Accès ouvert
2026
article
OpenAlex
Jisun Jung, Alec Wehmeier, Zili Xie, David Horton et autres
Inflammation results in increased gastrointestinal (GI) motility and diarrhea, but the mechanism remains unclear. Contrary to expectations, we found that neuronal responses to cytokine inhibited GI motility, as Nav1.8-Cre-mediated neuronal deletion of Jak1, a cytokine signaling intermediate, markedly increased GI motility at …
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Accès ouvert
2021
article
OpenAlex
Emilie V. Russler-Germain, Jisun Jung, Aidan T. Miller, Shannon Young et autres
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Accès ouvert
2020
article
OpenAlex
Wesley A. Wierson, Jordan M. Welker, Maira P. Almeida, Carla M. Mann et autres
Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our …
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Accès ouvert
2020
peer-review
OpenAlex
Wesley A. Wierson, Jordan M. Welker, Maira P. Almeida, Carla M. Mann et autres
Short homology arms exposed by CRISPR/Cas9 cleavage can target integration at genomic CRISPR/Cas9 cut sites at high frequencies with reproducible precision using pGTag vectors in zebrafish and mammalian cells.
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Accès ouvert
2018
preprint
OpenAlex
Wesley A. Wierson, Jordan M. Welker, Maira P. Almeida, Carla M. Mann et autres
Abstract Choices for genome engineering and integration involve high efficiency with little or no target specificity or high specificity with low activity. Here, we describe a targeted integration strategy, called GeneWeld, and a vector series for gene tagging, pGTag ( p lasmids …
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