Evan F. Cromwell

Protocols and research articles in 3D biology: technologies and methodologies reshaping 3D cell culture

Glauco R. Souza, Evan F. Cromwell

Abstract LB171: Drug response profiling of patient-derived tumoroids and immortalized cancer cell lines using a novel automation workflow and AI-assisted image analysis

Anthony S. Eritano, Dylan Bryda, Andreea Merloiu, Alissa Schutte et autres

Abstract Three-dimensional in vitro cell models have gained popularity as they better recapitulated key aspects of human tissues and tumor microenvironments. Forming structures with patient-derived (PD) cancer cells show great promise for translational work. These 3D models are being used for drug …

us (code pays fourni par la source)

Abstract 4559: Evaluation of the chemotherapy potential to improve the anti-cancer efficacy of PD-L1 inhibition using novel 3D microfluidic co-culture platform

Ekaterina Moroz Nikolov, Anthony Thai, Lila Cooper, Mahomi Suzuki et autres

Abstract Immunotherapy is now recognized as a powerful therapeutic approach to treat and cure cancer. However, the objective response rates for most solid tumors are low (below 30%). Accumulating evidence has shown that chemotherapy can increase the efficacy of immune checkpoint blockade …

jp, us (code pays fourni par la source)

Evaluating Drug Response in 3D Triple Negative Breast Cancer Tumoroids with High Content Imaging and Analysis

Oksana Sirenko, Courtney K. Brock, Angeline Pei Chiew Lim, Prathyushakrishna Macha et autres

Abstract There is a critical need to develop methods for efficient testing of drug efficacy in patient-derived tumor samples to discover new therapeutics. Two-dimensional (2D) cell culture remains the primary method of drug screening, despite being considered less physiologically relevant than three-dimensional …

us (code pays fourni par la source)

Abstract 180: Automation and high content imaging of 3D triple negative breast cancer patient-derived tumoroids assay for compound screening

Oksana Sirenko, Angeline Pei Chiew Lim, Courtney K. Brock, Katya Nikolov et autres

Abstract Introduction: Currently there are no clinically approved small molecule targeted therapies for triple negative breast cancer, underlining the critical need to discover new therapeutic targets. Patient-derived cell-based 3D cancer models are highly valuable tools for cancer research and drug development. Primary …

us (code pays fourni par la source)

Abstract 185: Monitoring metabolite dynamics in patient-derived tumoroids using automated microfluidic Pu·MA System

Ekaterina Moroz Nikolov, Oksana Sirenko, Matthew A. Hammer, Courtney K. Brock et autres

Abstract Introduction: Cancer cells are characterized by metabolic modifications to meet high bioenergetic and anabolic demand during tumorigenesis. For most tumors aerobic glycolysis is a major driver of cancer progression, resistance to therapy, poor patient outcome. Lactate, a product of glycolysis, plays …

us (code pays fourni par la source)

Multifunctional profiling of triple-negative breast cancer patient-derived tumoroids for disease modeling

Evan F. Cromwell, Oksana Sirenko, Ekaterina Nikolov, Matthew A. Hammer et autres

3D cell models derived from patient tumors are highly translational tools that can recapitulate the complex genetic and molecular compositions of solid cancers and accelerate identification of drug targets and drug testing. However, the complexity of performing assays with such models remains …

us (code pays fourni par la source)

In-depth characterization of a new patient-derived xenograft model for metaplastic breast carcinoma to identify viable biologic targets and patterns of matrix evolution within rare tumor types

Margarite D. Matossian, Tiffany C. Chang, Maryl K. Wright, Hope E. Burks et autres

Metaplastic breast carcinoma (MBC) is a rare breast cancer subtype with rapid growth, high rates of metastasis, recurrence and drug resistance, and diverse molecular and histological heterogeneity. Patient-derived xenografts (PDXs) provide a translational tool and physiologically relevant system to evaluate tumor biology …

us (code pays fourni par la source)

Disease Modeling with 3D Cell-Based Assays Using a Novel Flowchip System and High-Content Imaging

Evan F. Cromwell, Michelle M. Leung, Matthew A. Hammer, Anthony Thai et autres

There is an increasing interest in using three-dimensional (3D) cell structures for modeling tumors, organs, and tissue to accelerate translational research. We describe here a novel automated organoid assay system (the Pu·MA System) combined with microfluidic-based flowchips that can facilitate 3D cell-based …

us (code pays fourni par la source)

Assessment of beating parameters in human induced pluripotent stem cells enables quantitative in vitro screening for cardiotoxicity

Jessica Wignall, Oksana Sirenko, Evan F. Cromwell, Carole Crittenden et autres

Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes show promise for screening during early drug development. Here, we tested a hypothesis that in vitro assessment of multiple cardiomyocyte physiological parameters enables predictive and mechanistically-interpretable evaluation of cardiotoxicity in a high-throughput format. Human iPSC-derived …

High-Content Assays for Hepatotoxicity Using Induced Pluripotent Stem Cell–Derived Cells

Evan F. Cromwell, Jayne Hesley, Ivan Rusyn, Oksana Sirenko

Development of predictive in vitro assays for early toxicity evaluation is extremely important for improving the drug development process and reducing drug attrition rates during clinical development. High-content imaging-based in vitro toxicity assays are emerging as efficient tools for safety and efficacy …

Multi-parametric Cell-Based Inflammation Assays for Cytokines and Cell Surface Antigens

Evan F. Cromwell, Byungrae Cho, Ori Hoxha, Neil Bristol et autres

Abstract Macrophages and vascular cells upregulate expression of cytokines and adhesion molecules in response to inflammation stimuli. Monitoring these molecules can provide a physiological read-out for inflammation. We present results from a multiparametric assays that used a combination of low-volume ELISA for …