Karin Anna Hummel

Pharmacokinetics of ropivacaine after intraperitoneal administration to dogs undergoing ovariectomy or ovariohysterectomy: preliminary results

Natalí Verdier, Karin Anna Hummel, Svenja Claaßen, Pier Arturo Donati et autres

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Pioneer bark beetle attacks induce multifaceted localized defense responses in Norway spruce

Marcelo de Jesus Ramires, Sigrid Netherer, Martin Schebeck, Karin Anna Hummel et autres

Abstract Conifer forests worldwide are increasingly threatened by environmental stressors, especially bark beetle infestations. In a novel experimental field setup with controlled attacks on clonal trees, we investigated the molecular defense responses of 35-year-old Norway spruce trees to colonization attempts by the …

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The inner dynamics of positive human-animal interactions: investigating the roles of oxytocin, opioids, dopamine, serotonin and the proteome

Océane Schmitt, Suzanne Truong, Winfried Otten, Marlies Dolezal et autres

Abstract Research on the neuroendocrine basis of positive interactions has predominantly focused on oxytocin (OT), although dopamine (DA) and opioids also play crucial roles. Furthermore, these neurotransmitters are known to interact with each other but have seldom been studied concurrently. In this …

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Protocol for Proteome Extraction from Norway Spruce Bark Samples v1

Marcelo de Jesus Ramires, Sarah Schlosser, Karin Anna Hummel, Ebrahim Razzazi‐Fazeli et autres

Proteomic analysis of Norway spruce bark presents unique challenges due to the high content of non-protein contaminants like phenolic compounds and polysaccharides. The use of more conventional methods such as TCA-acetone precipitation often falls short in such contexts. Here we present an …

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The proteomic landscape of Toxoplasma gondii extracellular vesicles across diverse host cell types

Teresa Cruz Bustos, Anna Sophia Feix, Karin Anna Hummel, Sarah Schlosser et autres

Introduction Extracellular vesicles (EVs) are emerging as powerful tools used by pathogens to manipulate host cells, delivering molecular cargo that rewires cellular processes and the immune response. Toxoplasma gondii, a globally distributed parasite capable of infecting nearly all nucleated animal cells, uses …

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Locus-specific chromatin proteomics using dCas-guided proximity labelling in Aspergillus nidulans

Thomas Svoboda, Dominik Niederdöckl-Loibl, Andreas Schüller, Karin Anna Hummel et autres

Proximity labelling that uses promiscuous biotin ligases (BirA) fused to a bait protein is a powerful tool to identify protein interaction partners in vivo under different metabolic or developmental conditions. BirA can also be used to determine protein composition and interaction partners …

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Host-specific targets of Histomonas meleagridis antigens revealed by immunoprecipitation

Marcelo de Jesus Ramires, Karin Anna Hummel, Tamás Hatfaludi, Michael L Hess et autres

Histomonas meleagridis, a protozoan parasite responsible for histomonosis (syn. Blackhead disease, histomoniasis), presents an increasing challenge for poultry health, particularly with the ban of licensed prophylactic and treatment options. Recent studies have explored H. meleagridis proteome, exoproteome, and surfaceome, linking molecular data …

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Comprehensive phenotyping combined with multi-omics of Salmonella Infantis and its H2S negative variant - Resolving adaption mechanisms to environmental changes

Victoria Drauch, Nicola Palmieri, Joachim Spergser, Karin Anna Hummel et autres

The zoonotic pathogen S. Infantis is of emerging importance, making detection in poultry critical. Phenotypic changes, which are significant for standardized control programs via EN/ISO 6579-1:2017, could lead to pathogens remaining undetected, increasing the risk of food-borne outbreaks. This study investigates an …

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Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations

Ezequias Castillo‐Lopez, Patrick Biber, Arife Sener-Aydemir, Karin Anna Hummel et autres

BACKGROUND: The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G (IgG) content. Immediately after calving, colostrum samples were collected from 18 cows to measure IgG concentration. Based on colostrum IgG content, samples were classified …

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Locus-specific chromatin proteomics using dCas-guided proximity labelling in Aspergillus nidulans

Thomas Svoboda, Dominik Niederdöckl-Loibl, Andreas Schüller, Karin Anna Hummel et autres

Abstract Proximity labelling that uses promiscuous biotin ligases (BirA) fused to a bait protein is a powerful tool to identify protein interaction partners in vivo under different metabolic or developmental conditions. BirA can also be used to determine protein composition and interaction …

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Comparative proteomic analysis of metronidazole-sensitive and resistant Trichomonas vaginalis suggests a novel mode of metronidazole action and resistance

Anna-Lena Mayr, Ana Paunkov, Karin Anna Hummel, Ebrahim Razzazi‐Fazeli et autres

The microaerophilic parasite Trichomonas vaginalis occurs worldwide and causes inflammation of the urogenital tract, especially in women. With 156 million infections annually, trichomoniasis is the most prevalent non-viral sexually transmitted disease. Trichomoniasis is treated with 5-nitroimidazoles, especially metronidazole, which are prodrugs that …

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The myxozoans Myxobolus cerebralis and Tetracapsuloides bryosalmonae modulate rainbow trout immune responses: quantitative shotgun proteomics at the portals of entry after single and co-infections

Mona Nima Saleh, Karin Anna Hummel, Sarah Schlosser, Ebrahim Razzazi‐Fazeli et autres

Introduction Little is known about the proteomic changes at the portals of entry in rainbow trout after infection with the myxozoan parasites, Myxobolus cerebralis , and Tetracapsuloides bryosalmonae . Whirling disease (WD) is a severe disease of salmonids, caused by the myxosporean …

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