Sebastián Perrone

Molecular mechanisms of transhydrogenase activity and allosteric regulation in eukaryotic type II PHGDH Ser33

Sebastián Perrone, Javier O. Cifuente, Leonardo Mastrella, Alberto Marina et autres

Abstract L-serine is a critical structural constituent of proteins and membrane phospholipids, playing major roles in cell signaling, metabolism and development. L-Serine is synthesized through a conserved de novo pathway starting from the glycolytic intermediate 3-phosphoglycerate (PGA), being oxidized by 3-phosphoglycerate dehydrogenase …

lu, es (code pays fourni par la source)

Clioquinol as a new therapy in epilepsy: From preclinical evidence to a proof‐of‐concept clinical study

Karin Thevissen, Annelii Ny, Daniëlle Copmans, Jana Tits et autres

OBJECTIVE: Drug-resistant epilepsy (DRE) affects >25 million people worldwide and is often associated with neuroinflammation. Increasing evidence links deficiency or malfunctioning of the enzyme phosphoglycerate dehydrogenase (PHGDH), which converts 3-phosphoglycerate to generate serine and the neurotransmitter glycine, with (drug-resistant) epilepsy. Moreover, PHGDH, …

be, nl, au, lu (code pays fourni par la source)

Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells

Adhish S. Walvekar, Marc O. Warmoes, Dean Cheung, Tim Sikora et autres

BACKGROUND: Metabolism is error prone. For instance, the reduced forms of the central metabolic cofactors nicotinamide adenine dinucleotide (NADH) and nicotinamide adenine dinucleotide phosphate (NADPH), can be converted into redox-inactive products, NADHX and NADPHX, through enzymatically catalyzed or spontaneous hydration. The metabolite …

lu, au (code pays fourni par la source)

Clioquinol improves catalytic activity of PHGDH and shows antiseizure efficacy in patients

Karin Thevissen, Annelii Ny, Daniëlle Copmans, Jana Tits et autres

Abstract Drug-resistant epilepsy (DRE) affects over 25 million people worldwide and is associated with neuroinflammation. We identified haloquinolines from a drug repurposing library as potent activators of phosphoglycerate dehydrogenase (PHGDH) enzyme, which converts 3-phosphoglycerate to generate serine and the neurotransmitter glycine, and …

be, us, au, lu (code pays fourni par la source)

Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA

Itxaso Anso, Luis G.M. Basso, Lei Wang, Alberto Marina et autres

. We demonstrate by electron spin resonance spectroscopy and surface plasmon resonance that PatA is an integral membrane acyltransferase tightly anchored to anionic lipid bilayers, using a two-helix structural motif and electrostatic interactions. PatA dictates the acyl chain composition of the glycolipid …

es, br, ca, mx, de, lu, fr, tw (code pays fourni par la source)

Crystal structure of unliganded PatA, a membrane associated acyltransferase from Mycobacterium smegmatis

Itxaso Anso, L. Wang, Alberto Marina, Edgar D. Páez‐Pérez et autres

Conformational Dissection of a Viral Intrinsically Disordered Domain Involved in Cellular Transformation

María G. Noval, Mariana Gallo, Sebastián Perrone, Andrés G. Salvay et autres

Intrinsic disorder is abundant in viral genomes and provides conformational plasticity to its protein products. In order to gain insight into its structure-function relationships, we carried out a comprehensive analysis of structural propensities within the intrinsically disordered N-terminal domain from the human …

ar (code pays fourni par la source)