Alex McDougall

Spatiotemporal regulation of LGN/NuMA and astral microtubules generate mirror symmetric spindle rotations

Janet Chenevert, Anne Rosfelter, Daniel González-Suárez, Silvia Caballero-Mancebo et autres

Abstract The positioning of the mitotic spindle controls the size, content, and position of daughter cells within embryos and tissues. A major spindle positioning mechanism is “cortical pulling” whereby the membrane-bound complex LGN/NuMA/Dynein captures astral microtubules and pulls centrosomes toward the cell …

fr, jp (code pays fourni par la source)

The extraordinary robustness of mitotic spindle assembly to microtubule dynamics plasticity revealed by end-binding proteins and tubulin tagging

Morgane L. V. Robert, Aurélien Perrier, Janet Chenevert, Layla El Mossadeq et autres

Abstract Microtubules are dynamic, conserved cytoskeletal filaments that are essential for all eukaryotic cells. Microtubule dynamic properties are primarily characterized by measuring how fast they grow and shrink (growth and shrinkage rates) and how often they switch between assembly and disassembly (catastrophe …

fr, us (code pays fourni par la source)

Tug-of-war between cortical and cytoplasmic forces shaping planar 4-cell stage embryos

Silvia Caballero-Mancebo, Daniel Gonzalez Suarez, Janet M. Chenevert, Sameh Ben-Aicha et autres

ABSTRACT Early embryonic cleavages often follow conserved geometric rules, resulting in species-specific cleavage patterns. How these rules are mechanistically implemented, however, varies widely across species and remains poorly understood. Here, using quantitative 3D live imaging, mechanical and biochemical manipulations, we dissect the …

fr (code pays fourni par la source)

Marine Model Organisms for Mechanobiology Studies

Silvia Caballero-Mancebo, Daniel Gonzalez Suarez, Janet M. Chenevert, Alex McDougall et autres

fr (code pays fourni par la source)

Self-Supervised Z-Slice Augmentation for 3D Bio-Imaging via Knowledge Distillation.

Alessandro Pasqui, Sajjad Mahdavi, Benoît Vianay, Alexandra Colin et autres

Three-dimensional biological microscopy has significantly advanced our understanding of complex biological structures. However, limitations due to microscopy techniques, sample properties or phototoxicity often result in poor z-resolution, hindering accurate cellular measurements. Here, we introduce ZAugNet, a fast, accurate, and self-supervised deep learning …

fr, us (code pays fourni par la source)

Self-Supervised Z-Slice Augmentation for 3D Bio-Imaging via Knowledge Distillation

Alessandro Pasqui, Sajjad Mahdavi, Benoît Vianay, Alexandra Colin et autres

Three-dimensional biological microscopy has significantly advanced our understanding of complex biological structures. However, limitations due to microscopy techniques, sample properties or phototoxicity often result in poor z-resolution, hindering accurate cellular measurements. Here, we introduce ZAugNet, a fast, accurate, and self-supervised deep learning …

fr (code pays fourni par la source)

Reduction of cortical pulling at mitotic entry facilitates aster centration

Anne Rosfelter, Ghislain de Labbey, Janet M. Chenevert, Rémi Dumollard et autres

Equal cell division relies upon astral microtubule-based centering mechanisms, yet how the interplay between mitotic entry, cortical force generation and long astral microtubules leads to symmetric cell division is not resolved. We report that a cortically located sperm aster displaying long astral …

fr, us (code pays fourni par la source)

Measuring Mitotic Spindle and Microtubule Dynamics in Marine Embryos and Non-model Organisms

Janet M. Chenevert, Morgane L. V. Robert, Jérémy Sallé, Sébastien Cacchia et autres

fr (code pays fourni par la source)

Author Reply to Peer Reviews of Reduction of cortical pulling at mitotic entry facilitates aster centration

Anne Rosfelter, Ghislain de Labbey, Janet M. Chenevert, Rémi Dumollard et autres

fr, us (code pays fourni par la source)

Embryo mechanics cartography: inference of 3D force atlases from fluorescence microscopy

Sacha Ichbiah, Fabrice Delbary, Alex McDougall, Rémi Dumollard et autres

Tissue morphogenesis results from a tight interplay between gene expression, biochemical signaling and mechanics. Although sequencing methods allow the generation of cell-resolved spatiotemporal maps of gene expression, creating similar maps of cell mechanics in three-dimensional (3D) developing tissues has remained a real …

fr (code pays fourni par la source)

Embryo mechanics cartography: inference of 3D force atlases from fluorescence microscopy

Sacha Ichbiah, Fabrice Delbary, Alex McDougall, Rémi Dumollard et autres

Abstract The morphogenesis of tissues and embryos results from a tight interplay between gene expression, biochemical signaling and mechanics. Although sequencing methods allow the generation of cell-resolved spatio-temporal maps of gene expression in developing tissues, creating similar maps of cell mechanics in …

fr (code pays fourni par la source)

Reduction of cortical pulling at mitotic entry facilitates aster centration

Anne Rosfelter, Ghislain de Labbey, Janet M. Chenevert, Rémi Dumollard et autres

Abstract Although it has been studied for more than a century, the question of how one cell divides into two equal parts is still not fully resolved. Zygotes have provided much of the mechanistic insight into how the mitotic apparatus finds the …

fr, us (code pays fourni par la source)