Panagiota Papazafiri

Neurosteroids Alter p-ERK Levels and Tau Distribution, Restraining the Effects of High Extracellular Calcium

Vasiliki Konsta, Maria Paschou, Nikoleta Koti, Maria Evangelia Vlachou et autres

Neurosteroids are undeniably regarded as neuroprotective mediators, regulating brain function by rapid non-genomic actions involving interference with microtubules. Conversely, hyperphosphorylated Tau is considered responsible for the onset of a plethora of neurodegenerative diseases, as it dissociates from microtubules, leading to their destabilization, …

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The Epigenetic Controller Lysine-Specific Demethylase 1 (LSD1) Regulates the Outcome of Hepatitis C Viral Infection

Georgia Papadopoulou, Stavroula Petroulia, Eirini Karamichali, Alexios Dimitriadis et autres

Hepatitis C virus (HCV) alters gene expression epigenetically to rearrange the cellular microenvironment in a beneficial way for its life cycle. The host epigenetic changes induced by HCV lead to metabolic dysfunction and malignant transformation. Lysine-specific demethylase 1 (LSD1) is an epigenetic …

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Knockdown of Amyloid Precursor Protein Increases Ion Channel Expression and Alters Ca2+ Signaling Pathways

Maria Paschou, Danai Liaropoulou, Vasileia Kalaitzaki, Spiros Efthimiopoulos et autres

Although the physiological role of the full-length Amyloid Precursor Protein (APP) and its proteolytic fragments remains unclear, they are definitively crucial for normal synaptic function. Herein, we report that the downregulation of APP in SH-SY5Y cells, using short hairpin RNA (shRNA), alters …

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Neuronal microRNAs safeguard ER Ca2+ homeostasis and attenuate the unfolded protein response upon stress

Maria Paschou, Panagiota Papazafiri, Chrysanthi Charalampous, Michael Zachariadis et autres

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Enhanced Ca2+ Entry Sustains the Activation of Akt in Glucose Deprived SH-SY5Y Cells

Maria Kourti, Danai Liaropoulou, Maria Paschou, Ioanna Giagklisi et autres

The two crucial cellular insults that take place during cerebral ischemia are the loss of oxygen and loss of glucose, which can both activate a cascade of events leading to neuronal death. In addition, the toxic overactivation of neuronal excitatory receptors, leading …

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A glucose-based molecular rotor inhibitor of glycogen phosphorylase as a probe of cellular enzymatic function

Michail P. Minadakis, Konstantinos F. Mavreas, Dionysios D. Neofytos, Maria Paschou et autres

, it behaved as a light switch, generating a strong fluorescence signal, allowing utilization of RotA as a probe that locates glycogen phosphorylase (GP). RotA, mono-, di- and per-acetylated derivatives, as well as nanoparticles with RotA encapsulated in polyethylene glycol-poly-L-histidine, were used …

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Glucose-Based Molecular Rotors as Fluorescent Inhibitors and Probes of Glycogen Phosphorylase

Konstantinos F. Mavreas, Michael Mamais, Panagiota Papazafiri, Thanasis Gimisis

In this study, (E)-2-cyano-3-(6-(dimethylamino)naphthalen-2-yl)-N-(β-d-glucopyranosyl)acrylamide, a β-d-glucopyranosyl analogue of the widely used molecular rotor julolidine, was synthesized and studied photochemically. The new compound is a fluorescent inhibitor of rabbit muscle glycogen phosphorylase with properties of a molecular rotor. Fluorescence measurements in solutions of …

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Amyloid-β Protein Precursor Regulates Depolarization-Induced Calcium-Mediated Synaptic Signaling in Brain Slices

Maria Chatzistavraki, Panagiota Papazafiri, Spiros Efthimiopoulos

BACKGROUND: Coordinated calcium influx upon neuronal depolarization activates pathways that phosphorylate CaMKII, ERKs, and the transcription factor CREB and, therefore, expression of pro-survival and neuroprotective genes. Recent evidence indicates that amyloid-β protein precursor (AβPP) is trafficked to synapses and promotes their formation. …

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Neuronal microRNAs modulate TREK two-pore domain K+ channel expression and current density

Maria Paschou, Larisa Maier, Panagiota Papazafiri, Tudor Şelescu et autres

The TREK family of leak potassium channels has been found to play critical roles in nociception, sensitivity to general anaesthetics, neuroprotection, and memory. The three members of the family, TREK1, TREK2 and TRAAK establish the resting potential and modify the duration, frequency …

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Neuronal microRNAs modulate TREK two-pore domain K+ channel expression and current density

Maria Paschou, Larisa Maier, Panagiota Papazafiri, Tudor Şelescu et autres

The TREK family of leak potassium channels has been found to play critical roles in nociception, sensitivity to general anaesthetics, neuroprotection, and memory. The three members of the family, TREK1, TREK2 and TRAAK establish the resting potential and modify the duration, frequency …

Neuronal microRNAs modulate TREK two-pore domain K+ channel expression and current density

Maria Paschou, Larisa Maier, Panagiota Papazafiri, Tudor Şelescu et autres

The TREK family of leak potassium channels has been found to play critical roles in nociception, sensitivity to general anaesthetics, neuroprotection, and memory. The three members of the family, TREK1, TREK2 and TRAAK establish the resting potential and modify the duration, frequency …

Amyloid precursor protein regulates depolarization-induced calcium-mediated signaling in brain slices

Spiros Efthimiopoulos, Maria Chatzistavraki, Panagiota Papazafiri

L-type Voltage Gated Calcium Channels (L-VGCCs) mediate calcium influx upon neuronal depolarization, which activates pathways that phosphorylate CaMKII, ERKs and the transcription factor CREB and as a consequence the expression of pro-survival and neuroprotective genes such as Bcl-2 and BDNF.Recent evidence indicates …

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