Jean‐Marc Egly

Pan-inhibition of super-enhancer-driven oncogenic transcription by next-generation synthetic ecteinascidins yields potent anti-cancer activity

Max Cigrang, Julian Obid, Maguelone Nogaret, Léane Seno et autres

The plasticity of cancer cells facilitates their ability to adopt heterogeneous differentiation states, posing a significant challenge to therapeutic interventions. Specific gene expression programs, driven in part by super-enhancers (SEs), underlie cancer cell states. Here we successfully inhibit SE-driven transcription in phenotypically …

fr, us, es, tw (code pays fourni par la source)

Unveiling the Mechanism of Lurbinectedin’s Action and Its Potential in Combination Therapies in Small Cell Lung Cancer

Antonio Calles, Emiliano Calvo, Gema Santamaría Núñez, Federico Costanzo et autres

Lurbinectedin is a selective inhibitor of oncogenic transcription approved for the treatment of adult patients with metastatic small cell lung cancer with disease progression on or after platinum-based chemotherapy. Preclinical data provide evidence for lurbinectedin exerting its actions in a unique manner …

es, ch, us, fr, tw (code pays fourni par la source)

CS proteins and ubiquitination: orchestrating DNA repair with transcription and cell division

Federico Costanzo, Elena Paccosi, Luca Proietti‐De‐Santis, Jean‐Marc Egly

fr, ch, it, tw (code pays fourni par la source)

Novel synthetic ecteinascidins exhibit potent anti-melanoma activity by suppressing super-enhancer-driven oncogenic transcription

Max Cigrang, Julian Obid, Maguelone Nogaret, Léane Seno et autres

Abstract The dynamic cellular transitions exhibited by skin cutaneous melanoma (SKCM) cells present a significant challenge to current therapeutic approaches, emphasizing the critical need for innovative treatments. Lurbinectedin, a marine-derived compound belonging to the ecteinascidin family, has recently gained approval for the …

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Promoters of ASCL1‐ and NEUROD1‐dependent genes are specific targets of lurbinectedin in SCLC cells

Federico Costanzo, Marta Martínez, Gema Santamaría Núñez, Juan Ignacio Díaz‐Hernandéz et autres

Small-Cell Lung Cancer (SCLC) is an aggressive neuroendocrine malignancy with a poor prognosis. Here, we focus on the neuroendocrine SCLC subtypes, SCLC-A and SCLC-N, whose transcription addiction was driven by ASCL1 and NEUROD1 transcription factors which target E-box motifs to activate up …

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CDK7 and MITF repress a transcription program involved in survival and drug tolerance in melanoma

Pietro Berico, Max Cigrang, Guillaume Davidson, Cathy Braun et autres

fr (code pays fourni par la source)

The Cockayne syndrome group A and B proteins are part of a ubiquitin–proteasome degradation complex regulating cell division

Elena Paccosi, Federico Costanzo, Michele Costantino, Alessio Balzerano et autres

Cytokinesis is monitored by a molecular machinery that promotes the degradation of the intercellular bridge, a transient protein structure connecting the two daughter cells. Here, we found that CSA and CSB, primarily defined as DNA repair factors, are located at the midbody, …

it, fr, tw (code pays fourni par la source)

DNA repair complex licenses acetylation of H2A.Z.1 by KAT2A during transcription

Maryssa Semer, Baptiste Bidon, A. Larnicol, Gizem Caliskan et autres

fr, tr (code pays fourni par la source)

The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH

Laura Radu, Elisabeth Schoenwetter, Cathy Braun, Julien Marcoux et autres

The general transcription factor IIH (TFIIH) is a multi-protein complex and its 10 subunits are engaged in an intricate protein-protein interaction network critical for the regulation of its transcription and DNA repair activities that are so far little understood on a molecular …

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Abstract 3039: Lurbinectedin specifically targets transcription in cancer cells, triggering DNA breaks and degradation of phosphorylated Pol II

Gema Santamaría-Núñez, Carlos M. Genes-Robles, Juan Fernando Martínez-Leal, Carlos María Galmarini et autres

Abstract Cancer cells are characterized for their avid demand for active transcription, which reaches the level of a real addiction in solid tumors as small cell lung cancer (SCLC) or triple-negative breast cancer. Pharmacological modulation of transcription may thus provide a therapeutic …

es, fr (code pays fourni par la source)

Purification and functional characterization of a cellular transcription factor that binds to an enhancer element within the adenovirus early EIla promoter (affinity chromatography/band-shift/gene regulation/HeLa cells/in vitro transcription)

Pierre Jalinot, Marguerite Wintzerith, Mireille Gaire, Charlotte Hauss et autres

The adenovirus EIa-inducible early EIIa (EIIaE) promoter is comprised of several sequence elements essential for constitutive and Induced expression. We report here the purification of the host-cell factor that interacts with the major upstream element of this promoter, extending between positions -90 …

Crystal Structure of the Rad3/XPD Regulatory Domain of Ssl1/p44

Jin Seok Kim, Charlotte Saint-André, Hye Seong Lim, Cheol‐Sang Hwang et autres

The Ssl1/p44 subunit is a core component of the yeast/mammalian general transcription factor TFIIH, which is involved in transcription and DNA repair. Ssl1/p44 binds to and stimulates the Rad3/XPD helicase activity of TFIIH. To understand the helicase stimulatory mechanism of Ssl1/p44, we …

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