James B. Kaper

Flagellar regulation in enterotoxigenic E. coli modulates innate immune activation

Pauline T. Ikpa, Amanda Nengel, James B. Kaper, Mark Donowitz et autres

ABSTRACT Enterotoxigenic Escherichia coli (ETEC) remains a leading cause of diarrheal disease in low- and middle-income countries, yet vaccine efforts have struggled to achieve broad and durable protection. To better understand early steps in host-pathogen interactions, we utilized human stem cell-derived jejunal …

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A history of EcoSal Plus

James B. Kaper

ABSTRACT EcoSal Plus (ESP) is the authoritative online review journal that publishes an ever-growing body of expert reviews covering virtually all aspects of Escherichia coli , Salmonella , and other members of the order Enterobacterales and their use as model microbes for …

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Mo1874 HOST EPITHELIAL ADHERENCE AND CXCL5/CXCR2 MEDIATE INTESTINAL MACROPHAGE RECOGNITION OF ENTEROTOXIGENIC E. COLI

Pauline T. Ikpa, Eileen M. Barry, James B. Kaper, Mark Donowitz et autres

Mucosal Immune Profiles Associated with Diarrheal Disease Severity inShigella- and Enteropathogenic Escherichia coli-Infected Children Enrolled in the Global Enteric Multicenter Study

Amanda D. Buskirk, Esther Ndungo, Avital A. Shimanovich, Diana Lam et autres

with and without dysentery, and controls in well characterized clinical samples obtained during the Global Enteric Multicenter Study. For the first time, we report pathogen-specific mucosal immune profiles associated with severity or absence of disease in children <5 years of age that …

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Cross-Talk between Probiotic Nissle 1917 and Human Colonic Epithelium Affects the Metabolite Composition and Demonstrates Host Antibacterial Effect

Karol Dokładny, John K. Crane, Alex J. Kassicieh, James B. Kaper et autres

Colonic epithelium–commensal interactions play a very important role in human health and disease development. Colonic mucus serves as an ecologic niche for a myriad of commensals and provides a physical barrier between the epithelium and luminal content, suggesting that communication between the …

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Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing Escherichia coli–Host Interactions

Karol Dokładny, Julie In, James B. Kaper, Olga Kovbasnjuk

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Coronin-1 is necessary for enteric pathogen-induced transcytosis across human ileal enteroid monolayers expressing M cells

Janet F. Staab, Michèle Doucet, Rachel Latanich, Sun Lee et autres

ABSTRACT In the intestine, luminal sampling by microfold (M) cells is crucial for inducing protective mucosal immune responses but can also serve as an entry pathway for pathogens, including bacteria and viruses. Enteric pathogens can influence intestinal M cell function; however, the …

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Phosphodiesterase 5 (PDE5) restricts intracellular cGMP accumulation during enterotoxigenic Escherichia coli infection

Jennifer Foulke‐Abel, Huimin Yu, Laxmi Sunuwar, Ruxian Lin et autres

(ETEC) has a continuing impact on residents and travelers in underdeveloped countries. Both heat-labile (LT) and heat-stable (ST) enterotoxins contribute to pathophysiology via induction of cyclic nucleotide synthesis, and previous investigations focused on intracellular signal transduction rather than possible intercellular second messenger …

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Mechanical Stimuli Affect Escherichia coli Heat-Stable Enterotoxin-Cyclic GMP Signaling in a Human Enteroid Intestine-Chip Model

Laxmi Sunuwar, Jianyi Yin, Magdalena Kasendra, Katia Karalis et autres

heat-stable enterotoxin A (ST) and evaluated under conditions of static fluid, apical and basolateral flow, and flow plus repetitive stretch. Application of flow increased epithelial cell height and apical and basolateral secretion of cyclic GMP (cGMP) under baseline, unstimulated conditions. Addition of …

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Su1943 – Long-Term Ehec Infection Stimulates Mucus Production in Human Stem Cell-Derived Colonoids Possibly Via Atoh1 Activation

Karol Dokładny, Anne‐Marie Hansen, James B. Kaper, Olga Kovbasnjuk

Su1012 – Mechanical Stimuli Affect Enterotoxigenic E. Coli (ETEC) Heat Stable Enterotoxin (ST)-Cyclic Nucleotide Signaling by Altering Mrp4 Expression in a Human Enteroid Monolayer Model of Etec Diarrhea

Laxmi Sunuwar, Jianyi Yin, Magdalena Kasendra, Katia Karalis et autres

Enterohemorrhagic E. coli (EHEC)—Secreted Serine Protease EspP Stimulates Electrogenic Ion Transport in Human Colonoid Monolayers

Chung‐Ming Tse, Julie In, Jianyi Yin, Mark Donowitz et autres

One of the characteristic manifestations of Shiga-toxin-producing Escherichia coli (E. coli) infection in humans, including EHEC and Enteroaggregative E. coli O104:H4, is watery diarrhea. However, neither Shiga toxin nor numerous components of the type-3 secretion system have been found to independently elicit …

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