Accès ouvert
2026
preprint
OpenAlex
Sharad Sarak, Hong Yang, Colin T. Pierce, Panhavuth Tan et autres
Abstract Active-site redesign frequently yields modest improvements because residues controlling physical steps like substrate binding and product release lie outside the active site. Efficient catalysis requires a cooperative catalytic network of residues that support both the chemical and physical steps of catalysis. …
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Accès ouvert
2025
article
OpenAlex
Colin T. Pierce, Panhavuth Tan, Lauren R. Greenberg, Meghan E. Walsh et autres
Hydroxynitrile lyase from Hevea brasiliensis ( Hb HNL) and the esterase SABP2 from Nicotiana tabacum share the α/β-hydrolase fold, a Ser–His–Asp catalytic triad and 44% sequence identity, yet catalyze different reactions. Prior studies showed that three active-site substitutions in Hb HNL conferred …
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Accès ouvert
2025
preprint
OpenAlex
Colin T. Pierce, Panhavuth Tan, Lauren R. Greenberg, Meghan E. Walsh et autres
Abstract The α/β-hydrolase fold family contains mostly esterases but includes other enzymes such as hydroxynitrile lyase from Hevea brasiliensis (rubber tree, Hb HNL). Hb HNL shares 44% sequence identity and a Ser-His-Asp catalytic triad with esterase SABP2 (salicylic acid binding protein 2 …
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Accès ouvert
2018
conference-abstract
OpenAlex
Kuniko Kadoya, P. Nido, Elizabeth T Makino, A. Nguyen et autres
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