Monique van Straaten

Affinity-independent memory B cell origin of the early antibody-secreting cell response in naive individuals upon SARS-CoV-2 vaccination

Zhe Li, Anna Obraztsova, Fuwei Shang, Opeyemi Ernest Oludada et autres

Memory B cells (MBCs) formed over the individual's lifetime constitute nearly half of the circulating B cell repertoire in humans. These pre-existing MBCs dominate recall responses to their cognate antigens, but how they respond to recognition of novel antigens is not well …

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Memory B cells dominate the early antibody-secreting cell response to SARS-CoV-2 mRNA vaccination in naïve individuals independently of their antibody affinity

Zhe Li, Anna Obraztsova, Fuwei Shang, Opeyemi Ernest Oludada et autres

Abstract Memory B cells (MBCs) formed over the individual’s lifetime constitute nearly half of the adult peripheral blood B cell repertoire in humans. To assess their response to novel antigens, we tracked the origin and followed the differentiation paths of MBCs in …

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A structural classification of the variant surface glycoproteins of the African trypanosome

Sara Đaković, Johan Zeelen, Anastasia Gkeka, Monica Chandra et autres

Long-term immune evasion by the African trypanosome is achieved through repetitive cycles of surface protein replacement with antigenically distinct versions of the dense Variant Surface Glycoprotein (VSG) coat. Thousands of VSG genes and pseudo-genes exist in the parasite genome that, together with …

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Immunodominant surface epitopes power immune evasion in the African trypanosome

Anastasia Gkeka, Francisco Aresta‐Branco, Gianna Triller, Evi P. Vlachou et autres

The African trypanosome survives the immune response of its mammalian host by antigenic variation of its major surface antigen (the variant surface glycoprotein or VSG). Here we describe the antibody repertoires elicited by different VSGs. We show that the repertoires are highly …

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Structural similarities between the metacyclic and bloodstream form variant surface glycoproteins of the African trypanosome

Monica Chandra, Sara Đaković, Konstantina Foti, Johan Zeelen et autres

During infection of mammalian hosts, African trypanosomes thwart immunity using antigenic variation of the dense Variant Surface Glycoprotein (VSG) coat, accessing a large repertoire of several thousand genes and pseudogenes, and switching to antigenically distinct copies. The parasite is transferred to mammalian …

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A Structural Classification of the Variant Surface Glycoproteins of the African Trypanosome

Sara Đaković, Johan Zeelen, Anastasia Gkeka, Monica Chandra et autres

ABSTRACT Long-term immune evasion by the African trypanosome is achieved through repetitive cycles of surface protein replacement with antigenically distinct versions of the dense Variant Surface Glycoprotein (VSG) coat. Thousands of VSG genes and pseudo-genes exist in the parasite genome that, together …

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A trypanosome-derived immunotherapeutics platform elicits potent high-affinity antibodies, negating the effects of the synthetic opioid fentanyl

Gianna Triller, Evi P. Vlachou, Hamidreza Hashemi, Monique van Straaten et autres

Poorly immunogenic small molecules pose challenges for the production of clinically efficacious vaccines and antibodies. To address this, we generate an immunization platform derived from the immunogenic surface coat of the African trypanosome. Through sortase-based conjugation of the target molecules to the …

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Structural and Immunological Similarities Between the Metacyclic and Bloodstream Form Variant Surface Glycoproteins of the African Trypanosome

Monica Chandra, Sara Đaković, Konstantina Foti, Johan Zeelen et autres

Abstract During infection of mammalian hosts, African trypanosomes thwart immunity using antigenic variation of the dense Variant Surface Glycoprotein (VSG) coat, accessing a large repertoire of thousands of genes and pseudogenes and switching to antigenically distinct copies. The parasite is transferred to …

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An Epitope-Focused Trypanosome-Derived Vaccine Platform Elicits High-Affinity Antibodies and Immunity Against Fentanyl Effects

Gianna Triller, Evi P. Vlachou, Hamidreza Hashemi, Monique van Straaten et autres

Summary Poorly antigenic small molecules pose challenges for the production of clinically efficacious antibodies. To address this, we have developed an immunization platform derived from the antigenic surface coat of the African trypanosome. Through sortase-based conjugation of antigens to the trypanosome surface …

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Translational control of E2f1 regulates the Drosophila cell cycle

Jan Inge Øvrebø, Mary-Rose Bradley-Gill, Norman Zielke, Minhee Kim et autres

Significance Here, we explore a mechanism wherein the growth signaling-dependent translation of mRNA encoding a cell cycle regulatory factor limits rates of cell proliferation. An essential cell cycle transcription factor, E2F1, was found to be post-transcriptionally regulated by TOR and EGFR signaling, …

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An Epitope-Focused Trypanosome-Derived Vaccine Platform Elicits High-Affinity Antibodies and Immunity Against Fentanyl Effects

Gianna Triller, Evi P. Vlachou, Hamidreza Hashemi, Monique van Straaten et autres

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Nanobody-mediated macromolecular crowding induces membrane fission and remodeling in the African trypanosome

A. Hempelmann, Laura Hartleb, Monique van Straaten, Hamidreza Hashemi et autres

The dense variant surface glycoprotein (VSG) coat of African trypanosomes represents the primary host-pathogen interface. Antigenic variation prevents clearing of the pathogen by employing a large repertoire of antigenically distinct VSG genes, thus neutralizing the host's antibody response. To explore the epitope …

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