Yuki Akieda

Endogenous cell competition kills pathogenic de novo mutant cells but declines under pH stress

Yuki Akieda, Hikari Ukawa, Natsuki Watanabe, Tohru Ishitani

Abstract Although endogenous cell competition safeguards embryonic development by eliminating unfit cells, the intrinsic origins of unfitness remain unclear. Here, zebrafish whole-genome sequencing and imaging reveal that endogenous cell competition removes cells with spontaneous de novo mutations in key developmental signalling pathways …

jp (code pays fourni par la source)

Recovery of infectious recombinant human norovirus using zebrafish embryos

Tomohiro Kotaki, Yuki Akieda, Zelin Chen, M. Onishi et autres

Human norovirus (HuNoV) is the leading cause of gastroenteritis. However, the lack of a reverse genetics system for infectious HuNoV has hindered the development of antivirals and vaccines. Herein, we established a reverse genetics system for infectious HuNoV using a robust HuNoV …

jp (code pays fourni par la source)

Foxo3-mediated physiological cell competition ensures robust tissue patterning throughout vertebrate development

Kanako Matsumoto, Yuki Akieda, Yukinari Haraoka, Naoki Hirono et autres

Unfit cells with defective signalling or gene expression are eliminated through competition with neighbouring cells. However, physiological roles and mechanisms of cell competition in vertebrates remain unclear. In addition, universal mechanisms regulating diverse cell competition are unknown. Using zebrafish imaging, we reveal …

jp (code pays fourni par la source)

Mechano-gradients drive morphogen-noise correction to ensure robust patterning

Kana Aoki, Yuki Akieda, Yasuyuki Ohkawa, Tohru Ishitani

Morphogen gradients instruct cells to pattern tissues. Although the mechanisms by which morphogens transduce chemical signals have been extensively studied, the roles and regulation of the physical communication between morphogen-receiver cells remain unclear. Here, we show that the Wnt/β-catenin-morphogen gradient, which patterns …

jp (code pays fourni par la source)

Calcium sparks enhance the tissue fluidity within epithelial layers and promote apical extrusion of transformed cells

Keisuke Kuromiya, Kana Aoki, Kojiro Ishibashi, Moe Yotabun et autres

In vertebrates, newly emerging transformed cells are often apically extruded from epithelial layers through cell competition with surrounding normal epithelial cells. However, the underlying molecular mechanism remains elusive. Here, using phospho-SILAC screening, we show that phosphorylation of AHNAK2 is elevated in normal …

jp (code pays fourni par la source)

Zebrafish imaging reveals TP53 mutation switching oncogene-induced senescence from suppressor to driver in primary tumorigenesis

Yukinari Haraoka, Yuki Akieda, Yuri Nagai, Chihiro Mogi et autres

Abstract Most tumours are thought to arise through oncogenic cell generation followed by additional mutations. How a new oncogenic cell primes tumorigenesis by acquiring additional mutations remains unclear. We show that an additional TP53 mutation stimulates primary tumorigenesis by switching oncogene-induced senescence …

jp (code pays fourni par la source)

A novel role for PRL in regulating epithelial cell density by inducing apoptosis at confluence

Sweksha Lohani, Yosuke Funato, Yuki Akieda, Kiyohito Mizutani et autres

Maintaining proper epithelial cell density is essential for the survival of multicellular organisms. Although regulation of cell density through apoptosis is well known, its mechanistic details remain elusive. Here, we report the involvement of membrane-anchored phosphatase of regenerating liver (PRL), originally known …

jp (code pays fourni par la source)

Calcium Wave Promotes Cell Extrusion

Yasuto Takeuchi, Rika Narumi, Ryutaro Akiyama, Elisa Vitiello et autres

jp, gb, sg (code pays fourni par la source)

Zebrafish imaging analysis revealed a novel function and control of cell competition

Yuki Akieda, Yukinari Haraoka, Shohei Ogamino, Tohru Ishitani

gb (code pays fourni par la source)

Defective cell sensing and elimination system utilizing Wnt morphogen-gradient

Yuki Akieda, Shohei Ogamino, Tohru Ishitani

gb (code pays fourni par la source)

Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo

Yuki Akieda, Shohei Ogamino, Hironobu Furuie, Shizuka Ishitani et autres

Morphogen signalling forms an activity gradient and instructs cell identities in a signalling strength-dependent manner to pattern developing tissues. However, developing tissues also undergo dynamic morphogenesis, which may produce cells with unfit morphogen signalling and consequent noisy morphogen gradients. Here we show …

jp (code pays fourni par la source)

Live-imaging Analyses Using Small Fish Models Reveal New Mechanisms That Regulate Primary Tumorigenesis

Yukinari Haraoka, Yuki Akieda, Tohru Ishitani

Since the 1980s, zebrafish (Danio rerio) have been used as a valuable model system to investigate developmental processes because they: 1) grow outside their mothers; 2) are transparent during the embryonic stage; and 3) have organs similar to those in humans. Recently, …

jp (code pays fourni par la source)