Holly A. Aliesky

Detection of COVID-19 by quantitative analysis of carbonyl compounds in exhaled breath

Zhenzhen Xie, James Morris, Jianmin Pan, Elizabeth A. Cooke et autres

COVID-19 has caused a worldwide pandemic, creating an urgent need for early detection methods. Breath analysis has shown great potential as a non-invasive and rapid means for COVID-19 detection. The objective of this study is to detect patients infected with SARS-CoV-2 and …

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A feasibility study on exhaled breath analysis using UV spectroscopy to detect COVID-19

Saurin R. Sutaria, James Morris, Zhenzhen Xie, Elizabeth A. Cooke et autres

A 23-subject feasibility study is reported to assess how UV absorbance measurements on exhaled breath samples collected from silicon microreactors can be used to detect COVID-19. The silicon microreactor technology chemoselectively preconcentrates exhaled carbonyl volatile organic compounds and subsequent methanol elution provides …

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Performance of a SARS-CoV-2 RT-PCR Assay with Non-Traditional Specimen Types

Subathra Marimuthu, Holly A. Aliesky, Heather Ness, Daniya Sheikh et autres

During the first two years of the coronavirus disease 2019 (COVID-19) pandemic, nasopharyngeal (NP) specimens were the gold standard for clinical diagnostic testing. As information about the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing the pandemic continued to be shared, it …

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Detection of COVID-19 by Quantitative Analysis of Carbonyl Compounds in Exhaled Breath

Zhenzhen Xie, James Morris, Jianmin Pan, Elizabeth A. Cooke et autres

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The NOD Mouse Beyond Autoimmune Diabetes

A. Aubin, Félix Lombard‐Vadnais, Roxanne Collin, Holly A. Aliesky et autres

Autoimmune diabetes arises spontaneously in Non-Obese Diabetic (NOD) mice, and the pathophysiology of this disease shares many similarities with human type 1 diabetes. Since its generation in 1980, the NOD mouse, derived from the Cataract Shinogi strain, has represented the gold standard …

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Impact of Pooling Samples on Analytic Sensitivity of a Real-Time Reverse Transcriptase PCR Assay for SARS CoV-2

Subathra Marimuthu, Stephen Furmanek, Holly A. Aliesky, Leslie A. Wolf

During the COVID-19 pandemic, laboratories experienced periods of shortages for certain critical materials required to meet the high demand for SARS-CoV-2 testing. The U.S. Food & Drug Administration provided a template for molecular diagnostic testing, including guidance for a specimen pooling process …

Impact of Pooling Samples on Analytic Sensitivity of a Real-Time Reverse Transcriptase PCR Assay for SARS CoV-2

Subathra Marimuthu, Stephen Furmanek, Holly A. Aliesky, Leslie A. Wolf

During the COVID-19 pandemic, laboratories experienced periods of shortages for certain critical materials required to meet the high demand for SARS-CoV-2 testing. The U.S. Food & Drug Administration provided a template for molecular diagnostic testing, including guidance for a specimen pooling process …

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Insulin-like Growth Factor 1 and Prolactin Levels in Chimpanzees (Pan troglodytes) Across the Lifespan

Anat Ben-Shlomo, Sandra M. McLachlan, Jennifer Hwe, Holly A. Aliesky et autres

Abstract As human and chimpanzee genomes show high homology for IGF1 and PRL, we analyzed the sera of 367 healthy chimpanzees obtained during routine physical examinations in a single colony and measured chimpanzee insulin-like growth factor (IGF)-1 and prolactin (PRL) levels across …

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A Mouse Thyrotropin Receptor A-Subunit Transgene Expressed in Thyroiditis-Prone Mice May Provide Insight into Why Graves' Disease Only Occurs in Humans

Sandra M. McLachlan, Holly A. Aliesky, Basil Rapoport

Background: Graves' disease, caused by autoantibodies that activate the thyrotropin (TSH) receptor (TSHR), has only been reported in humans. Thyroiditis-prone NOD. H2 h4 mice develop autoantibodies to thyroglobulin (Tg) and thyroid peroxidase (TPO) but not to the TSHR. Evidence supports the importance …

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Nanoparticles Bearing TSH Receptor Protein and a Tolerogenic Molecule Do Not Induce Immune Tolerance but Exacerbate Thyroid Autoimmunity in hTSHR/NOD.H2h4 Mice

Sandra M. McLachlan, Holly A. Aliesky, Basil Rapoport

Abstract Transgenic NOD.H2h4 mice that express the human (h) TSHR A-subunit in the thyroid gland spontaneously develop pathogenic TSHR autoantibodies resembling those in patients with Graves disease. Nanoparticles coupled to recombinant hTSHR A-subunit protein and a tolerogenic molecule (ligand for the endogenous …

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To reflect human autoimmune thyroiditis, thyroid peroxidase (not thyroglobulin) antibodies should be measured in female (not sex-independent) NOD.H2h4 mice

Sandra M. McLachlan, Holly A. Aliesky, B Rapoport

Summary NOD.H2h4 mice are the most commonly used model for human autoimmune thyroiditis. Because thyroid autoimmunity develops slowly (over months), NOD.H2h4 mice are usually exposed to excess dietary iodide to accelerate and amplify the process. However, unlike the female bias in human …

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Thyroid Hemiagenesis in a Thyroiditis Prone Mouse Strain

Sandra M. McLachlan, Holly A. Aliesky, Priscilla Garcia, Bianca Banuelos et autres

Abstract Background: Thyroid hemiagenesis, a rare congenital condition detected by ultrasound screening of the neck, is usually not manifested clinically in humans. This condition has been reported in mice with hypothyroidism associated with induced deficiency in paired box 8 and NK2 homeobox …

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