Tsuyoshi Hayashi

Treatment With Ruxolitinib and TAK ‐779 Enhances GII .17 Human Norovirus Replication and Enables Serial Passaging in Human Intestinal Enteroids

Eri Hiraishi, Tsuyoshi Hayashi, Yoshiki Fujii, Hirokazu Kimura et autres

Human norovirus (HuNoV) is a major cause of acute viral gastroenteritis. Although it can be replicated in vitro using human intestinal enteroids (HIEs), generating high-titer viral laboratory stocks remains challenging due to low infection and passage efficiencies. Interferon response and chemokine signaling …

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Ephedrae Herba as a potential source of SARS-CoV-2 RNA-dependent RNA polymerase inhibitory activity

Yuka Kiba, Tsuyoshi Hayashi, Hirokazu Ando, Hitoshi Kamauchi et autres

Introduction Maoto (麻黄湯) is a traditional Japanese Kampo formula composed of four crude drugs—Ephedrae Herba, Armeniacae Semen, Cinnamomi Cortex, and Glycyrrhizae Radix—and has been used to treat febrile viral illnesses. Among these, Ephedrae Herba has been associated with antiviral activity in previous …

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Establishment of a green fluorescent protein (GFP)-based reporter for picornaviral 3C proteases

Junki Hirano, Tsuyoshi Hayashi, Yuichi Someya, Kazuma Okada et autres

ABSTRACT Picornaviruses represent a diverse group of plus-stranded RNA viruses, many of which have been linked to severe diseases in both humans and animals. The viral 3C protease is essential for the maturation of viral proteins and the propagation of picornaviruses and, …

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Correction: Inhibitory effect of [6]-shogaol against 3CLpro activity and SARS-CoV-2 infection

Takashi Tanikawa, Tsuyoshi Hayashi, Yuka Kiba, Hitoshi Kamauchi et autres

was noted that due to a typesetting error the figure legends were paired incorrectly.The figure legends for Figs. 2 and 4 were wrongly given as captions for Fig. 4 and 2 respectively.The correct figures and captions have been included in this correction, …

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Inhibitory effect of [6]-shogaol against 3CLpro activity and SARS-CoV-2 infection

Takashi Tanikawa, Tsuyoshi Hayashi, Yuka Kiba, Hitoshi Kamauchi et autres

BACKGROUND: Zingiber officinale Roscoe (ginger) has been traditionally used not only as a culinary ingredient, but also for its medicinal properties. In Japan, ginger-derived crude drugs are categorized into dried ginger (Zingiberis Rhizoma [ZR]) or steamed ginger (Zingiberis Rhizoma processum [ZR-P]). Heating …

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PT2.14.03 Molecular Correlates of Response to Atezolizumab Plus Carboplatin and Paclitaxel in Patients With Thymic Carcinoma

Yoko Shirai, Takehiro Shukuya, Takayuki Asao, Y. Goto et autres

jp, us (code pays fourni par la source)

Establishment of a green fluorescent protein (GFP)-based reporter for picornaviral 3C proteases

Junki Hirano, Tsuyoshi Hayashi, Yuichi Someya, Kazuma Okada et autres

Abstract Picornaviruses represent a diverse group of plus-stranded RNA viruses, many of which have been linked to severe diseases in both humans and animals. The viral 3C protease is essential for the maturation of viral proteins and the propagation of picornaviruses and, …

jp (code pays fourni par la source)

Identification of FDA-Approved Drugs That Inhibit SARS-CoV-2 and Human Norovirus Replication

Tsuyoshi Hayashi, Junki Hirano, Kosuke Murakami, Yoshiki Fujii et autres

In this study, to identify novel compounds that inhibit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication by targeting its protease, we screened an U.S. Food and Drug Administration (FDA)-approved drug library to determine their effects on SARS-CoV-2 3CL protease (3CLpro) activity …

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Evaluation of anti-noroviral compounds on human norovirus replication using 2D monolayers of human intestinal organoids

Tsuyoshi Hayashi, Yoshiki Fujii, Junki Hirano, Sakura Kobayashi et autres

Abstract We evaluated the anti-noroviral effects of compounds including molnupiravir, on human norovirus (HuNoV) replication using 2D monolayers of human intestinal organoids (HIOs), which have been widely used in HuNoV infection studies. We found that these compounds exhibited lower antiviral activity in …

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LBA8 Phase II trial of atezolizumab plus carboplatin and paclitaxel in patients with metastatic or recurrent thymic carcinoma: Marble study

Tomoyasu Mimori, Takehito Shukuya, Y. Goto, Takayuki Asao et autres

jp, cn (code pays fourni par la source)

Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis through interaction with GBF1

Junki Hirano, Tsuyoshi Hayashi, KOUICHI KITAMURA, Yorihiro Nishimura et autres

Enteroviruses are single-stranded, positive-sense RNA viruses causing endoplasmic reticulum (ER) stress to induce or modulate downstream signaling pathways known as the unfolded protein responses (UPR). However, viral and host factors involved in the UPR related to viral pathogenesis remain unclear. In the …

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Recent advancements in image‐enhanced endoscopy in the pancreatobiliary field

Haruka Toyonaga, Tsuyoshi Hayashi, Kazuki Hama, Ryõ Andõ et autres

Image-enhanced endoscopy (IEE) has advanced gastrointestinal disease diagnosis and treatment. Traditional white-light imaging has limitations in detecting all gastrointestinal diseases, prompting the development of IEE. In this review, we explore the utility of IEE, including texture and color enhancement imaging and red …

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