2026
preprint
OpenAlex
Tania Miguel Trabajo, Isaline Guex, Helena Todorov, Xavier Richard et autres
ABSTRACT Microbial communities spontaneously colonize pristine environments, yet how species growth kinetics and individual cell variation shape community assembly remains poorly understood. Here, we use time-lapse microscopy imaging to track the division of individual founder cells in communities composed of up to …
ch
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Maxime Batsch, Isaline Guex, Helena Todorov, Clara Margot Heiman et autres
Bacteria in nature often thrive in fragmented environments, like soil pores, plant roots or plant leaves, leading to smaller isolated habitats, shared with fewer species. This spatial fragmentation can significantly influence bacterial interactions, affecting overall community diversity. To investigate this, we contrast …
ch
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Tania Miguel Trabajo, Isaline Guex, Manupriyam Dubey, Elvire Sarton-Lohéac et autres
Abstract Interactions between species are thought to be crucial for modulating their growth and behaviour within communities, and determinant for the emergence of community functions. Several different interaction concepts exist, but there is no consensus on how interactions should be quantified and …
ch
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Helena Todorov, Bouke Bentvelsen, Stefano Ugolini, Alan R. Pacheco et autres
ABSTRACT Real-time imaging of bacterial cell division, population growth and behaviour is essential for our understanding of microbial-catalyzed processes at the microscale. However, despite the relative ease by which high resolution imaging data can be acquired, the extraction of relevant cell features …
ch
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Maxime Batsch, Isaline Guex, Helena Todorov, Clara Margot Heiman et autres
Abstract Bacteria in nature often proliferate in highly patchy environments, such as soil pores, particles, plant roots or leaves. The resulting spatial fragmentation leads to cells being constrained to smaller habitats, shared with potentially fewer other species. The effects of microhabitats on …
ch
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Tania Miguel Trabajo, Isaline Guex, Manupriyam Dubey, Elvire Sarton-Lohéac et autres
Abstract Bacterial species interactions significantly shape growth and behavior in communities, determining the emergence of community functions. Typically, these interactions are studied through bulk population measurements, overlooking the role of cell-to-cell variability and spatial context. This study uses real-time surface growth measurements …
ch
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Helena Todorov, Tania Miguel Trabajo, Jan Roelof van der Meer
Automated image analysis of growing prokaryotic cell populations becomes indispensable with larger data sets, such as derived by time-lapse microscopy. The tracking of the same individual cells and their daughter lineages is cumbersome and prone to errors in image alignment or poor …
ch
(code pays fourni par la source)
Accès ouvert
2023
article
OpenAlex
Anastasiia Gainullina, Denis A. Mogilenko, Li‐Hao Huang, Helena Todorov et autres
The diversity of mononuclear phagocyte (MNP) subpopulations across tissues is one of the key physiological characteristics of the immune system. Here, we focus on understanding the metabolic variability of MNPs through metabolic network analysis applied to three large-scale transcriptional datasets: we introduce …
ru, us, be, sg, fr, de, tw
(code pays fourni par la source)
Accès ouvert
2022
preprint
OpenAlex
Helena Todorov, Tania Miguel Trabajo, Jan Roelof van der Meer
Abstract Bacterial growth can be studied at the single cell-level through time-lapse microscopy imaging. Technical advances in microscopy lead to increasing image quality, which in turn allows to visualize larger areas of growth, containing more and more cells. In this context, the …
ch
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Helena Todorov, Margaux Prieux, Daphné Laubreton, Matteo Bouvier et autres
In this work, we studied the generation of memory precursor cells following an acute infection by analyzing single-cell RNA-seq data that contained CD8 T cells collected during the postinfection expansion phase. We used different tools to reconstruct the developmental trajectory that CD8 …
be, fr
(code pays fourni par la source)
Accès ouvert
2022
article
OpenAlex
Laetitia Dubouchet, Helena Todorov, Ruth Seurinck, Nicolas Vallet et autres
The mechanisms underlying operational tolerance after hematopoietic stem cell transplantation in humans are poorly understood. We studied two independent cohorts of patients who underwent allogeneic hematopoietic stem cell transplantation from human leukocyte antigen–identical siblings. Primary tolerance was associated with long-lasting reshaping of …
fr, be, us
(code pays fourni par la source)
Accès ouvert
2022
preprint
OpenAlex
Helena Todorov, Margaux Prieux, Daphné Laubreton, Matteo Bouvier et autres
Abstract In this work, we studied the generation of memory precursor cells following an acute infection by analyzing single-cell RNA-seq data that contained CD8 T cells collected during the postinfection expansion phase. We used different tools to reconstruct the developmental trajectory that …
fr, be
(code pays fourni par la source)