Devika Bose

AI driven 3D subcellular RPE map discovers cell state transitions in establishment of apical-basal polarity

Davide Ortolan, Pushkar S. Sathe, Andrei Volkov, Dominik Reichert et autres

The retinal pigment epithelium (RPE) is a specialized cell monolayer that forms the barrier between the subretinal and choroidal spaces. During development, RPE cells polarize perpendicular to the monolayer plane such that organelles attain specific intracellular locations. This allows the RPE to …

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A quantitative 3D Subcellular Map of Human Retinal Pigment Epithelium Discovers Non-stochastic Cell State Transition in Establishment of Apical/Basal Polarity

Kapil Bharti, Davide Ortolan, Pushkar S. Sathe, А А Волков et autres

The AKT2/SIRT5/TFEB pathway as a potential therapeutic target in non-neovascular AMD

Sayan Ghosh, Ruchi Sharma, Sridhar Bammidi, Victoria Koontz et autres

Non-neovascular or dry age-related macular degeneration (AMD) is a multi-factorial disease with degeneration of the aging retinal-pigmented epithelium (RPE). Lysosomes play a crucial role in RPE health via phagocytosis and autophagy, which are regulated by transcription factor EB/E3 (TFEB/E3). Here, we find …

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Differentiation of monocytes and polarized M1/M2 macrophages from human induced pluripotent stem cells

Tea Soon Park, Rishabh Hirday, Russell Quinn, Sheela Jacob et autres

Here, we present a protocol to differentiate induced pluripotent stem cell (iPSC) into adherent hematopoietic progenitors that release floating CD14+ CD45+ monocytes into the culture medium. We describe steps for iPSC expansion, embryoid body (EB) formation, suspension culture, plating EBs, and recurring …

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JoVE Video Dataset

Zander Esh, Sharanya Suresh, Davide Ortolan, Mitra Farnoodian et autres

The retinal pigment epithelium (RPE) is a monolayer of hexagonal cells located at the back of the eye. It provides nourishment and support to photoreceptors and choroidal capillaries, performs phagocytosis of photoreceptor outer segments (POS), and secretes cytokines in a polarized manner …

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JoVE Video Dataset

Zander Esh, Sharanya Suresh, Davide Ortolan, Mitra Farnoodian et autres

The retinal pigment epithelium (RPE) is a monolayer of hexagonal cells located at the back of the eye. It provides nourishment and support to photoreceptors and choroidal capillaries, performs phagocytosis of photoreceptor outer segments (POS), and secretes cytokines in a polarized manner …

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The AKT2/SIRT5/TFEB pathway as a potential therapeutic target in atrophic AMD

Sayan Ghosh, Ruchi Sharma, Sridhar Bammidi, Victoria Koontz et autres

Introductory paragraph Age-related macular degeneration (AMD), the leading cause of geriatric blindness, is a multi-factorial disease with retinal-pigmented epithelial (RPE) cell dysfunction as a central pathogenic driver. With RPE degeneration, lysosomal function is a core process that is disrupted. Transcription factors EB/E3 …

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JoVE Video Dataset

Zander Esh, Sharanya Suresh, Davide Ortolan, Mitra Farnoodian et autres

The retinal pigment epithelium (RPE) is a monolayer of hexagonal cells located at the back of the eye. It provides nourishment and support to photoreceptors and choroidal capillaries, performs phagocytosis of photoreceptor outer segments (POS), and secretes cytokines in a polarized manner …

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LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium

Zander Esh, Sharanya Suresh, Davide Ortolan, Mitra Farnoodian et autres

The retinal pigment epithelium (RPE) is a monolayer of hexagonal cells located at the back of the eye. It provides nourishment and support to photoreceptors and choroidal capillaries, performs phagocytosis of photoreceptor outer segments (POS), and secretes cytokines in a polarized manner …

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Considerations for Developing an Autologous Induced Pluripotent Stem Cell (iPSC)-Derived Retinal Pigment Epithelium (RPE) Replacement Therapy

Devika Bose, Davide Ortolan, Mitra Farnoodian, Ruchi Sharma et autres

Cell-replacement therapies are a new class of treatments, which include induced pluripotent stem cell (iPSC)-derived tissues that aim to replace degenerated cells. iPSCs can potentially be used to generate any cell type of the body, making them a powerful tool for treating …

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Retina and RPE lipid profile changes linked with ABCA4 associated Stargardt's maculopathy

Mitra Farnoodian, Devika Bose, Francesca Barone, Luke Mathew Nelson et autres

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Bioprinted 3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration

Min Jae Song, Russ Quinn, Eric Nguyen, Christopher Hampton et autres

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