R. Tillie

Atherosclerotic plaque fibroblasts derive from adventitial and medial Pdgfra -lineage-positive cells and predominantly maintain fibroblast identity

Guanliang Li, S. Asselberghs, Baixue Yu, Cong Feng et autres

AIMS: Fibroblasts are mesenchymal cells in the healthy vascular adventitia. In atherosclerosis, single-cell sequencing datasets suggest fibroblasts are abundant in plaques. However, their identity, origin, and fate during plaque progression remain unclear, which we aim to unravel here. APPROACH AND RESULTS: To …

nl, cn, de, ca, us, it, gb (code pays fourni par la source)

Myeloid PHD2 Conditional Knockout Improves Intraplaque Angiogenesis and Vascular Remodeling in a Murine Model of Venous Bypass Grafting

Thijs J. Sluiter, R. Tillie, Alwin de Jong, J Bruijn et autres

BACKGROUND: Intraplaque angiogenesis occurs in response to atherosclerotic plaque hypoxia, which is driven mainly by highly metabolically active macrophages. Improving plaque oxygenation by increasing macrophage hypoxic signaling, thus stimulating intraplaque angiogenesis, could restore cellular function and neovessel maturation, and decrease plaque formation. …

nl, gb, us (code pays fourni par la source)

Human and murine single-cell RNA-sequencing reveal fibroblast heterogeneity in healthy and diseased vasculature and differential regulation by ageing and serum cholesterol

Kim van Kuijk, Ian R McCracken, R. Tillie, S. Asselberghs et autres

nl, gb, de, us (code pays fourni par la source)

Partial myeloid inhibition of key glycolytic enzyme PFKFB3 increases hepatic steatosis and inflammation, but does not affect atherosclerosis

R. Tillie, Joost D. de Bruijn, Ross Dobie, Javier Perales-Patón et autres

nl, gb, de, us, be (code pays fourni par la source)

Human and murine fibroblast single-cell transcriptomics reveals fibroblast clusters are differentially affected by ageing and serum cholesterol

Kim van Kuijk, Ian R McCracken, R. Tillie, S. Asselberghs et autres

AIMS: Specific fibroblast markers and in-depth heterogeneity analysis are currently lacking, hindering functional studies in cardiovascular diseases (CVDs). Here, we established cell-type markers and heterogeneity in murine and human arteries and studied the adventitial fibroblast response to CVD and its risk factors …

us, nl, de, gb (code pays fourni par la source)

Partial Inhibition of the 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase-3 (PFKFB3) Enzyme in Myeloid Cells Does Not Affect Atherosclerosis

R. Tillie, Jenny de Bruijn, Javier Perales-Patón, Lieve Temmerman et autres

Background The protein 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3) is a key stimulator of glycolytic flux. Systemic, partial PFKFB3 inhibition previously decreased total plaque burden and increased plaque stability. However, it is unclear which cell type conferred these positive effects. Myeloid cells play an important role …

nl, de, us, cn, dk, be, gb (code pays fourni par la source)

Single cell sequencing reveals fibroblast heterogeneity in healthy and diseased vasculature

Kim van Kuijk, Ian R McCracken, R. Tillie, Ruud Wichers Schreur et autres

nl, gb, de, us (code pays fourni par la source)

A Switch from Cell-Associated to Soluble PDGF-B Protects against Atherosclerosis, despite Driving Extramedullary Hematopoiesis

R. Tillie, Thomas Theelen, Kim van Kuijk, Lieve Temmerman et autres

Platelet-derived growth factor B (PDGF-B) is a mitogenic, migratory and survival factor. Cell-associated PDGF-B recruits stabilizing pericytes towards blood vessels through retention in extracellular matrix. We hypothesized that the genetic ablation of cell-associated PDGF-B by retention motif deletion would reduce the local …

nl, se, de, gb (code pays fourni par la source)

Fibroblasts in atherosclerosis: heterogeneous and plastic participants

R. Tillie, Kim van Kuijk, Judith C. Sluimer

PURPOSE OF REVIEW: Fibroblasts are very heterogeneous and plastic cells in the vasculature. A growing interest in fibroblasts in healthy and atherosclerotic vasculature is observed, next to macrophages, endothelial cells, and smooth muscle cells (SMCs). In this review, we discuss fibroblast presence, …

nl, gb (code pays fourni par la source)

Pdgf-B Retention Motif Deletion Has Mural Cell-Independent Effects Including Leukocytosis And Increased Murine Atherosclerotic Plaque Stability Due To Macrophage Apoptosis And Decreased Mmp Activity

R. Tillie, Thomas Theelen, Kim van Kuijk, Marion J. Gijbels et autres

nl, se (code pays fourni par la source)