Jordan Anderson-Daniels

The coronavirus nsp14 exoribonuclease interface with the cofactor nsp10 is essential for efficient virus replication and enzymatic activity

Samantha L. Grimes, Brook E. Heaton, Mackenzie L. Anderson, Katie Burke et autres

ABSTRACT Coronaviruses (CoVs) encode non-structural proteins (nsp’s) 1–16, which assemble to form replication-transcription complexes that function in viral RNA synthesis. All CoVs encode a proofreading 3′−5′ exoribonuclease in non-structural protein 14 (nsp14-ExoN) that mediates proofreading and high-fidelity replication and is critical for …

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The coronavirus nsp14 exoribonuclease interface with the cofactor nsp10 is essential for efficient virus replication and enzymatic activity

Samantha L. Grimes, Brook E. Heaton, Mackenzie L. Anderson, Katie Burke et autres

ABSTRACT Coronaviruses (CoVs) encode nonstructural proteins (nsps) 1-16, which assemble to form replication-transcription complexes that function in viral RNA synthesis. All CoVs encode a proofreading 3’-5’ exoribonuclease (ExoN) in nsp14 (nsp14-ExoN) that mediates proofreading and high-fidelity replication and is critical for other …

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A mutation in the coronavirus nsp13-helicase impairs enzymatic activity and confers partial remdesivir resistance

Samantha L. Grimes, Young Joo Choi, Anoosha Banerjee, Gabriel I. Small et autres

Coronaviruses (CoVs) encode nonstructural proteins 1-16 (nsps 1-16) which form replicase complexes that mediate viral RNA synthesis. Remdesivir (RDV) is an adenosine nucleoside analog antiviral that inhibits CoV RNA synthesis. RDV resistance mutations have been reported only in the nonstructural protein 12 …

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Proteolytic Processing of the Coronavirus Replicase Nonstructural Protein 14 Exonuclease Is Not Required for Virus Replication but Alters RNA Synthesis and Viral Fitness

Jordan Anderson-Daniels, Jennifer Gribble, Mark R. Denison

Coronavirus replication requires proteolytic maturation of the nonstructural replicase proteins to form the replication-transcription complex. Coronavirus replication-transcription complex models assume mature subunits; however, mechanisms of coronavirus maturation and replicase complex formation have yet to be defined. Here, we show that for the …

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The coronavirus proofreading exoribonuclease mediates extensive viral recombination

Jennifer Gribble, Laura J. Stevens, Maria L. Agostini, Jordan Anderson-Daniels et autres

Recombination is proposed to be critical for coronavirus (CoV) diversity and emergence of SARS-CoV-2 and other zoonotic CoVs. While RNA recombination is required during normal CoV replication, the mechanisms and determinants of CoV recombination are not known. CoVs encode an RNA proofreading …

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Intrinsic curvature of the HIV-1 CA hexamer underlies capsid topology and interaction with cyclophilin A

Tao Ni, Samuel F. Gérard, Gongpu Zhao, Kyle C. Dent et autres

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The coronavirus proofreading exoribonuclease mediates extensive viral recombination

Jennifer Gribble, Andrea J. P. Pruijssers, Maria L. Agostini, Jordan Anderson-Daniels et autres

SUMMARY Coronaviruses (CoVs) emerge as zoonoses and cause severe disease in humans, demonstrated by the SARS-CoV-2 (COVID-19) pandemic. RNA recombination is required during normal CoV replication for subgenomic mRNA (sgmRNA) synthesis and generates defective viral genomes (DVGs) of unknown function. However, the …

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Dominant Negative MA-CA Fusion Protein Is Incorporated into HIV-1 Cores and Inhibits Nuclear Entry of Viral Preintegration Complexes

Jordan Anderson-Daniels, Parmit Kumar Singh, Gregory A. Sowd, Wen Li et autres

To become infectious, newly formed HIV-1 particles undergo a process of maturation in which the viral polyproteins are cleaved into smaller components. A previous study demonstrated that inclusion of even small quantities of an uncleavable mutant Gag polyprotein results in a strong …

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