Kaja Kiiver

Tetravalent antibodies are more potent and efficacious erythropoiesis‐stimulating agents than erythropoietin in vivo

Jarrett Adams, Levi L. Blazer, Jacky Chung, Minoo Karimi et autres

Recent studies have shown that tetravalent antibodies are potent and efficacious agonists of the erythropoietin (EPO) receptor (EPOR) both in vitro and in vivo. To identify antibody-based erythropoiesis-stimulating agents (ESAs) with therapeutic potential, we evaluated various tetravalent antibody formats for EPOR agonism …

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RIG-I and MDA-5 Detection of Viral RNA-dependent RNA Polymerase Activity Restricts Positive-Strand RNA Virus Replication

Andrei Nikonov, Tarmo Mölder, Rein Sikut, Kaja Kiiver et autres

Type I interferons (IFN) are important for antiviral responses. Melanoma differentiation-associated gene 5 (MDA-5) and retinoic acid-induced gene I (RIG-I) proteins detect cytosolic double-stranded RNA (dsRNA) or 5'-triphosphate (5'-ppp) RNA and mediate IFN production. Cytosolic 5'-ppp RNA and dsRNA are generated during …

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P19-30. GTU® as beneficial HIV DNA vaccine vector system

Andres Männik, Urve Toots, Rein Sikut, Katrin Kaldma et autres

The general objective has been to generate the genetic vaccine vector that assures induction of strong and protective T-cell and B-cell immune responses against the HIV1. We have developed Gene Transport Unit (GTU ) plasmid vector system, which uses bovine papillomavirus type …

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Semliki Forest virus based vectors and cell lines for studying the replication and interactions of alphaviruses and hepaciviruses

Kaja Kiiver

Semliki Forest virus (SFV; genus Alphavirus) has provided basic knowledge on the molecular biology of alpha­viruses, but also elucidated several general viral and cellular processes. The simple genomic organization of alphaviruses and their efficient propa­gation in cell culture have made alphavirus-based vectors …

Neurons and oligodendrocytes in the mouse brain differ in their ability to replicate Semliki Forest virus

Rennos Fragkoudis, Nele Tamberg, Ricky W. C. Siu, Kaja Kiiver et autres

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Properties of non-structural protein 1 of Semliki Forest virus and its interference with virus replication

Kaja Kiiver, Ingrid Tagen, Eva Žusinaite, Nele Tamberg et autres

Semliki Forest virus (SFV) non-structural protein 1 (nsP1) is a major component of the virus replicase complex. It has previously been studied in cells infected with virus or using transient or stable expression systems. To extend these studies, tetracycline-inducible stable cell lines …

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Novel vectors expressing anti-apoptotic protein Bcl-2 to study cell death in Semliki Forest virus-infected cells

Kaja Kiiver, Andres Merits, Inga Sarand

Semliki Forest virus (SFV, Alphavirus) induce rapid shut down of host cell protein synthesis and apoptotic death of infected vertebrate cells. Data on alphavirus-induced apoptosis are controversial. In this study, the anti-apoptotic bcl-2 gene was placed under the control of duplicated subgenomic …

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Mutations at the palmitoylation site of non-structural protein nsP1 of Semliki Forest virus attenuate virus replication and cause accumulation of compensatory mutations

Eva Žusinaite, Kairit Tints, Kaja Kiiver, Pirjo Spuul et autres

The replicase of Semliki Forest virus (SFV) consists of four non-structural proteins, designated nsP1-4, and is bound to cellular membranes via an amphipathic peptide and palmitoylated cysteine residues of nsP1. It was found that mutations preventing nsP1 palmitoylation also attenuated virus replication. …

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Complex formation between hepatitis C virus NS2 and NS3 proteins

Kaja Kiiver, Andres Merits, Mart Ustav, Eva Žusinaite

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Hepatitis C virus genotypes in Estonia

Eva Žusinaite, Tõnu Krispin, Elve Raukas, Kaja Kiiver et autres

Distribution of hepatitis C virus (HCV) geno(sub)types among 215 Estonian patients hospitalized with acute or chronic hepatitis and with HCV RNA-positive sera was investigated. For genotyping, both multiplex PCR with subtype-specific primers of the core region and RFLP analysis of cDNA of …

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