Accès ouvert
2025
article
OpenAlex
Emilie Marhuenda, Ioannis Xanthis, Poppy O. Smith, Aishwarya Prakash et autres
During heart disease, the cardiac extracellular matrix (ECM) undergoes a structural and mechanical transformation. Cardiomyocytes sense the mechanical properties of their environment, leading to phenotypic remodeling. A critical component of the ECM mechanosensing machinery, including the protein talin, is organized at the …
fr, gb, it
(code pays fourni par la source)
Accès ouvert
2025
conference-abstract
OpenAlex
Emilie Marhuenda, Ioannis Xanthis, Aishwarya Prakash, Till Kallem et autres
Introduction During heart disease, the cardiac extracellular matrix (ECM) mechanically transforms, resulting in the stiffening of the ECM. Cardiomyocytes sense this ECM change, leading to phenotypic remodelling. A key part of the cardiomyocyte mechanosensing machinery is organised at the costamere; integrins and …
fr, gb, it
(code pays fourni par la source)
Accès ouvert
2023
preprint
OpenAlex
Emilie Marhuenda, Ioannis Xanthis, Pragati Pandey, Amar J. Azad et autres
Abstract Mechanical properties are cues for many biological processes in health or disease. Likewise, in the heart it is becoming clearer that mechanical signals are critically involved in the disease progression. Cardiomyocytes sense the mechanical properties of their environment at costameres through …
gb, it
(code pays fourni par la source)
Accès ouvert
2021
article
OpenAlex
Ah-Lai Law, Shamsinar Jalal, Tommy L. Pallett, Fuad Mosis et autres
Cell migration is important for development and its aberrant regulation contributes to many diseases. The Scar/WAVE complex is essential for Arp2/3 mediated lamellipodia formation during mesenchymal cell migration and several coinciding signals activate it. However, so far, no direct negative regulators are …
gb, de
(code pays fourni par la source)
Accès ouvert
2020
preprint
OpenAlex
Ah-Lai Law, Shamsinar Jalal, Tommy L. Pallett, Fuad Mosis et autres
Abstract Cell migration is important for development and its aberrant regulation contributes to many diseases. The Scar/WAVE complex is essential for Arp2/3 mediated lamellipodia formation during mesenchymal cell migration and several coinciding signals activate it. However, so far, no direct negative regulators …
gb, de
(code pays fourni par la source)
Accès ouvert
2020
article
OpenAlex
Ida Emilie Steinmark, Pei‐Hua Chung, Robert M. Ziolek, Bethan Cornell et autres
Understanding viscosity in complex environments remains a largely unanswered question despite its importance in determining reaction rates in vivo. Here, time-resolved fluorescence anisotropy imaging (TR-FAIM) is combined with fluorescent molecular rotors (FMRs) to simultaneously determine two non-equivalent viscosity-related parameters in complex heterogeneous …
gb
(code pays fourni par la source)
Accès ouvert
2020
article
OpenAlex
James A. Levitt, Simon P. Poland, Nikola Krstajić, Karin Pfisterer et autres
Fluorescence lifetime imaging (FLIM) is a quantitative, intensity-independent microscopical method for measurement of diverse biochemical and physical properties in cell biology. It is a highly effective method for measurements of Förster resonance energy transfer (FRET), and for quantification of protein-protein interactions in …
gb
(code pays fourni par la source)
2020
conference-paper
OpenAlex
Simon Ameer‐Beg, Simon P. Poland, Thomas Kavanagh, James A. Levitt et autres
We report the development of a novel massively-parallelised high-speed multifocal FLIM platform with the ability to acquire data 1024 times faster than a conventional TCSPC system. We demonstrate the system performanceFRET imaging of the fluorescent protein biosensor PercivalHR in iPSC derived neurons …
gb
(code pays fourni par la source)
Accès ouvert
2020
article
OpenAlex
Karin Pfisterer, James A. Levitt, Campbell D. Lawson, Richard J. Marsh et autres
Filopodia are peripheral F-actin-rich structures that enable cell sensing of the microenvironment. Fascin is an F-actin-bundling protein that plays a key role in stabilizing filopodia to support efficient adhesion and migration. Fascin is also highly up-regulated in human cancers, where it increases …
gb, us
(code pays fourni par la source)
Accès ouvert
2019
article
OpenAlex
Diane J. Roth, Pavel Ginzburg, Liisa M Hirvonen, James A. Levitt et autres
Abstract The spontaneous emission process is known to be largely affected by the surrounding electromagnetic environment of emitters, which manifests itself via the Purcell enhancement of decay rates. This phenomenon has been extensively investigated in the case of dipolar transitions in quantum …
gb, il, us
(code pays fourni par la source)
Accès ouvert
2019
article
OpenAlex
Penny E. Morton, Camille Perrin, James A. Levitt, Daniel R. Matthews et autres
Signaling by the ubiquitously expressed tumor necrosis factor receptor 1 (TNFR1) after ligand binding plays an essential role in determining whether cells exhibit survival or death. TNFR1 forms distinct signaling complexes that initiate gene expression programs downstream of the transcriptional regulators NFκB …
gb
(code pays fourni par la source)
2019
conference-paper
OpenAlex
Klaus Suhling, Gökhan Yahioglu, Alix Le Marois, Liisa M Hirvonen et autres
Imaging viscosity and its spatiotemporal patterns can provide valuable insight into the underlying physical conditions of biochemical reactions and biological processes in cells and tissues. One way to measure viscosity and diffusion is the use of fluorescence recovery after photobleaching (FRAP). We …
gb
(code pays fourni par la source)