Kristin Marie Valand Herstad

DNA extraction protocols for animal fecal material on blood spot cards

Ann‐Katrin Llarena, Thomas H. A. Haverkamp, Wenche Støldal Gulliksen, Kristin Marie Valand Herstad et autres

BACKGROUND: Collecting fecal samples using dry preservatives is an attractive option in large epidemiological studies as they are easy to use, cheap and independent of cold chain logistics. Here, we test four DNA extraction methods with the aim of identifying an efficient …

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DNA Extraction Protocols for Animal Fecal Material on Blood Spot Cards

Ann‐Katrin Llarena, Thomas H. A. Haverkamp, Wenche Støldal Gulliksen, Kristin Marie Valand Herstad et autres

Abstract Background Collecting fecal samples using dry preservatives is an attractive option in large epidemiological studies as they are easy to use, cheap and independent of cold chain logistics. Here, we test four DNA extraction methods with the aim of identifying an …

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Iron‐deficiency anaemia and severe hypocobalaminaemia and hypofolataemia in a dog fed a homemade diet

Kristin Marie Valand Herstad, Benoît Cuq

Abstract A middle‐aged, female, neutered dog was diagnosed with iron‐deficient anaemia on a routine health check following rescue from a puppy mill. Further investigations revealed a severe hypocobalaminaemia and hypofolataemia. The dog was fed a homemade diet consisting of various types of …

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Effects of Canine-Obtained Lactic-Acid Bacteria on the Fecal Microbiota and Inflammatory Markers in Dogs Receiving Non-Steroidal Anti-Inflammatory Treatment

Kristin Marie Valand Herstad, Hilde Vinje, Ellen Skancke, Terese Vatne Naeverdal et autres

Non-steroidal anti-inflammatory drugs (NSAIDs) may cause enteropathy in dogs and probiotics may be one option to prevent this. The objective of this study was to determine whether the administration of canine-obtained lactic acid bacteria (LAB) has an effect on the frequency of …

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Changes in the fecal microbiota in dogs with acute hemorrhagic diarrhea during an outbreak in Norway

Kristin Marie Valand Herstad, Pål Trosvik, Anita Haug Haaland, Thomas H. A. Haverkamp et autres

BACKGROUND: A severe form of acute hemorrhagic diarrhea syndrome (AHDS) occurred in dogs in the Oslo region of Norway during autumn 2019. OBJECTIVES: To characterize the fecal microbiota of dogs with AHDS during the outbreak and compare it to that of healthy …

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Immunohistochemical expression of β-catenin, Ki67, CD3 and CD18 in canine colorectal adenomas and adenocarcinomas

Kristin Marie Valand Herstad, Gjermund Gunnes, Runa Rørtveit, Øyvor Kolbjørnsen et autres

BACKGROUND: Inflammation is believed to influence human colorectal carcinogenesis and may have an impact on prognosis and survival. The mucosal immunophenotype in dogs with colorectal cancer is poorly described. The aim of this study was to investigate whether the density, distribution and …

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Immunohistochemical Expression of β-catenin, Ki67, CD3 and CD18 in Canine Colorectal Adenomas and Adenocarcinomas

Kristin Marie Valand Herstad, Gjermund Gunnes, Runa Rørtveit, Øyvor Kolbjørnsen et autres

Abstract Background Inflammation is believed to influence the human colorectal carcinogenesis and may have impact upon prognosis and survival. High presence of tumour-infiltrating CD3+ T-cells, is associated with a better outcome in humans with colorectal cancer. The mucosal immunophenotype in dogs with …

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Characterization of the fecal and mucosa-associated microbiota in dogs with colorectal epithelial tumors

Kristin Marie Valand Herstad, Aina Elisabeth Fossum Moen, John Christian Gaby, Lars Moe et autres

Colorectal epithelial tumors occur spontaneously in dogs, and the pathogenesis seems to parallel that of humans. The development of human colorectal tumorigenesis has been linked to alterations in the composition of the intestinal microbiota. This study characterized the fecal- and mucosa-associated microbiota …

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Changes in the faecal bile acid profile in dogs fed dry food vs high content of beef: a pilot study

Kristin Marie Valand Herstad, Helene Thorsen Rønning, Anne Marie Bakke, Lars Moe et autres

BACKGROUND: Dogs are fed various diets, which also include components of animal origin. In humans, a high-fat/low-fibre diet is associated with higher faecal levels of bile acids, which can influence intestinal health. It is unknown how an animal-based diet high in fat …

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Intestinal microbiota, red meat, and colorectal tumours in dogs

Kristin Marie Valand Herstad

Tarmens mikrobiota består av et rikt nettverk av mikrober som har betydning for vertens fysiologi, og således helse og velvære. Mikrobiotaen påvirkes av diettens sammensetning av makronæringsstoffer (karbohydrater, proteiner, fettstoffer og ikke-fordøyelige karbohydrater). Hos mennesker er høyt inntak av rødt kjøtt, f.eks. …

MOESM1 of Changes in the faecal bile acid profile in dogs fed dry food vs high content of beef: a pilot study

Kristin Marie Valand Herstad, Helene RĂ ̧Nning, Anne Marie Bakke, Lars Moe et autres

Additional file 1. Ingredients and nutrient composition of the rations during the seven-week dietary intervention study (a), and main ingredients in CD (b).

A diet change from dry food to beef induces reversible changes on the faecal microbiota in healthy, adult client-owned dogs

Kristin Marie Valand Herstad, Karina Gajardo, Anne Marie Bakke, Lars Moe et autres

BACKGROUND: Diet has a major influence on the composition of the gut microbiota, whose importance for gut health and overall well-being is increasingly recognized. Knowledge is limited regarding health implications, including effects on the faecal microbiota, of feeding a diet with high …

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