionella pneumophila Serogroup 1 Strain 80-045 from Japan

Masatomo Morita, Nanno Harada, Yuna Shinohara, Miyo Murai et autres

We report the complete genomic sequence of Legionella pneumophila serogroup 1 strain 80-045, isolated from autopsy lung tissue of the first patient diagnosed with Legionnaires' disease in Japan.

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Development of a Multiplex-PCR Serotyping Assay for Characterizing Legionella pneumophila Serogroups Based on the Diversity of Lipopolysaccharide Biosynthetic Loci

Ryota Nakaue, Tian Qin, Masatomo Morita, Hongyu Ren et autres

Legionella pneumophila, which is the main cause of Legionnaires' disease, comprises at least 15 serogroups (SGs). We show here the diversity of lipopolysaccharide biosynthetic loci among serogroups and describe the development of a PCR serotyping assay for 15 SGs based on the …

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Detection of Legionella species, the influence of precipitation on the amount of Legionella DNA, and bacterial microbiome in aerosols from outdoor sites near asphalt roads in Toyama Prefecture, Japan

Jun-ichi Kanatani, Masanori Watahiki, Keiko Kimata, Tomoko Kato et autres

BACKGROUND: Legionellosis is caused by the inhalation of aerosolized water contaminated with Legionella bacteria. In this study, we investigated the prevalence of Legionella species in aerosols collected from outdoor sites near asphalt roads, bathrooms in public bath facilities, and other indoor sites, …

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Investigation of a Legionnaires’ Disease Outbreak in Yamagata City, Japan, 2019, Using Direct Sequence-Based Typing

Junji Seto, Junko Amemura‐Maekawa, Mika Sampei, Kyoko Araki et autres

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Legionella antibiotic susceptibility testing: is it time for international standardization and evidence-based guidance?

Edward Portal, Ghislaine Descours, Christophe Ginévra, M. Mentasti et autres

Legionella pneumophila, a Gram-negative bacillus, is the causative agent of Legionnaire's disease, a form of severe community-acquired pneumonia. Infection can have high morbidity, with a high proportion of patients requiring ICU admission, and up to 10% mortality, which is exacerbated by the …

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Additional file 1 of Detection of Legionella species, the influence of precipitation on the amount of Legionella DNA, and bacterial microbiome in aerosols from outdoor sites near asphalt roads in Toyama Prefecture, Japan

Jun-ichi Kanatani, Masanori Watahiki, Keiko Kimata, Tomoko Kato et autres

Additional file 1.

Detection of Legionella Species and the Influence of Precipitation on the Amount of Legionella DNA Present in Aerosols from Outdoor Sites Near Asphalt Roads in Toyama Prefecture, Japan, with Microbiome Analysis

Jun-ichi Kanatani, Masanori Watahiki, Keiko Kimata, Tomoko Kato et autres

Abstract Background: Legionellosis can be caused by the inhalation of aerosolized water contaminated with Legionella. In this study, we investigated the prevalence of Legionella species in aerosols collected from outdoor sites near asphalt roads, bathrooms in public bath facilities, and other indoor …

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Persistent Legionella contamination of water faucets in a tertiary hospital in Japan

Itaru Nakamura, Junko Amemura‐Maekawa, Fumiaki Kura, Takehito Kobayashi et autres

OBJECTIVE: The feasibility of the decontamination procedure for Legionella pneumophila of water systems in healthcare facilities varies by water purification and disinfection methods in each country. We evaluated the efficacy of feasible decontamination strategies in Japan. METHODS: This study was conducted at …

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