Timothy C. Rodwell

Cepheid 9-gene host mRNA expression across the spectrum of Mycobacterium tuberculosis infection and disease: insights across the lifespan

Marva Seifert, Michael Gracia, Donald G. Catanzaro, Faiqa Rashid et autres

BACKGROUND: Novel diagnostic solutions using host-response-based gene signatures to differentiate between individuals infected with Mycobacterium tuberculosis presenting with active tuberculosis disease (ATB), individuals infected with the bacterium in the absence of active tuberculosis disease (TBI), and individuals with no evidence of ATB …

us, pk, in (code pays fourni par la source)

Implications of edge cases for antimicrobial susceptibility predictions for rifampin, delamanid, and pretomanid with the WHO Mycobacterium tuberculosis mutation catalog

Noud Hermans, Jody Phelan, Rita Sorrentino, Fenja Boysen et autres

ABSTRACT The WHO mutation catalog is the reference for genotypic antimicrobial susceptibility predictions (ASPs) for Mycobacterium tuberculosis . To increase sensitivity, WHO endorsed additional grading rules to classify mutations with insufficient experimental evidence. For rifampin, this includes any non-silent rpoB mutation in …

nl, in, gb, it, Maroc, au, us, de, np, jp, kg, ru (code pays fourni par la source)

Microbial biomarkers of tuberculosis infection and disease in blood: systematic review and meta-analysis

Shruthi Chandran, Edward Cruz Cervera, David A. Jolliffe, Divya Tiwari et autres

ABSTRACT Background Numerous studies reporting utility of microbial blood biomarkers for diagnosis and treatment monitoring of M. tuberculosis (Mtb) infection and tuberculosis disease have been conducted, but up-to-date systematic reviews and meta-analyses of these data are lacking. We aimed to evaluate diagnostic …

gb, Afrique du Sud, us (code pays fourni par la source)

Bedaquiline resistance in patients with Xpert MTB/RIF Ultra-tested rifampicin-resistant tuberculosis in the Western Cape, South Africa: a prospective study

Janré Steyn, Jennifer Williams, Fahd Naufal, Jason Limberis et autres

BACKGROUND: Bedaquiline-containing regimens have been widely used to treat patients with drug-resistant tuberculosis in South Africa since 2019. We aimed to estimate the prevalence of bedaquiline resistance among patients in the Western Cape with rifampicin-resistant tuberculosis tested by Xpert MTB/RIF Ultra (Xpert). …

Afrique du Sud, us (code pays fourni par la source)

Benchmarking generative AI tools for interpretation of the WHO TB mutation catalogue

Miguel Moreno-Molina, Anita Suresh, Rebecca E. Colman, Timothy C. Rodwell

is a crucial knowledgebase and tool for clinical interpretation of mutations associated with drug-resistant TB. However, the document's complexity and size pose challenges for many users. This study evaluated the potential of generative artificial intelligence (AI) models to facilitate natural language user …

ch, us (code pays fourni par la source)

Microbial Biomarkers of Tuberculosis Infection and Disease in Blood: Systematic Review and Meta-Analysis

Shruthi Chandran, Edward Cruz Cervera, David A. Jolliffe, Divya Tiwari et autres

gb, us (code pays fourni par la source)

Clinical evaluation of a commercial culture-free targeted next-generation sequencing test for diagnosis of drug-resistant tuberculosis

Paolo Miotto, Rebecca E. Colman, Andrea Maurizio Cabibbe, Paola M. V. Rancoita et autres

genomes and faster reporting compared to phenotypic drug susceptibility testing (DST). We conducted a prospective, cross-sectional, multicenter evaluation of the DeepChek 13-Plex KB Drug Susceptibility Testing (ABL Diagnostics S.A., France) tNGS assay for direct detection of TB drug resistance from clinical sputum …

it, ch, us, in, ge, Afrique du Sud (code pays fourni par la source)

Minimal immune cell subset differences in a cohort of close contacts of tuberculosis index cases

Sudhasini Panda, Catherine Cheng, Naomi Hillery, Donald G. Catanzaro et autres

Understanding the perturbations in immune response across the spectrum of TB infection is still unclear. Here, we followed close contacts of pulmonary TB patients with serial QFT testing at 0, 3, 6, and 12 months, and stratified them into six subgroups: QFT-increasing …

us, md, dk (code pays fourni par la source)

Comparing end-user diagnostic outputs from a commercial tNGS pipeline for Mycobacterium tuberculosis drug resistance detection

Marva Seifert, Rebecca E. Colman, Sacha Laurent, Andres De la Rossa et autres

SUMMARY BACKGROUND Targeted next-generation sequencing has emerged as a rapid solution for diagnosing drug-resistant TB (DR-TB) directly from clinical specimens. Updating the bioinformatics software component can lead to rapid improvements in diagnostic performance. We compared the diagnostic performance of an updated bioinformatic …

ch, us, in, ge, Afrique du Sud (code pays fourni par la source)

Cost-effectiveness of Targeted Next-Generation Sequencing for Tuberculosis Drug-resistance Testing as an Alternative to the Standard of Care in South Africa

Alexandra de Nooy, Shaheed Vally Omar, Tom Ockhuisen, Alice Zwerling et autres

BACKGROUND: South Africa faces emerging resistance to tuberculosis drugs like bedaquiline. Phenotypic drug-susceptibility testing (DST), the current reference standard for bedaquiline DST, has long turnaround times. Targeted next-generation sequencing (tNGS) offers a comprehensive alternative, potentially delivering faster results. However, these advantages must …

nl, Afrique du Sud, ca, ch, us (code pays fourni par la source)

Comparative assessment of line probe assays and targeted next-generation sequencing in drug-resistant tuberculosis diagnosis

Giovanna Carpi, Marva Seifert, Andres De la Rossa, Swapna Uplekar et autres

BACKGROUND: Rapid and accurate detection of drug-resistant tuberculosis (DR-TB) is crucial for ensuring effective treatment, halting transmission and preventing the amplification of resistance. Comparative evaluations of molecular diagnostic assays in high-burden settings are essential for informing clinical decision-making for DR-TB treatment. METHODS: …

ch, us, in, ge, Afrique du Sud (code pays fourni par la source)

Minimal immune cell subset differences in a cohort of close contacts of tuberculosis index cases

Sudhasini Panda, Catherine Cheng, Naomi Hillery, Donald G. Catanzaro et autres

ABSTRACT Understanding the perturbations in immune response across the spectrum of TB infection is still unclear. In this study, we followed a cohort of close contacts of pulmonary TB patients with serial QFT testing at 0, 3, 6, and 12 months, and …

us, md, dk (code pays fourni par la source)