Juliette Van Steenwinckel

Microglial maturation across human and mouse as a reference for interpreting large-animal models of perinatal brain injury

Isabelle K. Shearer, Adrienne M. Antonson, Juliette Van Steenwinckel, Pierre Gressens et autres

Microglia play dynamic roles in the developing brain and are central mediators of injury responses in perinatal brain injury. In mice, microglial gene regulatory and transcriptional programes reveal progression through early, pre-mature and mature stages. In contrast, microglial maturation has not been …

au, us, fr (code pays fourni par la source)

Spatiotemporal transcriptomic mapping of epileptogenesis

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

International audience

Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Temporal lobe epilepsy (TLE) is a prevalent neurological disorder often preceded by an initial precipitating event, followed by a latent phase, and culminating in chronic epilepsy with recurrent seizures. The molecular and cellular mechanisms driving this transformation remain incompletely understood. Here, we …

fr (code pays fourni par la source)

Additional file 13 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Supplementary Material 13: Supplementary Table 4. Differentially expressed genes between SE and control samples. Cortex and hippocampus sub-clusters and clusters 1, 2 and 4 were considered for treated vs control samples comparisons. Differentially expressed genes were identified for all the clusters and …

fr (code pays fourni par la source)

Additional file 3 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Supplementary Material 3: Supplementary Fig. 3. Sub-clustering of the cortex. A Four panels of UMAP representation of cortical cluster spots colored according to subcluster, condition (treated or control), time points or sample of origin (from top to bottom and from left to …

fr (code pays fourni par la source)

Additional file 5 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Supplementary Material 5: Supplementary Fig. 5. Visualisation of conditions and samples repartition by clusters. A Conditions repartition by clusters. On the left, the repartition of the different clusters for each condition and time, with the number of spots for each condition on …

fr (code pays fourni par la source)

Additional file 9 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Supplementary Material 9: Supplementary Fig. 9. Cell-type composition by section. Barplots representing the cumulative estimated proportions of cell-types for all spots of each section.

fr (code pays fourni par la source)

Additional file 9 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Supplementary Material 9: Supplementary Fig. 9. Cell-type composition by section. Barplots representing the cumulative estimated proportions of cell-types for all spots of each section.

fr (code pays fourni par la source)

Additional file 11 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Supplementary Material 11: Supplementary Table 2. Marker genes of clusters and subclusters. One cluster/subcluster vs all other clusters/subclusters comparisons were made to identify marker genes.

fr (code pays fourni par la source)

Additional file 2 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Supplementary Material 2: Supplementary Fig. 2. Whole clustering. A Four panels of UMAP representation of all spots passing quality control colored according to clusters, condition (treated or control), time points or sample of origin (from top to bottom and from left to …

fr (code pays fourni par la source)

Additional file 1 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Supplementary Material 1: Supplementary Fig. 1. Histological and quality control visualisation. Hematoxylin-and-eosin-stained tissue sections and corresponding spatial distribution of UMI counts per spot for each sample.

fr (code pays fourni par la source)

Additional file 5 of Spatiotemporal transcriptomic mapping reveals region-specific glial activation and astrocyte shifts in epileptogenesis beyond the hippocampus

Adrien Dufour, Christophe Le Priol, Baptiste Porte, Ronan Jouanard et autres

Supplementary Material 5: Supplementary Fig. 5. Visualisation of conditions and samples repartition by clusters. A Conditions repartition by clusters. On the left, the repartition of the different clusters for each condition and time, with the number of spots for each condition on …

fr (code pays fourni par la source)