William Derval Aiken

CKD Prevalence and Associated Factors in Jamaica

Lori-Ann M. Fisher, Trevor S. Ferguson, Kern D. Rocke, Natalie G Guthrie-Dixon et autres

Introduction: Jamaica has a high attributable burden of chronic kidney disease (CKD) but no population-based prevalence estimates. We aimed to estimate the prevalence of CKD and explore associated factors. Methods: , using the CKD Epidemiology Collaboration 2021 or Schwartz-Lyon equations, and/or urine …

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Neighborhood disorder and depressive symptoms in Jamaican adults: the mediating roles of neighborhood crime and safety problems and collective efficacy

Colette Andrea Cunningham-Myrie, Tina Moore, Jovan Wiggan, NO Younger-Coleman et autres

Background: Neighborhood disorder has been found to be associated with worse mental health outcomes, such as depression. This study examined the association between perceived neighborhood disorder on depressive symptoms in a nationally representative sample of Jamaican adults, and whether any association was …

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Chronic Kidney Disease in Jamaica: Estimated Prevalence and Associated Risk Factors from the Jamaica Health and Lifestyle Survey III

Lori-Ann M Fisher, Trevor S. Ferguson, Kern D. Rocke, Natalie G Gurthrie-Dixon et autres

Abstract Introduction Jamaica has a high attributable burden of chronic kidney disease (CKD) but no population-based prevalence estimates. We aimed to estimate the prevalence of CKD and explore associated factors. Methods A secondary analysis of data from Jamaican residents aged ≥15 years …

Abstract 1824: Hexadecanoic acid mediated cell cycle arrest involving inhibition of cell migration

Sasha-gay A. Wright, William Derval Aiken, Helen N. Asemota, Kimberly A. Foster et autres

Abstract The Caribbean region accounts for the highest incidence and mortality rates of prostate cancer in the world. This warrants the need for targeted therapy as currently the most prevalent and effective option include chemotherapeutic drugs. Previous studies showed that an enriched …

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Utility of formalin-fixed, paraffin-embedded prostate biospecimens from low-resource settings for use in next-generation sequencing studies in African-descent populations

Ernest T. Kaninjing, Kayode Adebamiji Adeniji, Andrew Gachii, Paul Jibrin et autres

Background Men of African ancestry experience higher burden from prostate cancer compared to men of other ancestral backgrounds. Limitations in the availability of high-quality biospecimens hinder the inclusion of this population in genetic studies of prostate cancer. The use of formalin-fixed paraffin-embedded …

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Data from Expanding the Prostate Cancer Cell Line Repertoire with ACRJ-PC28, an AR-negative Neuroendocrine Cell Line Derived From an African-Caribbean Patient

Henkel Valentine, William Derval Aiken, Belinda F. Morrison, Ziran Zhao et autres

Prostate cancer cell lines from diverse backgrounds are important to addressing disparities in prostate cancer incidence and mortality rates among Black men. ACRJ-PC28 was developed from a transrectal needle biopsy and established via inactivation of the CDKN2A locus and simultaneous expression of …

Supplemental table ST1: Sample collection and culture conditions from Expanding the Prostate Cancer Cell Line Repertoire with ACRJ-PC28, an AR-negative Neuroendocrine Cell Line Derived From an African-Caribbean Patient

Henkel Valentine, William Derval Aiken, Belinda F. Morrison, Ziran Zhao et autres

In these prior attempts, culture conditions such as media, extra-cellular matrices and culture flasks were varied to determine the optimal culture conditions for generation of prostate cancer explants.

Supplemental Table ST2: from Expanding the Prostate Cancer Cell Line Repertoire with ACRJ-PC28, an AR-negative Neuroendocrine Cell Line Derived From an African-Caribbean Patient

Henkel Valentine, William Derval Aiken, Belinda F. Morrison, Ziran Zhao et autres

Supplemental Table 2: A search in the database of global cell repositories ATCC and ECACC revealed that approximately 40% of available prostate derived lines were immortalized via an exogenous source.

Supplemental Figure SF4: The cytotoxic effect of a Cannabis extract, THC_EX3 on the viability of ACRJ-CP28 and PC3 cell line. from Expanding the Prostate Cancer Cell Line Repertoire with ACRJ-PC28, an AR-negative Neuroendocrine Cell Line Derived From an African-Caribbean Patient

Henkel Valentine, William Derval Aiken, Belinda F. Morrison, Ziran Zhao et autres

Cell viability was determined using the MTS assay and OD readings were taken a 409nm. Plots were generated in Microsoft Excel and each data point represents the average of three replicates. Experiments shown are representative of two independent experiments.

Supplementary Table ST3 from Expanding the Prostate Cancer Cell Line Repertoire with ACRJ-PC28, an AR-negative Neuroendocrine Cell Line Derived From an African-Caribbean Patient

Henkel Valentine, William Derval Aiken, Belinda F. Morrison, Ziran Zhao et autres

All Exonic Variants which are present in ACRJ-PC28. SIFT, Polyphen2, LRT, MutationTaster, MutationAssessor, and CADD were used to predict variant effect. Pop_Freq_max is the maximum population frequency of the variant in 1000Genome Project (Aug 2015), Exome Sequence Project (ESP6500) and Genome Aggregation …

Supplemental Figure SF1: STR profiling for ACRJ-PC28 cells. from Expanding the Prostate Cancer Cell Line Repertoire with ACRJ-PC28, an AR-negative Neuroendocrine Cell Line Derived From an African-Caribbean Patient

Henkel Valentine, William Derval Aiken, Belinda F. Morrison, Ziran Zhao et autres

STR profile of ACRJ-PC28 generated by IDEXX using PC indicates the novelty of the cell line (A) as the cell line senctic profile was compared to the profiles of other cell lines in the DSMZ STR database and did not match anv …

Supplemental Figure SF3: IHC staining on ACRJ-PC28 cells compared to positive controls. from Expanding the Prostate Cancer Cell Line Repertoire with ACRJ-PC28, an AR-negative Neuroendocrine Cell Line Derived From an African-Caribbean Patient

Henkel Valentine, William Derval Aiken, Belinda F. Morrison, Ziran Zhao et autres

IHC staining on ACRJ-PC28 cells compared to positive controls.