Accès ouvert
2026
article
OpenAlex
Oleksandr Ievglevskyi, Denis Zosen, Jordi Requena Osete, Joel C. Glover et autres
The spinal cord exhibits a complex cytoarchitecture and neuronal circuitry organization that challenges in vitro replication of its functional integrity. Here we employed a previously published protocol to generate spinal cord organoids (SCOs) from human-induced pluripotent stem cells (hiPSCs). We characterized phenotypic …
no, ch, us
(code pays fourni par la source)
Accès ouvert
2026
preprint
OpenAlex
Ibrahim A. Akkouh, Jordi Requena Osete, Attila Szabó, Vidar Steen et autres
ABSTRACT Clozapine (CLZ) is the only available pharmacological option for treatment resistant schizophrenia (TRS), but its use is limited due to adverse drug reactions and potential cytotoxicity. Despite decades of research, the precise mechanisms of action of CLZ in the human brain …
no, de
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Jordi Requena Osete, Attila Szabó, Ibrahim A. Akkouh, Martin Falck et autres
Neuroinflammation and astrocyte dysfunction are associated with schizophrenia. To elucidate the molecular mechanisms involved, we generated induced pluripotent stem cells from 14 schizophrenia patients and 14 healthy controls and differentiated them to human cortical spheroids. Transcriptional profiling revealed overexpression of cilia-related genes …
no, gb
(code pays fourni par la source)
Accès ouvert
2026
preprint
OpenAlex
Ibrahim A. Akkouh, Jordi Requena Osete, Thor Ueland, N. W. Steen et autres
ABSTRACT Schizophrenia (SCZ) is increasingly linked to neuroimmune dysregulation and impaired synaptic plasticity, yet the cellular mechanisms connecting inflammatory signaling to neural dysfunction remain poorly understood. Using human induced pluripotent stem cell (iPSC)-derived cortical spheroids (hCS) and astrocytes from patients with SCZ …
no
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Denis Reis de Assis, Atle Bråthen Pentz, Jordi Requena Osete, Oleksandr Ievglevskyi et autres
Synaptic dysfunction is a candidate mechanism in psychotic disorders, yet the precise underlying substrates remain elusive. We investigated how combining in vivo electroencephalography (EEG) and in vitro human cortical spheroid (hCS)-based methods can further our understanding of psychosis pathophysiology during fetal stages …
no, fi, se
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Jordi Requena Osete, Belén Alvarez Palomo, Michael J. Edel
The most frequent chromosome duplications (whole chromosome and subchromosomal regions) in pluripotent cells are in autosome chromosomes 1, 12, 17 and sex chromosome X.Amplifications in 20q region have been detected in 34% of ESC and iPSC lines examined (Amps et al., 2011;Taapken …
no, es, au
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Ibrahim A. Akkouh, Jordi Requena Osete, Attila Szabó, Ole A. Andreassen et autres
Bipolar disorder (BD) and schizophrenia (SCZ) are uniquely human disorders with a complex pathophysiology that involves adverse neuropathological events in brain development. High disease polygenicity and limited access to live human brain tissue make these disorders exceedingly challenging to study mechanistically. Cellular …
no
(code pays fourni par la source)
2024
article
OpenAlex
Michael J. Edel, Helena Sarret Casellas, Jordi Requena Osete, Núria Nieto‐Nicolau et autres
In adults, the limbal stem cells (LSC) reside in the limbal region of the eye, at the junction of the cornea and the sclera where they renew the outer epithelial layer of the cornea assuring transparency. LSC deficiencies (LSCD) due to disease …
es, au, no
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Attila Szabó, Ibrahim A. Akkouh, Jordi Requena Osete, Dênis Reis de Assis et autres
• SCZ astrocytes express lower levels of NLRP3-ASC corresponding to similar expression patterns in postmortem brain samples. • Higher activity of Caspase-1 upon inflammasome stimulation relative to CTRL in iPSC-astrocytes. • Skewed Th1 and Th17 lymphocyte activation capacity by SCZ astrocytes upon …
no
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Attila Szabó, Kevin S. O’Connell, Ibrahim A. Akkouh, Thor Ueland et autres
• People with ASD display higher plasma levels of IL-18, ICAM-1, MADCAM1, and PSEL relative to CTRL. • In ASD, mRNA levels of ICAM1 is increased in postmortem brain samples relative to controls. • Higher levels and activity of the NLRP3 inflammasome-caspase-1 …
no
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Asbjørn Holmgren, Ibrahim A. Akkouh, Kevin S. O’Connell, Jordi Requena Osete et autres
Bipolar disorder is a severe neuro-psychiatric condition where genome-wide association and sequencing studies have pointed to dysregulated gene expression as likely to be causal. We observed strong correlation in expression between GWAS-associated genes and hypothesised that healthy function depends on balance in …
no
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Iñaki Álvarez, Adrián Tirado-Herranz, Belén Álvarez-Palomo, Jordi Requena Osete et autres
BACKGROUND: Proteins targeted by the ubiquitin proteasome system (UPS) are identified for degradation by the proteasome, which has been implicated in the development of neurodegenerative diseases. Major histocompatibility complex (MHC) molecules present peptides broken down by the proteasome and are involved in …
es, no, au
(code pays fourni par la source)